FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1084371
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT62200.5736044213650126No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT48230.4447739749587549No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTTCTCTGGCTCCCAGATAGT46570.4294655611409748No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37090.3420416075310019No Hit
GTCTGGGACAGAGTTCACTCTTACCATCAGCAGCCTGCAGTCTGAAGATT35950.33152860045132154No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA35230.3248888065062603No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33120.30543052147281696No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32860.3030328181037671No Hit
GTCCCAGACCCACTGCCACTGAACCTGCCTGGGATGCCAGTGGCCCTGGT32440.299159604969148No Hit
GTACTGGAGAAATAGTGATGACGCAGTCTCCAGTCACCCTGTCTGTGTCTCCAGGGGAAACAGCCACCCTCTC31020.2860644557997217No Hit
GAGTTCACTCTTACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCTCTATAGTAATTGG30320.2796091005753566No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT29950.27619698424247785No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG29860.27536700999934527No Hit
GTCTGTGTCTCCAGGGGAAACAGCCACCCTCTCCTGCAGGACCAGTCAGAGTGTTTACAGCAACTTAGCCTGGT28480.2626407382713112No Hit
TTACTATAGAGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTAAGAGTGAACTCTGTC28320.26116522850574203No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGCATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTC28280.26079635106434973No Hit
CTATAGAGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTAAGAGTGAACTCTGTCCC28060.25876752513669216No Hit
CTCCTGCAGGACCAGTCAGAGTGTTTACAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT26780.24696344701213885No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG25960.23940145946359687No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA25930.23912480138255265No Hit
CTATAGTAATTGGCCCGCGCTCACTTTCGGCGGAGGGACCACGGTGGAGATCAAACGAACTGTGGCTGCACCAT25760.2375570722566354No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC25230.23266944615818755No Hit
GTCTCCAGTCACCCTGTCTGTGTCTCCAGGGGAAACAGCCACCCTCTCCTGCAGGACCAGTCAGAGTGTTTAC24780.22851957494252426No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24540.22630631029417056No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC24290.22400082628546872No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23890.2203120518715458No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23730.21883654210597664No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTTCTCTGGCTC23710.2186521033852805No Hit
GCTCTATAGTAATTGGCCCGCGCTCACTTTCGGCGGAGGGACCACGGTGGAGATCAAACGAACTGTGGCTGCACC23410.21588552257483828No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTAAGAGTGAACTCTGTCCCAGACCCACTGCC23380.21560886449379413No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCGTGGTCCCTCCGCCGAAAGTGAGCGCGGGCCAATTACTATAG23340.2152399870524018No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGCCTGGGATGCCAGTGGCCCTGGTGGATGCACCAT23170.2136722579264846No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG23050.21256562560230768No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC22430.2068480252607272No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC22030.20315925084680428No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21320.19661167626209114No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTTCTCTGGCTCCC21250.1959661407396546No Hit
GTTTATTACTGTCAGCTCTATAGTAATTGGCCCGCGCTCACTTTCGGCGGAGGGACCACGGTGGAGATCAAACGA21170.19522838585687002No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA20810.1919084888843394No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20690.19080185656016252No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC20130.18563757238067047No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT19150.17660007506655934No Hit
CTGCAGGACCAGTCAGAGTGTTTACAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCT18890.17420237169750943No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18760.17300352001298447No Hit
CTCTTACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCTCTATAGTAATTGGCCCGCG18630.17180466832845953No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGTCACCCTGTCTGTGTCTCCAGGGGAAACAGCCACCCTCTCCTGCAGG18070.16664038414896748No Hit
AGATAGTACTGGAGAAATAGTGATGACGCAGTCTCCAGTCACCCTGTCTGTGTCTCCAGGGGAAACAGCCACCCT17760.16378158397817721No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC17290.1594472740418178No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17030.1570495706727679No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCGTGGTCCCTCCGCCGAAAGTGAGCGCGGGCCA16990.15668069323137562No Hit
ATAGTAATTGGCCCGCGCTCACTTTCGGCGGAGGGACCACGGTGGAGATCAAACGAACTGTGGCTGCACCAT16820.15511296410545838No Hit
CACCAGGGCCACTGGCATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTTACCATCAGC16740.1543752092226738No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT16700.1540063317812815No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16420.15142418969153545No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16210.14948758312422594No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC16140.14884204760178943No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC15930.1469054410344799No Hit
GTCAGAGTGTTTACAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTG15560.1434933247016012No Hit
TCTATGGTGCATCCACCAGGGCCACTGGCATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT15560.1434933247016012No Hit
TGGTAAGAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGCCTGGGATGCCAGTGGCCCTGGTGGATGCAC15530.143216666620557No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15470.14266335045846856No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTTCTCTGGCTCCCAGATAGTACTGGAGAAAT14990.13823682116176106No Hit
CCCTGTCTGTGTCTCCAGGGGAAACAGCCACCCTCTCCTGCAGGACCAGTCAGAGTGTTTACAGCAACTTAGC14430.13307253698226898No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14420.13298031762192092No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT14320.1320581240184402No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG14040.12947598192869414No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13430.12385060094746171No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC13290.12255952990258868No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13150.12126845885771567No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13150.12126845885771567No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC13040.12025404589388687No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12980.11970072973179843No Hit
GTTTACAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACC12930.11923963293005808No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12780.11785634252483698No Hit
CTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGCATCCCA12780.11785634252483698No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGCATCCCAGGCAGGTT12750.11757968444379276No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTTCTCTGGCTCCCAGATAGTACTGGAGAAATAGTGATGACGCAGTCTC12710.11721080700240047No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTTACCATCAGCAGCC12690.11702636828170433No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12510.115366419795439No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12510.115366419795439No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCC12450.11481310363335058No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12440.11472088427300252No Hit
CCTCATCTATGGTGCATCCACCAGGGCCACTGGCATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTT12300.11342981322812948No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA12220.1126920583453449No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT12150.1120465228229084No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTGCTGTAAACACTCTGACTGGTCCTGCAGGAG12110.11167764538151612No Hit
GAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTTCTCTGGCTC12080.11140098730047189No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12060.11121654857977573No Hit
AATTACTATAGAGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTAAGAGTGAACT11960.11029435497629501No Hit
GCTTCTCTTCCTCCTGCTTCTCTGGCTCCCAGATAGTACTGGAGAAATAGTGATGACGCAGTCTCCAGTCACC11960.11029435497629501No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCC11950.11020213561594694No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11630.10725111608480861No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC11390.10503785143645486No Hit
GCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTG11170.10300902550879727No Hit
ATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTTCTCTGGCTCCCAGATAGTACTGGAGAAATAGTGATGACG11000.10144129638288003No Hit
GGTTTCTGCTGGTACCAGGCTAAGTTGCTGTAAACACTCTGACTGGTCCTGCAGGAGAGGGTGGCTGTTTCCC10920.10070354150009544No Hit
GAGTGTTTACAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATC10890.10042688341905122No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)