FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004717676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1302295
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT75700.5812815068782419No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT62740.48176488430040815No Hit
GATCTATGCTGCAACCAATTTGCAAGGTGGGGTCCCACCTAGGTTCAGTG52520.40328804149597447No Hit
GTACTATTGTCAACAGTCTAACGGATATCCTCGGACGTTCGGCCCAGGGA47600.36550858292475974No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCC47150.3620531446408072No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC46520.3572155310432736No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA44250.33978476458866846No Hit
ATTAATACCTGGTTAGCCTGGTATCAGCACAAACCTGGGAAAGCCCCTAG42730.32811306194065093No Hit
CCGTTAGACTGTTGACAATAGTACGTTGCAAAATCCTCAGGCTGGAGGTTGCTGATTGTGAGACTGAAATCTGTT41280.3169788719145816No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT40650.312141258317048No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG39350.3021588810522961No Hit
GTCTAACGGATATCCTCGGACGTTCGGCCCAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT38400.29486406689728517No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT37690.28941215316038227No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37510.28802997784680123No Hit
GTTGACAATAGTACGTTGCAAAATCCTCAGGCTGGAGGTTGCTGATTGTGAGACTGAAATCTGTTCCAGAGCC34550.26530087269013547No Hit
ATTGTCAACAGTCTAACGGATATCCTCGGACGTTCGGCCCAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTG34410.26422584744623917No Hit
GTCTCCATCCTCTGTGTCTGCGTCTGTAGGAGACACAGTCACCATCACTTGTCGGGCGAGTCAGTCTATTAATAC33550.2576221209480187No Hit
CAATAGTACGTTGCAAAATCCTCAGGCTGGAGGTTGCTGATTGTGAGACTGAAATCTGTTCCAGAGCCACTGCC33340.2560095830821742No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC32570.25009694424074425No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA32530.24978979417105956No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG31480.2417271048418369No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTGGGCCGAACGTCCGAGGATATCCGTTAGACTGTTGAC30590.23489301579135294No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG29470.22629281384018213No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC29450.22613923880533982No Hit
GTCTATTAATACCTGGTTAGCCTGGTATCAGCACAAACCTGGGAAAGCCCCTAGGCCCCTGATCTATGCTGCAAC28650.21999623741164637No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28500.21884442465032886No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28450.21846048706322302No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC28450.21846048706322302No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28020.2151586238141128No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT27930.21446753615732228No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC26210.20126008316088137No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT25590.19649925708076893No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC25470.19557780687171492No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA24890.19112413086128718No Hit
GCATAGATCAGGGGCCTAGGGGCTTTCCCAGGTTTGTGCTGATACCAGGC24820.190586618239339No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24620.18905086789091566No Hit
GGCTGGAGGTTGCTGATTGTGAGACTGAAATCTGTTCCAGAGCCACTGCC24460.18782226761217696No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23890.1834453791191704No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC23880.1833685916017492No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT23790.1826775039449587No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT23400.17968279076553315No Hit
ACCTAGGTGGGACCCCACCTTGCAAATTGGTTGCAGCATAGATCAGGGGCCTAGGGGCTTTCCCAGGTTTGTG23170.1779166778648463No Hit
CCTCCAGCCTGAGGATTTTGCAACGTACTATTGTCAACAGTCTAACGGATATCCTCGGACGTTCGGCCCAGGGAC22380.171850463988574No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG22210.17054507619241416No Hit
CCCCTGATCTATGCTGCAACCAATTTGCAAGGTGGGGTCCCACCTAGGTT22100.1697004135007813No Hit
GCCCAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGC21250.16317347451998204No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCC21200.16278953693287618No Hit
CACCTAGGTTCAGTGGCAGTGGCTCTGGAACAGATTTCAGTCTCACAATCAGCAACCTCCAGCCTGAGGATTTTG21080.16186808672382216No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC20440.1569536856088674No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC20400.15664653553918276No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT20340.15618581043465574No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20030.15380539739459953No Hit
