FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1165857
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44270.3797206690014298No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT41380.3549320371194752No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC34560.2964342968305719No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29430.25243233089478384No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27650.23716459222700553No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG26880.23056000864600032No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAATCAGAGTGTCAGCGG25550.21915209155153678No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24730.21211863890682991No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA23410.20079649562510668No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG23370.20045340037414539No Hit
CATCTATGGTGCATCCACCCGGGCCACTGGCGTCCCAGACAGGTTCTCTGGCAGTGGGTCGGGGACAGACTTC23180.19882369793207916No Hit
CTCCTGCAGGGCCAATCAGAGTGTCAGCGGCAACTACTTAGCCTGGTATC22700.19470655492054345No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT21610.1853572093318477No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20710.17763756618521825No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG20580.176522506619594No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTCTCTGCTGATACCAGGC18710.16048280363715275No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAAGGCCCACTGCTGACAGTAATAAACTGCAA18620.1597108393224898No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC18610.15962506550974948No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA18300.1569660773147993No Hit
GTTTATTACTGTCAGCAGTGGGCCTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCAT17070.14641589834773905No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGATTTTGCAGTTTATTACTGTCAGCAGTGGGCCTTCGGC17000.14581548165855676No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16990.14572970784581643No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16990.14572970784581643No Hit
GTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAATCAGA16960.14547238640759544No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC16950.1453866125948551No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC16770.1438426839655292No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16560.14204143389798235No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAAGGCCCACTGCTGAC16550.14195566008524202No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16320.13998286239221447No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC15930.1366376836953417No Hit
GTCTGAGACTTGAGGACACGGCCTTGTATTACTGTGCCAGAGGACGGACGTACTACTACGACGCTAGTGG15890.1362945884443804No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT15520.1331209573729883No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15130.1297757786761155No Hit
CAGTAATACAAGGCCGTGTCCTCAAGTCTCAGACTGTTCATTTGCAGATA14860.1274598857321267No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGGG14210.12188458790400537No Hit
GGCCACTGGCGTCCCAGACAGGTTCTCTGGCAGTGGGTCGGGGACAGACTTCACTCTCACCATCAGCAGACTGG14160.12145571884030373No Hit
GCACTGAACACAGAGGGCTCACCATGGACTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTTAAAAGATGTCC13960.11974024258549719No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC13870.11896827827083424No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT13860.11888250445809391No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC13810.11845363539439227No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT13620.11682393295232606No Hit
CTCCTGTGCAGCCTCTGGATTCACATTTGATGATTATGCCATGCACTGGGTGCGGCAAGTTCCAGGGAAGGGC13610.11673815913958573No Hit
CTATGCGGACTCTGTGAAGGGCCGATTCAGCATTTCCAGAGACAACGCCAATAACTCCCTGTATCTGCAAATGA13490.1157088733867018No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13460.11545155194848081No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAATCAGAGTGTCAGCGGCA13230.1134787542554533No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13160.11287833756627098No Hit
GTGTTGACACAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAATC13120.11253524231530967No Hit
GTATTACTGTGCCAGAGGACGGACGTACTACTACGACGCTAGTGGAGACTGGTTCGACTCCTGGGGCCAGGGA13070.11210637325160804No Hit
GTCTCAGACTGTTCATTTGCAGATACAGGGAGTTATTGGCGTTGTCTCTGGAAATGCTGAATCGGCCCTTCAC13050.1119348256261274No Hit
GAGTGAAGTCTGTCCCCGACCCACTGCCAGAGAACCTGTCTGGGACGCCAGTGGCCCGGGTGGATGCACCAT12960.11116286131146445No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12920.11081976606050314No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC12790.10970470649487889No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12760.10944738505665788No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG12690.10884696836747561No Hit
GTAATAAACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCCGACCCACTGCCAG12640.10841809930377397No Hit
GCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCCGGGCCACTGGCGTCCCAGACAGGTT12430.1066168492362271No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTCTCCAGGCACCCTGTCTTTGTCTCC12400.10635952779800609No Hit
GTAATACAAGGCCGTGTCCTCAAGTCTCAGACTGTTCATTTGCAGATACAGGGAGTTATTGGCGTTGTCTCTGGA12300.10550178967060284No Hit
GCCATGCACTGGGTGCGGCAAGTTCCAGGGAAGGGCCTGGAGTGGATCTCAGTTATTAATTGGGATAGTGGGAGG12260.10515869441964153No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12150.10421518247949792No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12140.10412940866675757No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT12120.10395786104127694No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12110.10387208722853661No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT12100.10378631341579628No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12030.10318589672661399No Hit
CTACTTAGCCTGGTATCAGCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCCGGGCC11990.10284280147565268No Hit
TCTATGGTGCATCCACCCGGGCCACTGGCGTCCCAGACAGGTTCTCTGGCAGTGGGTCGGGGACAGACTTCACT11900.10207083716098973No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11830.10147042047180743No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC11810.10129887284632678No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11770.10095577759536548No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)