FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948612
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATCACTGTGCAGCATGGGATGACAACCTGAGTGGTTGGGTGTTCGGCAGAGGGACCAAGCTGACCGTCCT49560.5224475338705393No Hit
GTGATAATCAGCCTCATCGTCGGACCGGAGGCCACTGATGGCCAGGGTGGCAGAGGGGCCAGACTTGGAGCCAG42460.4476013375331537No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC40920.4313670921303968No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC38290.40364237433218214No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG35140.3704359632810886No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT32990.34777127002399294No Hit
GTCTATGGTGGCTCCATCATCAGTAGTAGTTACTACTGGGGCTGGATCCG32520.3428166626608139No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA31470.331747858977116No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT31460.33164244179917607No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCTGCCGAACACCCAACCACTCAGGTTGTCATCCCATG31060.32742575468157686No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCTGCCGAACACCCAACCACTCAGGTTGTCATCCCATGCTGCACAGTGAT30560.32215489578457784No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC28890.3045502270686013No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCT28840.3040231411789014No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCC26710.28156928227768574No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC26510.2794609387188861No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTC25880.2728196565086674No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25700.27092214730574776No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG24240.25553123932651073No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC23650.24931162582805194No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG23320.2458328589560326No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC22970.24214325772813333No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC22940.24182700619431338No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT22790.2402457485252137No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG22080.23276112889147513No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21760.22938777919739578No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21130.22274649698717708No Hit
GGGTGGCAGAGGGGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTGATCGTTCCTAT20760.2188460614033978No Hit
ATGCTGCACAGTGATAATCAGCCTCATCGTCGGACCGGAGGCCACTGATG20550.21663230066665823No Hit
GTGCAGCATGGGATGACAACCTGAGTGGTTGGGTGTTCGGCAGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCA20430.21536729453137848No Hit
CTATAGGAACGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCCCCTCTGCCACC20350.21452395710785865No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20220.21315353379463892No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG19790.20862059514321976No Hit
GGCCATCAGTGGCCTCCGGTCCGACGATGAGGCTGATTATCACTGTGCAG19420.2047201595594405No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC19320.20366598778004072No Hit
CCCCTGAGGGCCGCTGATCGTTCCTATAGATGAGGAGTCTGGGGGCCGTTCCTGGGAGCTGCTGATACCAATAA19060.20092514115360127No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC18630.19639220250218214No Hit
TTCCTATAGATGAGGAGTCTGGGGGCCGTTCCTGGGAGCTGCTGATACCA18420.19417844176544255No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18390.19386219023162263No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTTTGTTTATTGGTATCAGCAGCTCCCAGGAACGGCCCC18220.19207009820664298No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG17830.18795882826698376No Hit
GTCTGGGGGCCGTTCCTGGGAGCTGCTGATACCAATAAACAAAATTACTTCCGATGTTGGAGCTGCTTCCAGAAC17830.18795882826698376No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA17670.18627215341994408No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17620.1857450675302442No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA17360.18300422090380472No Hit
GCCCCCAGACTCCTCATCTATAGGAACGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT17060.17984170556560533No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC16830.1774171104729858No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG16230.17109207979658703No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16100.1697216564833673No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTT15970.16835123317014755No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCCCCTCTGCCACCCTGGCCATCAGTGGCCTCCGGT15850.16708622703486778No Hit
GCATGGGATGACAACCTGAGTGGTTGGGTGTTCGGCAGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG15770.16624288961134795No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15670.16518871783194813No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC15650.1649778834760682No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15560.16402912887460838No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT15450.1628695399172686No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC15140.15960160740112922No Hit
CTGATGGCCAGGGTGGCAGAGGGGCCAGACTTGGAGCCAGAGAATCGGTC14900.1570715951305697No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14330.15106281598799087No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC14210.14979780985271113No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG14060.1482165521836114No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCTGCCGAACACCCAACCACTCAGGTTGTCATCCCATGCTGCACAGTGATA14000.14758404911597156No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT13990.14747863193803157No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCT13890.14642446015863175No Hit
