FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1114439
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60870.5461940940688543No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT52400.47019172875321125No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCATCATAATGGATACAACTATTTGG40930.3672699896539873No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA38900.3490545467270977No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38590.34627287810279433No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33920.30436838624635354No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG33320.2989845114896374No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32990.2960233803734435No Hit
CTACAAAGCCCATTCACCTTCGGCCCTGGGACCAAAGTGGAAATCATACGAACTGTGGCTGCACCATCTGTCTTC32320.2900113868951104No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT31420.2819355747600362No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT29200.26201523816018646No Hit
GGTCTAGTCAGAGCCTCCTGCATCATAATGGATACAACTATTTGGATTGGTACTTACAGAAGCCAGGGCAGTCTC28820.2586054508142662No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC28260.2535805010413311No Hit
GTGCAGCCACAGTTCGTATGATTTCCACTTTGGTCCCAGGGCCGAAGGTGAATGGGCTTTGTAGAGCTTGCATGC27770.2491836699900129No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27380.24568415139814742No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA27220.24424845146302313No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAAATTTTTCACTGAA27200.24406898897113258No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGG26460.23742887677118263No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG26190.23500613313066038No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG25920.23258338949013807No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24850.22298214617399426No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC24480.21966209007401932No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24210.21723934643349704No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATACATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGTAAGT24030.21562418400648217No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTATGATTTCCACTTTGGTCC23140.2076381031173532No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23090.2071894468876269No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC22950.2059332094443931No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT22930.20575374695250256No Hit
GTTCAGTGGCAGTGGATCAGGCACAAATTTTTCACTGAAAATCAGCAGAC22890.2053948219687215No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCATCATAATGG22360.2006390659336222No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA21760.19525519117690604No Hit
CTCCACAACTCCTGATGTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCT21330.19139674760125946No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21120.18951239143640883No Hit
GTAATAAATACCAACATCCTCAGCCTCCAGTCTGCTGATTTTCAGTGAAAAATTTGTGCCTGATCCACTGCC21100.18933292894451828No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC21070.18906373520668246No Hit
TTGTAGAGCTTGCATGCAGTAATAAATACCAACATCCTCAGCCTCCAGTC20930.1878074977634487No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATGC20370.1827825479905136No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT20200.18125711680944404No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATGTATTTGGGTTCTAATCGGGCC19970.17919329815270285No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19550.17542458582300152No Hit
GGCTTTGTAGAGCTTGCATGCAGTAATAAATACCAACATCCTCAGCCTCC19050.17093802352573806No Hit
GCTAATGCTCTGGGTCTCTGGATGCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC18740.1681563549014347No Hit
TTATTACTGCATGCAAGCTCTACAAAGCCCATTCACCTTCGGCCCTGGGA18570.16663092372036514No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC18320.1643876425717334No Hit
GTAGTAGACGGCCGTGTCCGCAGGGGTCACAGAGGTCAGCTTCAGGGAGAACTGGTTCCTGGACATTTCCACTG17510.1571194116501666No Hit
CAGTAATAAATACCAACATCCTCAGCCTCCAGTCTGCTGATTTTCAGTGAAAAATTTGTGCCTGATCCACTGCC17440.15649129292854969No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA17250.15478639925558957No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATGCAGTGGGGATATTGTGATG17240.15469666800964432No Hit
ATTTATTACTGCATGCAAGCTCTACAAAGCCCATTCACCTTCGGCCCTGGGACCAAAGTGGAAATCATACGA16810.15083822443399772No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATGCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC16750.1502998369583261No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC16700.14985118072859976No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT16690.1497614494826545No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC16640.14931279325292815No Hit
GTACTTACAGAAGCCAGGGCAGTCTCCACAACTCCTGATGTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTG16630.14922306200698288No Hit
GTTGTGGAGACTGCCCTGGCTTCTGTAAGTACCAATCCAAATAGTTGTATCCATTATGATGCAGGAGGCTCTG16630.14922306200698288No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16260.14590300590700792No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC16240.1457235434151174No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16230.14563381216917212No Hit
GGATACAACTATTTGGATTGGTACTTACAGAAGCCAGGGCAGTCTCCACAACTCCTGATGTATTTGGGTTCTAAT16110.1445570372178289No Hit
CCTTCGGCCCTGGGACCAAAGTGGAAATCATACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG16030.14383918725026673No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATGCAGTGGGGATATTGTGATGACTCAGTCTC15600.13998074367462013No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATGCAGTGGGGATATTGTGATGACTCAGTCTCCACT15410.13827585000166004No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT15280.13710934380437154No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15120.1356736438692472No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC15050.13504552514763032No Hit
GCCCTGGCTTCTGTAAGTACCAATCCAAATAGTTGTATCCATTATGATGCAGGAGGCTCTGACTAGACCTGCAGG15020.13477633140979453No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG14890.13360982521250603No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14810.13289197524494387No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14670.1316357378017101No Hit
GCTCTACAAAGCCCATTCACCTTCGGCCCTGGGACCAAAGTGGAAATCATACGAACTGTGGCTGCACCAT14560.13064869409631213No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA14510.1302000378665858No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14390.12912326291524254No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14080.1263415942909392No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14050.1260724005531034No Hit
GGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGG13760.1234701944206906No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13730.12320100068285479No Hit
GCCACAGTTCGTATGATTTCCACTTTGGTCCCAGGGCCGAAGGTGAATGG13620.12221395697745681No Hit
CTCCTGATGTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTT13460.1207782570423325No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13380.12006040707477035No Hit
CTCCTGCATCATAATGGATACAACTATTTGGATTGGTACTTACAGAAGCC13350.11979121333693454No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13110.11763766343424809No Hit
CCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCATCAT13050.11709927595857647No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG13030.11691981346668592No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12930.11602250100723323No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12780.11467653231805419No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC12740.11431760733427312No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT12720.11413814484238258No Hit
AGTCAGAGCCTCCTGCATCATAATGGATACAACTATTTGGATTGGTACTTACAGAAGCCAGGGCAGTCTCCACA12610.11315110113698462No Hit
GTGAATGGGCTTTGTAGAGCTTGCATGCAGTAATAAATACCAACATCCTC12580.11288190739914881No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT12440.11162566995591504No Hit
GGCCGAAGGTGAATGGGCTTTGTAGAGCTTGCATGCAGTAATAAATACCA12330.11063862625051708No Hit
GGGTCTCTGGATGCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGG12280.11018997002079073No Hit
GCCCATTCACCTTCGGCCCTGGGACCAAAGTGGAAATCATACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC12200.10947212005322858No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT12120.10875427008566643No Hit
CTGTAAGTACCAATCCAAATAGTTGTATCCATTATGATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGC11970.10740830139648737No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC11850.10633152644514414No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT11840.1062417951991989No Hit
GTCTCCACAACTCCTGATGTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGATC11660.10462663277218402No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11580.10390878280462187No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCC11540.1035498578208408No Hit
GTCTACTACTGTGCGAGAGGACAATTTTTGGAGTGGTTACCTATCTTACCGGCGTGGAGACTTGACTCCTGGGGC11430.10256281411544284No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11400.10229362037760703No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11390.10220388913166176No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG11370.10202442663977122No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11290.10130657667220906No Hit
CAACTATTTGGATTGGTACTTACAGAAGCCAGGGCAGTCTCCACAACTCCTGATGTATTTGGGTTCTAATCGGGC11170.10022980172086583No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)