FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1408008
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA67470.479187618252169No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCACCTTCCAGGAACAGC65540.46548030977096716No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATACTCACGCAGGCTGTTGTCATAGGACTGGCAGAAAT63910.4539036710018693No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT62650.44495485821103287No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG57800.41050903119868637No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC54990.3905517582286464No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATACTCACGCAGGCTGTTGTCATAGGACTGGCAGAAATA52920.37585013721512944No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC51550.36612007886318826No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC48810.3466599621593059No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC47780.33934466281441583No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC46640.33124811790842096No Hit
GATGTACACTGGTACCAGCACCTTCCAGGAACAGCCCCCAAAGTCTTCGTCTATAATAACAGGAATCGGCCCTC41120.29204379520570906No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATACTCACGCAGGCTGTTGTCATAGG39980.28394725029971424No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTC39240.27869159834319124No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG38990.27691604024977134No Hit
TCCAGGAACAGCCCCCAAAGTCTTCGTCTATAATAACAGGAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG38840.27585070539371936No Hit
GGTTATGATGTACACTGGTACCAGCACCTTCCAGGAACAGCCCCCAAAGTCTTCGTCTATAATAACAGGAATCGG38100.27059505343719636No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGT34110.2422571462662144No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTTCTGCCAGT33590.23856398543190094No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATACTCACGC33300.23650433804353385No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG32520.2309645967920637No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTC31980.22712939131027662No Hit
GTCCTATGACAACAGCCTGCGTGAGTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGC31270.22208680632496408No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGG30210.21455844000886357No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT30060.21349310515281164No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT29970.21285390423918046No Hit
GTAATATACAGCCGTGTCCTCGGCCCTCAGGCTGTTCATTTCCAGATAGAGCGTGTTCTTAGAATTGTCTCTGG29500.20951585502355102No Hit
GTTCCTGGAAGGTGCTGGTACCAGTGTACATCATAACCTGCCCCGATGTT28700.20383406912460725No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATTC28360.20141931011755615No Hit
GTCATAGGACTGGCAGAAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCC27600.19602161351355957No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACC27420.19474321168629724No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG26070.18515519798182964No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTG25430.18060976926267466No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTG25360.18011261299651707No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG24890.1767745637808876No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTTCTGCCAGTCCTAT24750.17578025124857247No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT24490.17393367083141573No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGG24330.17279731365162698No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGA24260.17230015738546942No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGG23650.16796779563752479No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC23640.16789677331378797No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG23570.16739961704763043No Hit
GGATGAGGCTGATTATTTCTGCCAGTCCTATGACAACAGCCTGCGTGAGTATGTCTTCGGAACTGGGACCAAGGT23490.16683143845773604No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC22570.16029738467395072No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATG22030.15646217919216368No Hit
GTATATTACTGTGCGAGATATACCCCGGATGGGGAGACCTACGGTATGGA21720.15426048715632298No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT21520.15284004068158705No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC20860.14815256731495843No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC20330.1443883841569082No Hit
GTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGT19730.14012704473270038No Hit
GCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC19630.1394168214953324No Hit
GTACCAGCACCTTCCAGGAACAGCCCCCAAAGTCTTCGTCTATAATAACAGGAATCGGCCCTCAGGGGTCCCTG19560.13891966522917482No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG19540.13877762058170123No Hit
TCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC18970.1347293481287038No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT18640.13238561144538952No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT18560.13181743285549513No Hit
GTGTACATCATAACCTGCCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCC18520.13153334356054797No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACA18490.13132027658933756No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC18400.1306810756757064No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCC18140.12883449525854967No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC18050.1281952943449185No Hit
GCTCCAGGCTGAGGATGAGGCTGATTATTTCTGCCAGTCCTATGACAACAGCCTGCGTGAGTATGTCTTCGGA17990.1277691604024977No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT17910.12720098181260334No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC17850.12677484787018256No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT17810.12649075857523537No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCC17750.1260646246328146No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT17580.12485724512928903No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCC17400.1235788433020267No Hit
GGCTGTTGTCATAGGACTGGCAGAAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTG17340.12315270935960591No Hit
GTGCTGGTACCAGTGTACATCATAACCTGCCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGATGGTGACCCTCTG17310.12293964238839553No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC17110.12151919591365959No Hit
GTTATGATGTACACTGGTACCAGCACCTTCCAGGAACAGCCCCCAAAGTCTTCGTCTATAATAACAGGAATCGGC16620.11803910205055654No Hit
CCCCTGAGGGCCGATTCCTGTTATTATAGACGAAGACTTTGGGGGCTGTTCCTGGAAGGTGCTGGTACCAGTGT16420.1166186555758206No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG16420.1166186555758206No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATT16250.11541127607229504No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAG16090.11427491889250628No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT15930.11313856171271755No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCC15710.111576070590508No Hit
CCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGG15710.111576070590508No Hit
GTTGTCATAGGACTGGCAGAAATAATCAGCCTCATCCTCAGCCTGGAGCC15700.1115050482667712No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG15580.11065278038192965No Hit
GCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAAT15530.11029766876324568No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC15320.10880619996477293No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC15220.10809597672740497No Hit
GTCAGGGACCCCTGAGGGCCGATTCCTGTTATTATAGACGAAGACTTTGGGGGCTGTTCCTGGAAGGTGCTGGT15180.10781188743245777No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTG14970.10632041863398503No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAA14940.10610735166277464No Hit
ATAATAACAGGAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG14900.10582326236782745No Hit
CCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGT14900.10582326236782745No Hit
GGCTGAGGATGAGGCTGATTATTTCTGCCAGTCCTATGACAACAGCCTGCGTGAGTATGTCTTCGGAACTGGGAC14780.10497099448298589No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG14750.10475792751177551No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGA14720.10454486054056512No Hit
ACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG14710.1044738382168283No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC14540.10326645871330277No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGG14270.10134885597240925No Hit
CATCATAACCTGCCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGAC14160.10056761041130448No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)