FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1114439
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63090.5661144306687041No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT61220.5493346876769387No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCATCATAATGGATACAACTATTTGG44910.4029830255402046No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41000.36789810837560427No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG40310.36170665240538064No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG37400.3355948598353073No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36500.32751904770023305No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34960.31370043582466156No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA34790.312175004643592No Hit
CTACAAAGCCCATTCACCTTCGGCCCTGGGACCAAAGTGGAAATCATACGAACTGTGGCTGCACCATCTGTCTTC33550.30104833014637855No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT33060.2966514990950604No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT31540.28301234971137945No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAAATTTTTCACTGAA31130.2793333686276234No Hit
GGTCTAGTCAGAGCCTCCTGCATCATAATGGATACAACTATTTGGATTGGTACTTACAGAAGCCAGGGCAGTCTC30940.2776284749546633No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA30330.27215486895200186No Hit
GTGCAGCCACAGTTCGTATGATTTCCACTTTGGTCCCAGGGCCGAAGGTGAATGGGCTTTGTAGAGCTTGCATGC30160.2706294377709323No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA28850.258874644552102No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC28660.25716975087914185No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG28570.25636216966563447No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGG27210.24415872021707782No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATACATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGTAAGT26620.23886457670630692No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25390.22782763345503884No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT24800.22253348994426794No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTATGATTTCCACTTTGGTCC24470.21957235882807402No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC24260.21768800266322336No Hit
GTTCAGTGGCAGTGGATCAGGCACAAATTTTTCACTGAAAATCAGCAGAC23940.21481660279297474No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23890.21436794656324842No Hit
GTAATAAATACCAACATCCTCAGCCTCCAGTCTGCTGATTTTCAGTGAAAAATTTGTGCCTGATCCACTGCC22910.205574284460612No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22800.20458724075521406No Hit
TTGTAGAGCTTGCATGCAGTAATAAATACCAACATCCTCAGCCTCCAGTC21990.19731900983364722No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATGTATTTGGGTTCTAATCGGGCC20890.18744857277966762No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20730.1860128728445433No Hit
CTCCACAACTCCTGATGTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCT20520.18412851667969266No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATGC20510.18403878543374738No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC20460.18359012920402104No Hit
GCTAATGCTCTGGGTCTCTGGATGCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC20380.18287227923645888No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20360.18269281674456833No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCATCATAATGG20190.18116738556349876No Hit
CCTTCGGCCCTGGGACCAAAGTGGAAATCATACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG19930.17883437316892176No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT19910.17865491067703124No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC19660.1764116295283995No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATGCAGTGGGGATATTGTGATG19550.17542458582300152No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19130.17165587349330022No Hit
TTATTACTGCATGCAAGCTCTACAAAGCCCATTCACCTTCGGCCCTGGGA19000.17048936729601172No Hit
CAGTAATAAATACCAACATCCTCAGCCTCCAGTCTGCTGATTTTCAGTGAAAAATTTGTGCCTGATCCACTGCC18610.1669898487041462No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT18270.16393898634200704No Hit
GTAGTAGACGGCCGTGTCCGCAGGGGTCACAGAGGTCAGCTTCAGGGAGAACTGGTTCCTGGACATTTCCACTG18100.16241355516093747No Hit
GGATACAACTATTTGGATTGGTACTTACAGAAGCCAGGGCAGTCTCCACAACTCCTGATGTATTTGGGTTCTAAT17710.15891403656907196No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATGCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC17610.15801672410961928No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT17530.15729887414205712No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC17490.15693994915827605No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17370.1558631742069328No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17300.15523505548531594No Hit
GTACTTACAGAAGCCAGGGCAGTCTCCACAACTCCTGATGTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTG17220.15451720551775378No Hit
GGCTTTGTAGAGCTTGCATGCAGTAATAAATACCAACATCCTCAGCCTCC17220.15451720551775378No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16910.15173553689345043No Hit
GTTGTGGAGACTGCCCTGGCTTCTGTAAGTACCAATCCAAATAGTTGTATCCATTATGATGCAGGAGGCTCTG16850.15119714941777881No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT16730.15012037446643559No Hit
