FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1165857
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44660.3830658476983026No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT37420.3209656072743055No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31560.2707021530084736No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC30530.26186745029621983No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27280.2339909611556134No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24710.21194709128134923No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA24570.21074625790298468No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23900.20499941244938275No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAATCAGAGTGTCAGCGG23140.19848060268111783No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG22950.1968509002390516No Hit
CATCTATGGTGCATCCACCCGGGCCACTGGCGTCCCAGACAGGTTCTCTGGCAGTGGGTCGGGGACAGACTTC21840.18733000702487526No Hit
CTCCTGCAGGGCCAATCAGAGTGTCAGCGGCAACTACTTAGCCTGGTATC21710.18621494745925102No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT21500.18441369739170413No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19290.16545768477609174No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAAGGCCCACTGCTGACAGTAATAAACTGCAA18360.1574807201912413No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG18060.15490750580903148No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17380.1490748865426892No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17300.14838869604076657No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC16910.1450435173438938No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG16790.14401423159100987No Hit
GTTTATTACTGTCAGCAGTGGGCCTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCAT16750.14367113634004858No Hit
GTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAATCAGA16700.14324226727634692No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC16380.14049750526865645No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTCTCTGCTGATACCAGGC16240.13929667189029185No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGATTTTGCAGTTTATTACTGTCAGCAGTGGGCCTTCGGC16170.13869625520110956No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC16020.13740964801000466No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15990.13715232657178367No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC15880.13620881463164006No Hit
GTCTGAGACTTGAGGACACGGCCTTGTATTACTGTGCCAGAGGACGGACGTACTACTACGACGCTAGTGG15320.13140548111818173No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15240.13071929061625912No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT15190.13029042155255746No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA15120.1296900048633752No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC15090.12943268342515418No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC14920.12797452860856862No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC14890.12771720717034765No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAAGGCCCACTGCTGAC14610.12531554041361848No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14510.12445780228621521No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT14240.12214190934222637No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT13560.11630929007588409No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAATCAGAGTGTCAGCGGCA13480.11562309957396147No Hit
GTCTCAGACTGTTCATTTGCAGATACAGGGAGTTATTGGCGTTGTCTCTGGAAATGCTGAATCGGCCCTTCAC13450.1153657781357405No Hit
CTCCTGTGCAGCCTCTGGATTCACATTTGATGATTATGCCATGCACTGGGTGCGGCAAGTTCCAGGGAAGGGC13370.11467958763381786No Hit
CAGTAATACAAGGCCGTGTCCTCAAGTCTCAGACTGTTCATTTGCAGATA13320.11425071857011625No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTCTCCAGGCACCCTGTCTTTGTCTCC13280.11390762331915491No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13270.11382184950641461No Hit
GGCCACTGGCGTCCCAGACAGGTTCTCTGGCAGTGGGTCGGGGACAGACTTCACTCTCACCATCAGCAGACTGG13060.1120205994388677No Hit
GTGTTGACACAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAATC12840.1101335755585805No Hit
GCACTGAACACAGAGGGCTCACCATGGACTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTTAAAAGATGTCC12540.10756036117637068No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG12440.10670262304896742No Hit
GAGTGAAGTCTGTCCCCGACCCACTGCCAGAGAACCTGTCTGGGACGCCAGTGGCCCGGGTGGATGCACCAT12440.10670262304896742No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12410.10644530161074642No Hit
CTATGCGGACTCTGTGAAGGGCCGATTCAGCATTTCCAGAGACAACGCCAATAACTCCCTGTATCTGCAAATGA12310.10558756348334314No Hit
GTATTACTGTGCCAGAGGACGGACGTACTACTACGACGCTAGTGGAGACTGGTTCGACTCCTGGGGCCAGGGA12310.10558756348334314No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12180.10447250391771888No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12120.10395786104127694No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT12010.10301434910113333No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12000.10292857528839301No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC11730.10061268234440417No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC11710.1004411347189235No Hit
GCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCCGGGCCACTGGCGTCCCAGACAGGTT11670.1000980394679622No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)