FastQCFastQC Report
Wed 8 May 2024
EGAF00004717721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1084371
Total Bases80 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT67470.6222040242684469No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC58920.5433564711708446No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC57740.5324745866497721No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44860.4136960505214544No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG41870.38612246177738063No Hit
GTCTGGGACAGAGTTCACTCTTACCATCAGCAGCCTGCAGTCTGAAGATT41590.3835403196876346No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC38720.3570733632677377No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT38540.3554134147814724No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG36750.3389061492791674No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGCATCCCAGGCAGGTTCAGTG35560.32793204539774673No Hit
GTACTGGAGAAATAGTGATGACGCAGTCTCCAGTCACCCTGTCTGTGTCT35470.32710207115461404No Hit
GTCCCAGACCCACTGCCACTGAACCTGCCTGGGATGCCAGTGGCCCTGGT35050.323228858019995No Hit
CTCCTGCAGGACCAGTCAGAGTGTTTACAGCAACTTAGCCTGGTACCAGC33500.30893485716604374No Hit
GAGTTCACTCTTACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTA33350.3075515667608226No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32670.30128065025715367No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32660.30118843089680564No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32640.30100399217610946No Hit
TTACTATAGAGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCT31100.2868022106825063No Hit
GTCTGTGTCTCCAGGGGAAACAGCCACCCTCTCCTGCAGGACCAGTCAGA31040.2862488945204178No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30450.28080795225988153No Hit
GTCTCCAGTCACCCTGTCTGTGTCTCCAGGGGAAACAGCCACCCTCTCCT29550.272508209828555No Hit
CTATAGAGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCT29460.2716782355854223No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC29140.268727216054284No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTTCTCTGGCTC28990.2673439256490629No Hit
CTATAGTAATTGGCCCGCGCTCACTTTCGGCGGAGGGACCACGGTGGAGA28960.2670672675680187No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA28880.26632951268523414No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC28500.2628251769920073No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA28360.26153410594713433No Hit
GCTCTATAGTAATTGGCCCGCGCTCACTTTCGGCGGAGGGACCACGGTGG28220.2602430349022613No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCGTGGTCCCTCCGCCGAAAGTG28060.25876752513669216No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27430.2529577054347636No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26600.2453034985258735No Hit
GTTTATTACTGTCAGCTCTATAGTAATTGGCCCGCGCTCACTTTCGGCGG25840.23829482713942No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT25380.23405273656340866No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGCCTGGGATGCCA24460.22556855541138598No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTAAGAGTGA24060.22187978099746306No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC24010.2214186841957227No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCGTGGTCC23130.2133033804850923No Hit
AGATAGTACTGGAGAAATAGTGATGACGCAGTCTCCAGTCACCCTGTCTG22510.20758578014351178No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG21940.20232927660367162No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT21860.20159152172088704No Hit
CTGCAGGACCAGTCAGAGTGTTTACAGCAACTTAGCCTGGTACCAGCAGA21670.19983935387427365No Hit
CTCTTACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGT21560.19882494091044486No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGTCACCCTGTCTGTGTCTCCAGG20960.1932917792895605No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC20770.19153961144294712No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA20250.18674420470484732No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19980.18425428197544935No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19870.18323986901162057No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19300.1779833654717804No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19300.1779833654717804No Hit
ATAGTAATTGGCCCGCGCTCACTTTCGGCGGAGGGACCACGGTGGAGATC19280.17779892675108427No Hit
CACCAGGGCCACTGGCATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGA19110.17623119762516704No Hit
TCTATGGTGCATCCACCAGGGCCACTGGCATCCCAGGCAGGTTCAGTGGC19010.17530900402168628No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18870.17401793297681328No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18750.1729113006526364No Hit
GTCAGAGTGTTTACAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAG18570.1712513521663711No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC18360.16931474559906157No Hit
TGGTAAGAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGCCTGGG18080.16673260350931554No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17920.16525709374374636No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTTCTCT17700.1632282678160888No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG17510.16147609996947537No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17180.15843286107798898No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC16620.15326857689849693No Hit
CCCTGTCTGTGTCTCCAGGGGAAACAGCCACCCTCTCCTGCAGGACCAGT16610.15317635753814884No Hit
GTTTACAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAG16370.1509630928897951No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16250.14985646056561824No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCC15930.1469054410344799No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCA15730.14506105382751844No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15640.14423107958438577No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTGCTGTAA15570.14358554406194926No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT15560.1434933247016012No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15310.14118784069289939No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15270.14081896325150708No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14930.13768350499967263No Hit
CTCCCAGATAGTACTGGAGAAATAGTGATGACGCAGTCTCCAGTCACCCT14900.1374068469186284No Hit
CTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGCATCCCA14900.1374068469186284No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC14590.13454804674783816No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA14510.13381029186505358No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC14440.13316475634261707No Hit
CCTCATCTATGGTGCATCCACCAGGGCCACTGGCATCCCAGGCAGGTTCA14360.13242700145983247No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14280.1316892465770479No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14100.1300292980907826No Hit
GCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTT14050.12956820128904223No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTTCTCTGGCTCCCAGATAGTACT13930.12846156896486535No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13920.12836934960451726No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13910.1282771302441692No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13850.12772381408208078No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13710.12643274303720775No Hit
GTCCTGCAGGAGAGGGTGGCTGTTTCCCCTGGAGACACAGACAGGGTGAC13640.12578720751477124No Hit
CCAGTCACCCTGTCTGTGTCTCCAGGGGAAACAGCCACCCTCTCCTGCAG13630.12569498815442315No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG13580.12523389135268279No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT13560.12504945263198666No Hit
GCTTCTCTTCCTCCTGCTTCTCTGGCTCCCAGATAGTACTGGAGAAATAG13440.12394282030780979No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTTACCATCAGCAGCC13320.12283618798363291No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT13210.1218217750198041No Hit
GGTTTCTGCTGGTACCAGGCTAAGTTGCTGTAAACACTCTGACTGGTCCT13110.12089958141632338No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12980.11970072973179843No Hit
GAGTGTTTACAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTC12970.11960851037145034No Hit
AATTACTATAGAGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGG12960.11951629101110228No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12940.11933185229040615No Hit
GAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCT12920.11914741356971No Hit
CAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTAAGAGT12610.11628861339891974No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12550.11573529723683129No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA12510.115366419795439No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12480.1150897617143948No Hit
ATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTTCTCTGGCTCCCAGA12340.11379869066952178No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC12250.11296871642638912No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA12120.11176986474186418No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCT12040.11103210985907959No Hit
TTATTACTGTCAGCTCTATAGTAATTGGCCCGCGCTCACTTTCGGCGGAG11790.10872662585037778No Hit
GGACCAGTCAGAGTGTTTACAGCAACTTAGCCTGGTACCAGCAGAAACCT11600.10697445800376439No Hit
CTCCAGTCACCCTGTCTGTGTCTCCAGGGGAAACAGCCACCCTCTCCTGC11480.10586782567958751No Hit
CTGCAGGAGAGGGTGGCTGTTTCCCCTGGAGACACAGACAGGGTGACTGG11460.10568338695889137No Hit
GTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCC11350.10466897399506257No Hit
GGCCAATTACTATAGAGCTGACAGTAATAAACTGCAAAATCTTCAGACTG11240.10365456103123379No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG10990.10134907702253196No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10980.10125685766218387No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG10910.10061132213974738No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)