FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1408008
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCACCTTCCAGGAACAGC63180.4487190413690831No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT56680.40255453094016513No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA55010.39069380287612004No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC50870.3612905608490861No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATACTCACGCAGGCTGTTGTCATAGGACTGGCAGAAAT50240.3568161544536679No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC45850.325637354333214No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG45470.3229385060312157No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG45010.3196714791393231No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC44060.31292435838432736No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC42930.30489883580206933No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATACTCACGCAGGCTGTTGTCATAGGACTGGCAGAAATA41090.2918307282344987No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC40270.2860068976880813No Hit
GATGTACACTGGTACCAGCACCTTCCAGGAACAGCCCCCAAAGTCTTCGTCTATAATAACAGGAATCGGCCCTC39740.2822427145300311No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37310.2649842898619894No Hit
GGTTATGATGTACACTGGTACCAGCACCTTCCAGGAACAGCCCCCAAAGTCTTCGTCTATAATAACAGGAATCGG36850.2617172629700968No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTC36290.25774001284083614No Hit
TCCAGGAACAGCCCCCAAAGTCTTCGTCTATAATAACAGGAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG35850.25461503059641705No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGT34560.24545315083437025No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATACTCACGCAGGCTGTTGTCATAGG33400.2372145612809018No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG33390.237143538957165No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCC31710.22521178856938312No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTTCTGCCAGT31490.2236492974471736No Hit
GTCCTATGACAACAGCCTGCGTGAGTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGC31410.22308111885727921No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTC31000.22016920358407052No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG29200.2073851853114471No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT28660.20354997982966008No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT28230.20049601990897778No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATACTCACGC28060.19928864040545227No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT27690.19666081442719077No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC27530.195524457247402No Hit
GTAATATACAGCCGTGTCCTCGGCCCTCAGGCTGTTCATTTCCAGATAGAGCGTGTTCTTAGAATTGTCTCTGG27110.19254151965045654No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGG26920.1911920954994574No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACC25160.17869216652178113No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG25030.1777688763132028No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTTCTGCCAGTCCTAT24330.17279731365162698No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGA23570.16739961704763043No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTG23220.1649138357168425No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT23100.16406156783200096No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC22850.16228600973858104No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGG22820.16207294276737064No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG22380.15894796052295157No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT21500.15269799603411344No Hit
GTCATAGGACTGGCAGAAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCC21470.15248492906290306No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATTC21470.15248492906290306No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATG21440.15227186209169266No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC21250.15092243794069352No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTG21250.15092243794069352No Hit
GGATGAGGCTGATTATTTCTGCCAGTCCTATGACAACAGCCTGCGTGAGTATGTCTTCGGAACTGGGACCAAGGT21030.14935994681848397No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGG20500.14559576366043375No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT20280.14403327253822423No Hit
GTTCCTGGAAGGTGCTGGTACCAGTGTACATCATAACCTGCCCCGATGTT20110.14282589303469867No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG20050.14239975909227787No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC19840.14090829029380514No Hit
GTATATTACTGTGCGAGATATACCCCGGATGGGGAGACCTACGGTATGGA19800.14062420099885797No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC19730.14012704473270038No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT19360.13749921875443888No Hit
GTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGC19220.13650490622212375No Hit
GTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGT19200.13636286157465016No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC18770.13330890165396786No Hit
GGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGC18630.13231458912165273No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACA18350.1303259640570224No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT18340.1302549417332856No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG18070.12833733899239208No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC17570.12478622280555224No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC17520.12443111118686827No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT17470.12407599956818427No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCC17100.1214481735899228No Hit
GTACCAGCACCTTCCAGGAACAGCCCCCAAAGTCTTCGTCTATAATAACAGGAATCGGCCCTCAGGGGTCCCTG17070.1212351066187124No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTG16980.12059590570508122No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC16600.11789705740308293No Hit
CTCACTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCA16530.11739990113692536No Hit
GGCTGAGGATGAGGCTGATTATTTCTGCCAGTCCTATGACAACAGCCTGCGTGAGTATGTCTTCGGAACTGGGAC16460.11690274487076778No Hit
GCTCCAGGCTGAGGATGAGGCTGATTATTTCTGCCAGTCCTATGACAACAGCCTGCGTGAGTATGTCTTCGGA16430.11668967789955739No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCC16170.11484309748240067No Hit
GTTATGATGTACACTGGTACCAGCACCTTCCAGGAACAGCCCCCAAAGTCTTCGTCTATAATAACAGGAATCGGC15950.11328060636019113No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG15850.11257038312282316No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCC15620.11093686967687684No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT15450.10972949017335129No Hit
CCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGT15090.1071726865188266No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15080.10710166419508979No Hit
GGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTC14850.10546815074914348No Hit
GGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCACCTTC14760.1048289498355123No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC14730.10461588286430192No Hit
GCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC14670.10418974892188113No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC14660.10411872659814433No Hit
CCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGG14610.10376361497946034No Hit
CCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTTCTGCCAGTCCTATGACAAC14560.10340850336077637No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCC14550.10333748103703956No Hit
GGCTGTTGTCATAGGACTGGCAGAAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTG14530.10319543638956598No Hit
ATAATAACAGGAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG14390.1022011238572508No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGA14330.10177498991483004No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG14320.10170396759109322No Hit
GTGCTGGTACCAGTGTACATCATAACCTGCCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGATGGTGACCCTCTG14190.10078067738251487No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC14160.10056761041130448No Hit
ATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTG14100.10014147646888369No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)