GGTATTAATAGACTGACTCGCCCGACAAGTGATGGTGACTGTGTCTCCTACAGACGCAGACACAGAGGATGG19730.1515017718719645No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC18850.14474447033890173No Hit
GATCAGGGGCCTAGGGGCTTTCCCAGGTTTGTGCTGATACCAGGCTAACCAGGTATTAATAGACTGACTCGCCCG18810.14443732026921705No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC18620.14297835743821485No Hit
GCACAAACCTGGGAAAGCCCCTAGGCCCCTGATCTATGCTGCAACCAATTTGCAAGGTGGGGTCCCACCTAGGTT18440.14159618212463382No Hit
GTGGGGTCCCACCTAGGTTCAGTGGCAGTGGCTCTGGAACAGATTTCAGTCTCACAATCAGCAACCTCCAGCCTG18410.14136581957237032No Hit
GGTTTGTGCTGATACCAGGCTAACCAGGTATTAATAGACTGACTCGCCCGACAAGTGATGGTGACTGTGTCTCCT18290.1404443693633163No Hit
GTAGGAGACACAGTCACCATCACTTGTCGGGCGAGTCAGTCTATTAATACCTGGTTAGCCTGGTATCAGCACAA18170.1395229191542623No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC17840.13698893107936375No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17720.13606748087030973No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17610.13522281817867687No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17480.13422458045220167No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT17430.13384064286509587No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG17200.132074529964409No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17110.13138344230761848No Hit
GTGTCTGCGTCTGTAGGAGACACAGTCACCATCACTTGTCGGGCGAGTCA17050.13092271720309148No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC16340.12547080346618852No Hit
AACCTGGGAAAGCCCCTAGGCCCCTGATCTATGCTGCAACCAATTTGCAA16160.12408862815260752No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16150.12401184063518635No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCCAGATG15940.12239930276934181No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15940.12239930276934181No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15870.12186179014739365No Hit
GTTCCAGAGCCACTGCCACTGAACCTAGGTGGGACCCCACCTTGCAAATTGGTTGCAGCATAGATCAGGGGCCT15710.12063318986865496No Hit
CTATTAATACCTGGTTAGCCTGGTATCAGCACAAACCTGGGAAAGCCCCTAGGCCCCTGATCTATGCTGCAACCA15640.12009567724670678No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCC15600.1197885271770221No Hit
CCTAGGTTCAGTGGCAGTGGCTCTGGAACAGATTTCAGTCTCACAATCAGCAACCTCCAGCCTGAGGATTTTGCA15600.1197885271770221No Hit
GTCCCTGGGCCGAACGTCCGAGGATATCCGTTAGACTGTTGACAATAGTA15530.11925101455507393No Hit
ACCCAGTCTCCATCCTCTGTGTCTGCGTCTGTAGGAGACACAGTCACCATCACTTGTCGGGCGAGTCAGTCTATT15460.11871350193312574No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15420.11840635186344108No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15320.1176384766892294No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA15250.11710096406728124No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC15200.11671702648017537No Hit
TTAATAGACTGACTCGCCCGACAAGTGATGGTGACTGTGTCTCCTACAGA15040.11548842620143669No Hit
AGGTGGGACCCCACCTTGCAAATTGGTTGCAGCATAGATCAGGGGCCTAGGGGCTTTCCCAGGTTTGTGCTGAT14860.11410625088785567No Hit
GTATTAATAGACTGACTCGCCCGACAAGTGATGGTGACTGTGTCTCCTACAGACGCAGACACAGAGGATGG14460.11103475019100895No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCC14390.11049723756906078No Hit
CTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCCAGATGACCCAGTCTCCATCCTCTGTGTCTGCGTCTGT14390.11049723756906078No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC14140.10857754963353157No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14100.1082703995638469No Hit
CCTTGGTCCCTGGGCCGAACGTCCGAGGATATCCGTTAGACTGTTGACAATAGTACGTTGCAAAATCCTCAGG13960.10719537431995055No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT13940.10704179928510821No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13910.10681143673284471No Hit
GTCCCACCTAGGTTCAGTGGCAGTGGCTCTGGAACAGATTTCAGTCTCAC13840.10627392411089653No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13680.10504532383215784No Hit
CTCTGGAACAGATTTCAGTCTCACAATCAGCAACCTCCAGCCTGAGGATTTTGCAACGTACTATTGTCAACAGT13490.10358636100115566No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13360.10258812327468046No Hit
GCCTGGTATCAGCACAAACCTGGGAAAGCCCCTAGGCCCCTGATCTATGCTGCAACCAATTTGCAAGGTGGGGTC13350.1025113357572593No Hit
GTTCAGTGGCAGTGGCTCTGGAACAGATTTCAGTCTCACAATCAGCAACCTCCAGCCTGAGGATTTTGCAACGT13240.10166667306562645No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT13160.1010523729262571No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13160.1010523729262571No Hit
CTCTGTGTCTGCGTCTGTAGGAGACACAGTCACCATCACTTGTCGGGCGAGTCAGTCTATTAATACCTGGTTAGC13150.10097558540883594No Hit
GAACCTAGGTGGGACCCCACCTTGCAAATTGGTTGCAGCATAGATCAGGGGCCTAGGGGCTTTCCCAGGTTTGTG13080.10043807278688777No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)