FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1208919
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATACTGCATCCACTTTGCAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTC90470.7483545216842485No Hit
GTATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGAC74440.6157567215007788No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60490.500364375115289No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT59020.48820475151767817No Hit
CTCTTAAGCTGGTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATCGTGAGAGTGAATTCTGTC53780.44486024291122894No Hit
GCTTAAGAGTTACCCTTCCACTTTCGGCGGAGGGACCAAGGTGGAGCTCAAACGAACTGTGGCTGCACCAT47440.39241669623854036No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42190.34898946910421624No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCC37500.3101944795308867No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG37430.3096154498357624No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36520.3020880637991462No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG36000.2977867003496512No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA35350.2924099960377825No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT34790.28777775847678794No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG34680.286867854670164No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT33900.28041580949592154No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32920.27230939376418106No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGACATTAGCATTTATTTAGTCTGGTATCAGCAAAA31410.2598188960550707No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC30590.25303597676932865No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA29850.2469148057065858No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCC29050.24029732347659355No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG27470.22722779607235885No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA27130.22441536612461216No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT27110.22424992906886235No Hit
GTGCAGCCACAGTTCGTTTGAGCTCCACCTTGGTCCCTCCGCCGAAAGTGGAAGGGTAACTCTTAAGCTGGTGAC26610.2201140026751172No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25940.21457186130749867No Hit
GACCAAGGTGGAGCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA25750.21300020927787552No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25220.20861612730050566No Hit
ATACTGCATCCACTTTGCAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTC24140.19968252629001612No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT23950.19811087426039298No Hit
ACCTTGGTCCCTCCGCCGAAAGTGGAAGGGTAACTCTTAAGCTGGTGACAGTAATAAGTTGCAAAATCTTCAGG23920.19786271867676827No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23510.19447125903389723No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC23430.193809510810898No Hit
GTCCTGATCTATACTGCATCCACTTTGCAGAGTGGGGTCCCATCAAGGTT23320.19289960700427405No Hit
CCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGACTAAATAAATGCTA21790.18024367223941387No Hit
GAATTCACTCTCACGATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCACCAGCTTAAGAGTTAC21610.17875473873766565No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC21590.17858930168191584No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21290.17610774584566874No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21110.17461881234392046No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC20750.17164094534042396No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC20660.17089647858954982No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCC20430.16899395244842705No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT19700.16295549991355915No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGACTAAAT18620.1540218989030696No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18330.1516230615946974No Hit
AGTCAGGACATTAGCATTTATTTAGTCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGGTCCTGATCTAT17870.14781800931245187No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17740.14674266845007813No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT17700.1464117943385785No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17450.14434383114170596No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT17430.14417839408595615No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC17220.14244130500058316No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16360.1353275116033415No Hit
ACTTATTACTGTCACCAGCTTAAGAGTTACCCTTCCACTTTCGGCGGAGGGACCAAGGTGGAGCTCAAACGA16360.1353275116033415No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT16080.13301139282284422No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC16080.13301139282284422No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16030.1325978001834697No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC15830.13094342962597164No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTG15710.1299508072914728No Hit
GTCTCCTACAGATGCAGACAGGAAGGATGGAGACTGGGTCAACTGGATGTCACATCTGGCACCTGGGAGCCAG15130.1251531326747284No Hit
GGGTAACTCTTAAGCTGGTGACAGTAATAAGTTGCAAAATCTTCAGGCTG15080.12473954003535391No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15080.12473954003535391No Hit
ATGCAGTATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGACTAAATAAATGCTAATGTC14990.12399507328447977No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC14820.12258885831060641No Hit
CAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATCGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG14600.12076905069735855No Hit
CTGCAAAGTGGATGCAGTATAGATCAGGACCTTAGGGGCTTTCCCTGGTT14540.12027273953010914No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14380.11894924308411069No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACGATCAGCAGCCTGCAGCCTGAAGATTTTG14150.1170467169429879No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14140.116963998415113No Hit
GAGTTACCCTTCCACTTTCGGCGGAGGGACCAAGGTGGAGCTCAAACGAACTGTGGCTGCACCATCTGTCTTCAT14000.11580593902486436No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA14000.11580593902486436No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG13990.11572322049698947No Hit
CCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG13920.11514419080186514No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGCTCCACCTTGGTCCCTCCGCCGAAAGTGGAAGGGTAACT13820.1143170055231161No Hit
ACCTTGATGGGACCCCACTCTGCAAAGTGGATGCAGTATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTTTG13800.11415156846736631No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13670.11307622760499256No Hit
TTATTACTGTCACCAGCTTAAGAGTTACCCTTCCACTTTCGGCGGAGGGA13590.11241447938199332No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13430.11109098293599487No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13380.11067739029662037No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATCGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG13360.11051195324087056No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13330.11026379765724587No Hit
CCCCTAAGGTCCTGATCTATACTGCATCCACTTTGCAGAGTGGGGTCCCA13290.10993292354574624No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC13100.10836127151612308No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTC13080.10819583446037327No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACGATCAGCAGCCTGCAGCCTG12980.10736864918162424No Hit
GCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTCCTGTCTGCATCTGTAGG12770.10563156009625127No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12690.10496981187325205No Hit
GAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACGATCAGCAGCCTGCAGC12550.10381175248300341No Hit
ATCTGGGACAGAATTCACTCTCACGATCAGCAGCCTGCAGCCTGAAGATT12520.10356359689937869No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTCCTGTCTGCATCTGT12490.103315441315754No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12410.10265369309275477No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC12310.10182650781400573No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG12310.10182650781400573No Hit
GTCCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGAAGGATGGAGACTGGGTCA12230.1011647595910065No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12230.1011647595910065No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12200.10091660400738181No Hit
GAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCT12180.100751166951632No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)