FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004717687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1812157
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT84470.4661295903169538No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT74900.41331959648087885No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC51300.2830880547325646No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG48690.26868532914090776No Hit
TCCATAGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCACAGGCAGTGTGTCTGGGACAGAATTCACT48380.2669746605840443No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG48270.26636764916064115No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG47780.26366368918366345No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG45230.24959206073204473No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43840.24192164365449573No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA40730.22475977522918822No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT39600.21852411242513758No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAATCAGAGCATTAGCACCTATTTAAGTTGGTATCAGCAGCA39260.2166478952982551No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA37940.20936375821741715No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAATCAGAGCATTAGCAC36940.20384547255011568No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTTCGAGGTATACTGAAACTCTGTTGAC36190.19970675829963958No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG35560.1962302383292397No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA34970.19297444978553183No Hit
GGTTAGGGGCTTTCCCTGGTTGCTGCTGATACCAACTTAAATAGGTGCTA34830.19220188979210961No Hit
GTCTGGGACAGAATTCACTCTCGCCATCAGCAGTCTGCAACCTGAAGATT34070.18800799268496052No Hit
GTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCACAGGCAGTGTG33640.18563512984802089No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC33530.18502811842461775No Hit
GAATTCACTCTCGCCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTTCAGTAT32160.17746806706041474No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGCTGACCCAGTCTCCATCCTCCCTGTCTGCATCTGT32060.1769162384936846No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCC31690.17487447279678306No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG31540.17404672994668785No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC30820.1700735642662308No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT30660.16919063855946256No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30080.16599003287242772No Hit
GTGGATGCACTATGGATGAGGAGGTTAGGGGCTTTCCCTGGTTGCTGCTGATACCAACTTAAATAGGTGCTAATG29970.16538302144902456No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT29660.1636723528921611No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTTCGAGGTAT29470.16262387861537383No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC29220.16124430719854846No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC28810.15898181007495488No Hit
CATCCATAGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCACAGGCAGTGTGTCTGGGACAGAATTC28360.15649858152466922No Hit
CACTATGGATGAGGAGGTTAGGGGCTTTCCCTGGTTGCTGCTGATACCAA27390.15114584442738682No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGCGAGAGTGA26620.1468967644635647No Hit
ACTTACTACTGTCAACAGAGTTTCAGTATACCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGA26160.14435835305660602No Hit
CCCCTAACCTCCTCATCCATAGTGCATCCACTTTGCAAAGTGGGGTCCCA25970.14330987877981874No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG25670.1416543930796283No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT24970.1377915931125173No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC24830.13701903311909508No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT24720.13641202169569194No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAATCAGA24180.13343214743534915No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23930.13205257601852377No Hit
GTTCACAGGCAGTGTGTCTGGGACAGAATTCACTCTCGCCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTT23650.13050745603167938No Hit
GTCCCAGACACACTGCCTGTGAACCTTGATGGGACCCCACTTTGCAAAGTGGATGCACTATGGATGAGGAGGTT23620.13034190746166033No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC23600.1302315417483143No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCC23550.12995562746494924No Hit
CTATGGATGAGGAGGTTAGGGGCTTTCCCTGGTTGCTGCTGATACCAACT23330.1287416046181429No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC23300.12857605604812386No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22830.12598246178449218No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22440.12383033037424462No Hit
GTAATAGACAGCCGTGTCTTCAGATCTCAGACTGCTCAGTTCCATGTAGGCCGTGGTCGCGGATGTGTTCCTGGT22340.12327850180751448No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT22190.12245075895741925No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGCTGACCCAGTCTCCATCCTCC21610.1192501532703844No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT21510.11869832470365427No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT21350.11781539899688603No Hit
GTCTATTACTGTGCGAGAGAAGTGTGGGAAAAAGGCAACTGGTTCGACCC21250.1172635704301559No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20620.11378705045975597No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTTCAGTATACCTCGAACGTTCGGCCAAGGG20610.11373186760308296No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC20470.11295930760966075No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCC20370.11240747904293061No Hit
GTGTGTCTGGGACAGAATTCACTCTCGCCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAC20300.1120211990462195No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA20130.11108309048277826No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCC20100.11091754191275922No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19730.10887577621585767No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCC18880.10418523339865145No Hit
GTATACCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACT18750.10346785626190226No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)