FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004717688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1812157
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT79510.4387588934071386No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT62950.34737608275662646No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54460.3005258374412372No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC48770.2691267919942919No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG47410.26162192348676194No Hit
TCCATAGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCACAGGCAGTGTGTCTGGGACAGAATTCACT46120.2545033349759431No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA45950.2535652264125018No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT42910.2367896379839054No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42670.23546524942375302No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG40170.22166953525549937No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT37640.20770827251722668No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAATCAGAGCATTAGCACCTATTTAAGTTGGTATCAGCAGCA35180.19413328977566513No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAATCAGAGCATTAGCAC34060.1879528098282875No Hit
GGTTAGGGGCTTTCCCTGGTTGCTGCTGATACCAACTTAAATAGGTGCTA33540.18508330128129075No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG33500.1848625698545987No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA33440.1845314727145606No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTTCGAGGTATACTGAAACTCTGTTGAC33040.18232415844764002No Hit
GTCTGGGACAGAATTCACTCTCGCCATCAGCAGTCTGCAACCTGAAGATT32970.18193787845092893No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGCTGACCCAGTCTCCATCCTCCCTGTCTGCATCTGT31560.17415709566003387No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC31030.1712324042563641No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC30980.17095648997299903No Hit
GTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCACAGGCAGTGTG30450.16803179856932926No Hit
GTGGATGCACTATGGATGAGGAGGTTAGGGGCTTTCCCTGGTTGCTGCTGATACCAACTTAAATAGGTGCTAATG30200.16665222715250388No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30130.1662659471557928No Hit
GAATTCACTCTCGCCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTTCAGTAT30100.16610039858577375No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG30060.1658796671590817No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC29900.16499674145231347No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC28810.15898181007495488No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTTCGAGGTAT27950.1542360844010756No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC27830.15357389012099945No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT27650.1525805987008852No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT27150.14982145586723447No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCC27000.14899371301713926No Hit
CATCCATAGTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCACAGGCAGTGTGTCTGGGACAGAATTC26850.14816597016704403No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26340.14535164447672028No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT26120.14413762162991398No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGCGAGAGTGA25980.14336506163649176No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA25960.14325469592314574No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC25840.14259250164306955No Hit
ACTTACTACTGTCAACAGAGTTTCAGTATACCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGA25480.14060591880284104No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAATCAGA24800.13685348454907606No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24610.13580501027228878No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT24190.13348733029202214No Hit
CCCCTAACCTCCTCATCCATAGTGCATCCACTTTGCAAAGTGGGGTCCCA24150.1332665988653301No Hit
GTCCCAGACACACTGCCTGTGAACCTTGATGGGACCCCACTTTGCAAAGTGGATGCACTATGGATGAGGAGGTT23850.13161111316513965No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT23840.13155593030846666No Hit
CACTATGGATGAGGAGGTTAGGGGCTTTCCCTGGTTGCTGCTGATACCAA23690.13072818745837142No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCC22730.12543063321776204No Hit
GTTCACAGGCAGTGTGTCTGGGACAGAATTCACTCTCGCCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTT22630.12487880465103188No Hit
CTATGGATGAGGAGGTTAGGGGCTTTCCCTGGTTGCTGCTGATACCAACT21840.12051935897386375No Hit
GTAATAGACAGCCGTGTCTTCAGATCTCAGACTGCTCAGTTCCATGTAGGCCGTGGTCGCGGATGTGTTCCTGGT21390.11803613042357809No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT21310.11759466757019398No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21180.11687729043344477No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC20680.11411814759979405No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC20510.1131800390363528No Hit
GTGTGTCTGGGACAGAATTCACTCTCGCCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAC20500.1131248561796798No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC20460.11290412475298774No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20450.11284894189631473No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCC20270.11185565047620047No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGCTGACCCAGTCTCCATCCTCC20210.11152455333616237No Hit
GTGGGGTCCCATCAAGGTTCACAGGCAGTGTGTCTGGGACAGAATTCACTCTCGCCATCAGCAGTCTGCAACCTG19650.10843431336247356No Hit
GTCTATTACTGTGCGAGAGAAGTGTGGGAAAAAGGCAACTGGTTCGACCC19480.10749620479903231No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTTCAGTATACCTCGAACGTTCGGCCAAGGG19330.10666846194893709No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCC19280.10639254766557202No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19180.10584071909884188No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCC18450.10181237056171183No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC18170.1002672505748674No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)