FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1208919
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATACTGCATCCACTTTGCAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTC81710.6758930912658333No Hit
GTATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGAC66630.5511535512304795No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT61760.5108696281554016No Hit
CTCTTAAGCTGGTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATCGTGAGAGTGAATTCTGTC56810.4699239568573246No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT50030.4138407949581403No Hit
GCTTAAGAGTTACCCTTCCACTTTCGGCGGAGGGACCAAGGTGGAGCTCAAACGAACTGTGGCTGCACCAT47070.38935611070716897No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39590.32748265185674147No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA37610.3111043833375106No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCC35060.2900111587294103No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33410.2763626016300513No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33370.2760317275185517No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT33320.2756181348791772No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG32680.2703241490951834No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG32510.26891793412131004No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT29750.24608762042783675No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28370.2346724635811001No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC27810.23004022602010557No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCC27500.22747595165598355No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG27400.22664876637723455No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA27320.2259870181542353No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGACATTAGCATTTATTTAGTCTGGTATCAGCAAAA26890.22243012145561447No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25690.2125038981106261No Hit
GTGCAGCCACAGTTCGTTTGAGCTCCACCTTGGTCCCTCCGCCGAAAGTGGAAGGGTAACTCTTAAGCTGGTGAC25160.20811981613325625No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC25060.2072926308545072No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG24640.20381845268376128No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24130.1995998077621412No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT24090.19926893365064163No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23930.19794543720464317No Hit
ACCTTGGTCCCTCCGCCGAAAGTGGAAGGGTAACTCTTAAGCTGGTGACAGTAATAAGTTGCAAAATCTTCAGG23550.19480213314539682No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT23390.19347863669939838No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC22280.18429688010528414No Hit
GTCCTGATCTATACTGCATCCACTTTGCAGAGTGGGGTCCCATCAAGGTT21900.18115357604603782No Hit
ATACTGCATCCACTTTGCAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTC21700.17949920548853976No Hit
GACCAAGGTGGAGCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA21700.17949920548853976No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA21600.17867202020979073No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21400.17701764965229266No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC21010.17379162706517146No Hit
GAATTCACTCTCACGATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCACCAGCTTAAGAGTTAC20520.1697384191993012No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20390.16866307833692745No Hit
CCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGACTAAATAAATGCTA20340.16824948569755294No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT19620.1622937516905599No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC19150.15840598088043947No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCC18550.15344286920794528No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC17810.14732169814520246No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGACTAAAT17690.1463290758107036No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17220.14244130500058316No Hit
GGGTAACTCTTAAGCTGGTGACAGTAATAAGTTGCAAAATCTTCAGGCTG17050.14103509002670983No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT16560.13698188216083956No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC16330.1350793560197168No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15760.1303643999308473No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC15700.1298680887635979No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC15680.1297026517078481No Hit
ACTTATTACTGTCACCAGCTTAAGAGTTACCCTTCCACTTTCGGCGGAGGGACCAAGGTGGAGCTCAAACGA15670.1296199331799732No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACGATCAGCAGCCTGCAGCCTG15610.12912362201272376No Hit
CTGCAAAGTGGATGCAGTATAGATCAGGACCTTAGGGGCTTTCCCTGGTT15410.1274692514552257No Hit
AGTCAGGACATTAGCATTTATTTAGTCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGGTCCTGATCTAT15280.12639391059285196No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG15190.12564944384197782No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15120.12507041414685352No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14830.12267157683848132No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTG14400.1191146801398605No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14380.11894924308411069No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA14370.11886652455623578No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC14320.11845293191686126No Hit
CAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATCGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG14270.11803933927748674No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14250.11787390222173695No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14190.11737759105448752No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14150.1170467169429879No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14080.11646768724786359No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGCTCCACCTTGGTCCCTCCGCCGAAAGTGGAAGGGTAACT13950.11539234638548986No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13880.11481331669036551No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATCGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG13780.1139861314116165No Hit
GTCTCCTACAGATGCAGACAGGAAGGATGGAGACTGGGTCAACTGGATGTCACATCTGGCACCTGGGAGCCAG13540.11200088674261882No Hit
ATGCAGTATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGACTAAATAAATGCTAATGTC13190.10910573826699722No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13150.1087748641554976No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13070.10811311593249837No Hit
GAGTTACCCTTCCACTTTCGGCGGAGGGACCAAGGTGGAGCTCAAACGAACTGTGGCTGCACCATCTGTCTTCAT12770.10563156009625127No Hit
CCCCTAAGGTCCTGATCTATACTGCATCCACTTTGCAGAGTGGGGTCCCA12500.1033981598436289No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT12440.10290184867637947No Hit
TTATTACTGTCACCAGCTTAAGAGTTACCCTTCCACTTTCGGCGGAGGGA12180.100751166951632No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC12160.10058572989588219No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)