FastQCFastQC Report
Wed 8 May 2024
EGAF00004717717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences782712
Total Bases57.8 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43790.5594650395036744No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT42000.5365958360163126No Hit
GTATGATAATCTCCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGAGCA30230.3862212410184078No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29810.3808552826582447No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28880.36897351771788345No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG28580.36514069031776697No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG27340.349298337063952No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26990.3448267050971494No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT25560.3265568944899273No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24800.31684706507629884No Hit
TTATCATACTGTTGACAGAAATATGTTGCAATATCTTCAGGCTGCAGGCT24720.31582497776960106No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23700.3027933646092049No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAACTA22670.2896339905354715No Hit
CCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGAGCAAACGAACTGTGG22330.28529011948200617No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22320.28516235856866895No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC21360.27289731088829605No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC21080.269320005314854No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG20760.26523165608806304No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20650.26382628604135366No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20310.25948241498788827No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20080.2565439139811323No Hit
AACCAGGGAAAGCCCCTAAGCTCCTGATCTACGATACATCCAATTTGGAA20000.2555218266744345No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA19950.2548830221077485No Hit
GTGTAAGGGAGATTATCATACTGTTGACAGAAATATGTTGCAATATCTTC19560.24990034648759699No Hit
GTGCAGCCACAGTTCGTTTGCTCTCCAGCTTGGTCCCCTGGCCAAAAGTG19050.24338453990739886No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC19040.2432567789940617No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA18310.23393023232044483No Hit
GCACAAACCAGGGAAAGCCCCTAAGCTCCTGATCTACGATACATCCAATT17280.22077085824671144No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCT16660.21284968161980397No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGCTCTCCAGCTTGGTCC16550.21144431157309457No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGAGCAAACGAACTGTGGCTGCACCA15940.20365089585952434No Hit
AGTCAGGACATTAACTACTATTTAAATTGGTATCAGCACAAACCAGGGAA15620.19956254663273335No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15170.1938133055325586No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT15100.1929189791391981No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT14740.18831958625905826No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC14730.18819182534572104No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG14000.17886527867210417No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA13810.17643782131869706No Hit
GTCTCCATCTTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT13800.17631006040535982No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13520.17273275483191775No Hit
CTCCAGCTTGGTCCCCTGGCCAAAAGTGTAAGGGAGATTATCATACTGTT13410.17132738478520834No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13200.16864440560512678No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12740.16276740359161482No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT12710.16238412085160314No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12650.16161755537157985No Hit
GGCCAAAAGTGTAAGGGAGATTATCATACTGTTGACAGAAATATGTTGCA12640.16148979445824263No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG12640.16148979445824263No Hit
GATTTTACTTTAACCATCAGCAGCCTGCAGCCTGAAGATATTGCAACATA12490.15957338075818436No Hit
AAGCTGGAGAGCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCC12250.15650711883809115No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12220.15612383609807948No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12080.15433518331135845No Hit
ATCTACGATACATCCAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTGG12060.15407966148468402No Hit
CCATCTTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCA11920.15229100869796297No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11900.15203548687128854No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC11760.15024683408456752No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11750.15011907317123027No Hit
GCCTGAAGATATTGCAACATATTTCTGTCAACAGTATGATAATCTCCCTT11710.14960802951788144No Hit
CATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTACTTTAACCATC11680.14922474677786976No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC11660.14896922495119533No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG11580.1479471376444976No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11410.1457752021177649No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT11400.14564744120442769No Hit
GGTTTGTGCTGATACCAATTTAAATAGTAGTTAATGTCCTGACTCGCCTG11350.1450086366377416No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCC11100.14181461380431115No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11090.14168685289097396No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11040.14104804832428786No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA10950.1398982001042529No Hit
ACCTTGATGGGACCCCTGTTTCCAAATTGGATGTATCGTAGATCAGGAGC10860.13874835188421794No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10740.13721522092417132No Hit
GGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCT10740.13721522092417132No Hit
TCCCTGGTTTGTGCTGATACCAATTTAAATAGTAGTTAATGTCCTGACTC10740.13721522092417132No Hit
ATCTTCAGGCTGCAGGCTGCTGATGGTTAAAGTAAAATCTGTCCCAGATC10680.13644865544414803No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10660.13619313361747362No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10610.13555432905078751No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10570.13504328539743865No Hit
GTTCAGTGGAAGTGGATCTGGGACAGATTTTACTTTAACCATCAGCAGCC10560.13491552448410143No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC10480.13389343717740368No Hit
ATCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTGTGCTGATACCAAT10470.1337656762640665No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10220.13057165343063604No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT10140.1295495661239383No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGA10060.12852747881724058No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10060.12852747881724058No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10060.12852747881724058No Hit
CCCCTAAGCTCCTGATCTACGATACATCCAATTTGGAAACAGGGGTCCCA10020.12801643516389172No Hit
GTGGAAGTGGATCTGGGACAGATTTTACTTTAACCATCAGCAGCCTGCAG9940.12699434785719396No Hit
GATCTACGATACATCCAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTG9890.12635554329050788No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9860.1259722605504962No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA9570.12226719406371693No Hit
CAGTATGATAATCTCCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGAG9450.12073406310367032No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC9440.1206063021903331No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9240.11805108392358875No Hit
ACATATTTCTGTCAACAGTATGATAATCTCCCTTACACTTTTGGCCAGGG9160.11702899661689102No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9110.11639019205020493No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGGGTCAGGGACA8880.11345169104344892No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT8770.11204632099673954No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8770.11204632099673954No Hit
ATCATACTGTTGACAGAAATATGTTGCAATATCTTCAGGCTGCAGGCTGC8640.11038542912335572No Hit
GAAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTTAAAGTAA8460.1080857326832858No Hit
GGACATTAACTACTATTTAAATTGGTATCAGCACAAACCAGGGAAAGCCC8420.10757468902993694No Hit
TATTCATTCTCCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGG8410.10744692811659971No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCT8340.1065526017232392No Hit
GTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAG8310.10616931898322754No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT8300.10604155806989034No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAAC8270.10565827532987868No Hit
GCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCAT8220.10501947076319258No Hit
GACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTC8220.10501947076319258No Hit
GTCTCCTACAGATGCAGACAGGGAAGATGGAGACTGGGTCATCTGGATGT8180.10450842710984373No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG7990.1020809697564366No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA7860.10042007788305277No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)