FastQCFastQC Report
Wed 8 May 2024
EGAF00004717718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences782712
Total Bases57.6 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT44130.5638089105571398No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36120.46147241897402874No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30730.3926092866852687No Hit
GTATGATAATCTCCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGAGCA28450.3634797984443831No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28030.35811384008422No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG27640.3531311644640685No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT25120.32093541430308975No Hit
TTATCATACTGTTGACAGAAATATGTTGCAATATCTTCAGGCTGCAGGCT24240.3096924539294147No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24040.3071372356626703No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23570.3011324727358211No Hit
CCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGAGCAAACGAACTGTGG22710.2901450341888204No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22420.2864399677020411No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22140.28286266212859906No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22010.2812017702552152No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA20760.26523165608806304No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAACTA20700.26446509060803974No Hit
GTGTAAGGGAGATTATCATACTGTTGACAGAAATATGTTGCAATATCTTC20310.25948241498788827No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20030.25590510941444616No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19810.25309436932102736No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19810.25309436932102736No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19130.24440662721409664No Hit
AACCAGGGAAAGCCCCTAAGCTCCTGATCTACGATACATCCAATTTGGAA19110.24415110538742218No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC18880.2412126043806662No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC18720.2391684297672707No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC18700.2389129079405963No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG18650.2382741033739102No Hit
GTGCAGCCACAGTTCGTTTGCTCTCCAGCTTGGTCCCCTGGCCAAAAGTG18280.23354694958043318No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA18190.23239710136039818No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG18150.23188605770704931No Hit
GCACAAACCAGGGAAAGCCCCTAAGCTCCTGATCTACGATACATCCAATT16560.2115720724864318No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC16290.2081225278263269No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG15620.19956254663273335No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGCTCTCCAGCTTGGTCC15370.19636852379930295No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCT15130.1933022618792097No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15050.19228017457251198No Hit
AGTCAGGACATTAACTACTATTTAAATTGGTATCAGCACAAACCAGGGAA14820.18934167356575599No Hit
CTCCAGCTTGGTCCCCTGGCCAAAAGTGTAAGGGAGATTATCATACTGTT14640.18704197712568607No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT13900.177587669538732No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGAGCAAACGAACTGTGGCTGCACCA13820.17656558223203425No Hit
GTCTCCATCTTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT13640.17426588579196436No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA13500.1724772330052433No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA13360.1706885802185223No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12940.16532262185835914No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT12830.16391725181164976No Hit
GGCCAAAAGTGTAAGGGAGATTATCATACTGTTGACAGAAATATGTTGCA12130.15497398787804453No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG12120.15484622696470735No Hit
GATTTTACTTTAACCATCAGCAGCCTGCAGCCTGAAGATATTGCAACATA12020.15356861783133516No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT11960.15280205235131183No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11930.15241876961130021No Hit
ATCTACGATACATCCAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTGG11520.1471805721644743No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA11520.1471805721644743No Hit
ACCTTGATGGGACCCCTGTTTCCAAATTGGATGTATCGTAGATCAGGAGC11520.1471805721644743No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG11500.14692505033779987No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11430.14603072394443933No Hit
AAGCTGGAGAGCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCC11350.1450086366377416No Hit
GGTTTGTGCTGATACCAATTTAAATAGTAGTTAATGTCCTGACTCGCCTG11350.1450086366377416No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11300.1443698320710555No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC11230.143475505677695No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC11190.14296446202434612No Hit
CATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTACTTTAACCATC11160.14258117928433447No Hit
ATCTTCAGGCTGCAGGCTGCTGATGGTTAAAGTAAAATCTGTCCCAGATC11090.14168685289097396No Hit
CCATCTTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCA10940.1397704391909157No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10920.13951491736424126No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10830.1383650691442063No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCC10750.13734298183750857No Hit
GCCTGAAGATATTGCAACATATTTCTGTCAACAGTATGATAATCTCCCTT10740.13721522092417132No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10700.13670417727082246No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGA10640.1359376117907992No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10580.13517104631077587No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC10500.13414895900407814No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10360.1323603062173571No Hit
TCCCTGGTTTGTGCTGATACCAATTTAAATAGTAGTTAATGTCCTGACTC10300.1315937407373338No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10170.12993284886394996No Hit
GGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCT10140.1295495661239383No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9920.12673882603051953No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT9920.12673882603051953No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9710.12405584685043797No Hit
ATCGTAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTGTGCTGATACCAAT9610.1227782377170658No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9600.12265047680372856No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC9600.12265047680372856No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9550.12201167223704248No Hit
GTGGAAGTGGATCTGGGACAGATTTTACTTTAACCATCAGCAGCCTGCAG9470.12098958493034476No Hit
GTTCAGTGGAAGTGGATCTGGGACAGATTTTACTTTAACCATCAGCAGCC9350.11945645397029815No Hit
CCCCTAAGCTCCTGATCTACGATACATCCAATTTGGAAACAGGGGTCCCA9280.11856212757693763No Hit
ATCATACTGTTGACAGAAATATGTTGCAATATCTTCAGGCTGCAGGCTGC8970.11460153926348389No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8940.11421825652347224No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8850.11306840830343728No Hit
GATCTACGATACATCCAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTG8710.11127975551671625No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCA8690.11102423369004179No Hit
ACATATTTCTGTCAACAGTATGATAATCTCCCTTACACTTTTGGCCAGGG8620.11012990729668128No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8580.10961886364333241No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT8530.10898005907664632No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGGGTCAGGGACA8490.10846901542329745No Hit
GTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAG8480.10834125450996024No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8450.1079579717699486No Hit
CAGTATGATAATCTCCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGAG8430.10770244994327415No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8410.10744692811659971No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA8260.10553051441654145No Hit
GAAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTTAAAGTAA8170.1043806661965065No Hit
GACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTC8110.1036141007164832No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT8080.10323081797647155No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT8010.10233649158311103No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7910.10105888244973885No Hit
GGACATTAACTACTATTTAAATTGGTATCAGCACAAACCAGGGAAAGCCC7840.10016455605637833No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAAC7840.10016455605637833No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)