CCCAGGAACGGCCCCCAGACTCCTCATCTATAGGAACGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG13750.14494861966747205No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCTGCCGAACACCCAAC13540.14273485893073248No Hit
ATCCCATGCTGCACAGTGATAATCAGCCTCATCGTCGGACCGGAGGCCACTGATGGCCAGGGTGGCAGAGGGGCC13400.14125901843957275No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTTT13350.14073193254987287No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGG13300.14020484666017297No Hit
CTGCCACCCTGGCCATCAGTGGCCTCCGGTCCGACGATGAGGCTGATTATCACTGTGCAGCATGGGATGACAAC13020.13725316567785353No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGC12950.1365152454322737No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCCCCTCTG12600.1328256442043744No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGG12520.13198230678085457No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC12500.13177147242497458No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12490.1316660552470346No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12430.13103355217939475No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12430.13103355217939475No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT12430.13103355217939475No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACA12410.13082271782351476No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA12400.1307173006455748No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTC12230.12892520862059514No Hit
CTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTC12190.12850353990883523No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG12050.1270276994176755No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC12040.12692228223973553No Hit
GCTGATACCAATAAACAAAATTACTTCCGATGTTGGAGCTGCTTCCAGAA12000.12650061352797562No Hit
CAATAATACACAGCCGTATCTGCGGCGGTCACAGACCTCACCTTCAGGGA11650.12281101230007631No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC11640.12270559512213636No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG11510.12133517180891662No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCC11500.12122975463097663No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT11070.1166968159795575No Hit
GACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTGATCGTT11060.11659139880161751No Hit
CTCCTCATCTATAGGAACGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCCCCT11010.11606431291191763No Hit
GTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCT11010.11606431291191763No Hit
ACCATAGACAGTGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCACCAGTCCTGGGCCCGACTCCTGCAGCTGCAC11010.11606431291191763No Hit
CATCTATAGGAACGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCCCCTCTGCCAC10920.11511555831045782No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10910.11501014113251783No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCC10900.11490472395457785No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC10850.11437763806487794No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCTGCCGAACACCCAACCACTC10800.11385055217517803No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGC10770.11353430064135811No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC10680.11258554603989829No Hit
ATAATACACAGCCGTATCTGCGGCGGTCACAGACCTCACCTTCAGGGAGA10590.11163679143843847No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAG10570.11142595708255852No Hit
GTTCCTGGGAGCTGCTGATACCAATAAACAAAATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTG10540.11110970554873859No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGG10490.11058261965903868No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGC10320.10879052763405903No Hit
GGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC10100.10647134971937947No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG10080.10626051536349952No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10070.10615509818555954No Hit
GGTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG10060.10604968100761955No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC10050.10594426382967957No Hit
TCAGTGGCCTCCGGTCCGACGATGAGGCTGATTATCACTGTGCAGCATGG10040.10583884665173958No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9990.1053117607620397No Hit
TCACTGTGCAGCATGGGATGACAACCTGAGTGGTTGGGTGTTCGGCAGAGGGACCAAGCTGACCGTCCTAGGTC9960.10499550922821975No Hit
GTTCTGGAAGCAGCTCCAACATCGGAAGTAATTTTGTTTATTGGTATCAGCAGCTCCCAGGAACGGCCCCC9770.10299258284736015No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCCCCTCTGCCACCCTGGC9720.10246549695766025No Hit
ACCTACTACACCCCGTCCCTCAAGAGTCGAGTCACCATATCCGGAGACAC9700.10225466260178027No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA9690.10214924542384031No Hit
GGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTTTGTTTATTGGTATCAGC9640.10162215953414042No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9630.10151674235620042No Hit
CCCAACCACTCAGGTTGTCATCCCATGCTGCACAGTGATAATCAGCCTCATCGTCGGACCGGAGGCCACTGATGG9560.10077882211062057No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)