FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004717697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1932619
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG69440.3593051708588191No Hit
GATATATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACGCTATTGCT64410.33327831300427035No Hit
GTATATATCTCCACCACTTCCACTAATAGTTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCT61610.3187902012760922No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGC54090.27987927263469936No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG53830.27853394797422565No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49830.25783664550539964No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCG49400.25561168549000085No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCCATTTTAAAAGGTGTCC48460.25074781940982677No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT43270.22389306945652504No Hit
ATATAAGGCCGTGTCCTCGGCTCTCAGGCTGTTCACTTGTAGCAATAGCG41880.216700756848608No Hit
GTAATATAAGGCCGTGTCCTCGGCTCTCAGGCTGTTCACTTGTAGCAATAGCGTGTTCTTGGAATTGTCTCTGG41840.21649378382391976No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC37350.1932610618026626No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCATCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG35400.18317112684910994No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGC35000.18110139660222735No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCTTGTATTTCCAGGAGAAAGTGATGG31940.16526796021357545No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC31240.16164593228153093No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT30560.1581273908618305No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT29800.15419490339275357No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29760.1539879303680653No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC29200.15109030802242968No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC29190.1510385647662576No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG28760.1488136047508588No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC28740.14871011823851468No Hit
GCCTTATATTACTGCGCGAAAAAAAGGAGTAGCTGGCCTCCTGAGTTTGACTCCTGGGGCCAGGGAACCCTGGTC28460.14726130706569684No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCTGAACCTGCGGGAGTCGGCCACCATC28120.14550203635584666No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT28040.14508809030647013No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGAGTCAAACTCAG27410.14182826516763006No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27330.14141431911825353No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCT26590.1375853181615207No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCGGGGATGCCAGTGGCCCTGTT26010.13458420930354095No Hit
GCCCTGGGATTTCCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTG25970.1343772362788527No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG25910.1340667767418203No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAG25080.1297720864795389No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24920.12894419438078586No Hit
CAGTAATATAAGGCCGTGTCCTCGGCTCTCAGGCTGTTCACTTGTAGCAA24840.12853024833140936No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT24750.12806455902586075No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGGCATAGATGCTAAAGGTGAATCCAGAGGCTGCAC23770.12299371992099839No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAGCTGGCCTCTCACTTTCGGCGGAGGG23660.12242454410310569No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA23370.12092398967411581No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC23050.11926820547660973No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCCATTTTAAAAGGTGTCCAGTGTGAGGTGCAAATATTGGAAT22710.1175089347667595No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22500.11642232638714614No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT22210.11492177195815627No Hit
GCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGGTTG22130.11450782590877974No Hit
TTTTAAAAGGTGTCCAGTGTGAGGTGCAAATATTGGAATCTGGGGGAGGCTTGGTACAGCCGGGGGGGTCCCTG22110.11440433939643561No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCACGGCG22070.11419736637174735No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC22000.1138351635785429No Hit
GGGTTCCCTGGCCCCAGGAGTCAAACTCAGGAGGCCAGCTACTCCTTTTTTTCGCGCAGTAATATAAGGCCGTGT21960.11362819055385463No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGT21690.11223112263720889No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCATCTATGCC21470.11109277100142345No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG21300.11021313564649834No Hit
CCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAGCCAGAGCATGTTCACCTGCCGCGTGG21270.11005790587798214No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC20550.10633239143359348No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC20550.10633239143359348No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT20420.10565972910335664No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT20270.10488358026077567No Hit
CCTTTAGCATCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG20110.10405568816202262No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT19960.10327953931944164No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA19690.1018824714027959No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACACACATGGAGGACGCATTCTGCTGGA19610.10146852535341938No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19610.10146852535341938No Hit
GGTCTGTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCC19570.10126155232873112No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGTGAGAGGCCAGCTGCTACGCTGCTGAC19470.10074411976701046No Hit
GAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTCCAGGTGTTTTCATTTGGTGATCAGGACTGAAC19360.10017494394911775No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)