FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989255
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40250.4068718378982163No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT37480.3788709685571465No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA27620.27920000404344686No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG27360.2765717635998807No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27270.2756619880617232No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT25600.2587805975203562No Hit
GTCTGTATCTGTAGGAGACAGAGTCACCATCACCTGCCAGGCGAGTCAGG23900.24159594846627006No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG22660.2290612632738778No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22290.2253210749503414No Hit
GATCTACGCTGCATCCAATTTGGAAACAGGGGTCCCGTCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTT22180.22420912707037116No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22000.22238957599405615No Hit
ATATTACTGTCAACAGTTTGATAATCTGATCACCTTCGGCCAAGGGACACGACTGGAGATTAGACGAACTGTGG20400.2062157886490339No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20260.20480058225634443No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC19770.19984735988193134No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG18840.19044634598763716No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC18620.1882224502276966No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18140.1833703140241899No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG18070.1826627108278452No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17630.17821491930796407No Hit
GTCTCCATCCTCCCTGTCTGTATCTGTAGGAGACAGAGTCACCATCACCTGCCAGGCGAGTCAGGACATTAGCA17510.1770018852570874No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17340.1752834203516788No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16850.17033019797726573No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCC16780.169622594780921No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA16750.16931933626820184No Hit
GTAGGAGACAGAGTCACCATCACCTGCCAGGCGAGTCAGGACATTAGCAACTATTTAAATTGGTTTCAGCAGAA16230.1640628553810696No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC16190.16365851069744405No Hit
AGTCAGGACATTAGCAACTATTTAAATTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTACG15670.1584020298103118No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCC15530.15698682341762235No Hit
ACATATTACTGTCAACAGTTTGATAATCTGATCACCTTCGGCCAAGGGAC15460.15627922022127763No Hit
GTGCAGCCACAGTTCGTCTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATCAGATTATCAAACTGTTGACAGT14370.1452608275924812No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCC14260.14414887971251092No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC14100.1425315009780087No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCC14100.1425315009780087No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14050.14202607012347676No Hit
GCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAATT13960.14111629458531924No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC13930.14081303607260007No Hit
CTCCTGTACAGCTTCTGGATTCACCTTTGGTGATTATGCTATGACCTGGGTCCGCCAGGCTCCAGGGAAGGG13800.13949891585081703No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13530.13676958923634452No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG12920.1306033328110548No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT12640.1277729200256759No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATCAGATTATC12520.12655988597479922No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT12490.12625662746208005No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12020.12150557742947976No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT11950.12079797423313503No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCC11630.11756321676413058No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT11330.1145306316369389No Hit
GGACACGACTGGAGATTAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGA11230.11351976992787502No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG11200.11321651141515585No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11170.11291325290243667No Hit
CTCCTGATCTACGCTGCATCCAATTTGGAAACAGGGGTCCCGTCAAGGTT10940.11058827097158973No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC10860.10977958160433862No Hit
GTTCAGTGGAAGTGGATCTGGGACAGATTTTACTTTCACCATCAGCAGCC10840.10957740926252585No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10820.10937523692071308No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10750.10866763372436834No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10680.10796003052802362No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10650.10765677201530445No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAATTTAAAT10540.10654482413533417No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC10420.10533179008445749No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC10270.10381549752086167No Hit
GCCTGAAGATATTGCAACATATTACTGTCAACAGTTTGATAATCTGATCACCTTCGGCCAAGGGACACGACTGG10140.1025013772990786No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10120.10229920495726581No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10110.10219811878635944No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10030.10138942941910832No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT9950.10058074005185721No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCAGATTATCAAACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGC9940.10047965388095081No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT9910.10017639536823164No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC9910.10017639536823164No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)