ATTTATTACTGCATGCAAGCTCTACAAAGCCCATTCACCTTCGGCCCTGGGACCAAAGTGGAAATCATACGA16670.14958198699076397No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC16550.14850521203942074No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16500.14805655580969437No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA16340.14662085587457008No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC16250.14581327466106264No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATGCAGTGGGGATATTGTGATGACTCAGTCTC15810.14186509983947082No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15680.1406985936421823No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATGCAGTGGGGATATTGTGATGACTCAGTCTCCACT15670.14060886239623702No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT15590.13989101242867488No Hit
GCTCTACAAAGCCCATTCACCTTCGGCCCTGGGACCAAAGTGGAAATCATACGAACTGTGGCTGCACCAT15380.13800665626382422No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15230.13666068757464517No Hit
GCCCTGGCTTCTGTAAGTACCAATCCAAATAGTTGTATCCATTATGATGCAGGAGGCTCTGACTAGACCTGCAGG15050.13504552514763032No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG15040.13495579390168505No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14770.1325330502611628No Hit
AGTCAGAGCCTCCTGCATCATAATGGATACAACTATTTGGATTGGTACTTACAGAAGCCAGGGCAGTCTCCACA14590.13091788783414796No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14390.12912326291524254No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC14350.1287643379314615No Hit
GCCACAGTTCGTATGATTTCCACTTTGGTCCCAGGGCCGAAGGTGAATGG14290.12822595045578988No Hit
GTGAATGGGCTTTGTAGAGCTTGCATGCAGTAATAAATACCAACATCCTC14200.12741836924228245No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14190.12732863799633717No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG14060.12616213179904867No Hit
GCCCATTCACCTTCGGCCCTGGGACCAAAGTGGAAATCATACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC13920.12490589435581491No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA13780.12364965691258113No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13640.12239341946934737No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13630.1223036882234021No Hit
GGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGG13600.12203449448556629No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13480.12095771953422306No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTC13460.1207782570423325No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT13420.12041933205855145No Hit
CTGTAAGTACCAATCCAAATAGTTGTATCCATTATGATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGC13230.11871443838559131No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13190.11835551340181023No Hit
CTCCTGCATCATAATGGATACAACTATTTGGATTGGTACTTACAGAAGCC13160.11808631966397443No Hit
CCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCATCAT13100.11754793218830281No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13090.11745820094235754No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC13020.11683008222074066No Hit
GTCTCCACAACTCCTGATGTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGATC12980.11647115723695958No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12930.11602250100723323No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC12860.11539438228561635No Hit
CTCCTGATGTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTT12810.11494572605589No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12400.11126674497213397No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT12330.11063862625051708No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT12300.11036943251268128No Hit
GGCCGAAGGTGAATGGGCTTTGTAGAGCTTGCATGCAGTAATAAATACCA12280.11018997002079073No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTC12080.10839534510188535No Hit
GGGTCTCTGGATGCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGG12030.10794668887215898No Hit
CATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGTAAGTACCAATCCAAAT11890.10669045142892522No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT11730.10525475149380091No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT11730.10525475149380091No Hit
ACAGAAGCCAGGGCAGTCTCCACAACTCCTGATGTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTT11690.10489582651001983No Hit
GGTACTTACAGAAGCCAGGGCAGTCTCCACAACTCCTGATGTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTG11660.10462663277218402No Hit
CAACTATTTGGATTGGTACTTACAGAAGCCAGGGCAGTCTCCACAACTCCTGATGTATTTGGGTTCTAATCGGGC11620.10426770778840297No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11620.10426770778840297No Hit
GTTCGTATGATTTCCACTTTGGTCCCAGGGCCGAAGGTGAATGGGCTTTGTAGAGCTTGCATGCAGTAATAAAT11550.10363958906678607No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCC11460.10283200785327865No Hit
GTCTACTACTGTGCGAGAGGACAATTTTTGGAGTGGTTACCTATCTTACCGGCGTGGAGACTTGACTCCTGGGGC11390.10220388913166176No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG11310.10148603916409961No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG11270.10112711418031853No Hit
GCAGTGGATCAGGCACAAATTTTTCACTGAAAATCAGCAGACTGGAGGCT11150.1000503392289753No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)