FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992262
Sequences flagged as poor quality0
Sequence length18-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT56370.5680959262775356No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54060.5448157845407765No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA40410.4072513106417458No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT39830.4014060802489665No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA33980.3424498771493819No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT32150.32400716746181957No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31510.31755725806289065No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT29560.2979051903630291No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28050.2826874353749312No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC27330.2754312873011362No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26890.27099697458937255No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAACTG24940.251344906889511No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC24880.2507402278833614No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA24550.24741449334953874No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCA24250.2443910983187908No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTACCAGCAGAAAGCTGGGCAGGCTCCCAGGCT24060.2424762814659838No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23530.23713495024499578No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGTGGATGCACCATAGATGACGAGCCTG23460.2364294914044879No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCT22780.22957646266812595No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC21730.21899458006050823No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTA20140.2029705863975442No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA19380.19531131898631612No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19280.19430352064273348No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19240.19390040130530042No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT18690.18835751041559587No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA18610.18755127174072975No Hit
GTTTATTACTGTCAGCAGTATAATAACTGGCCTCCGATCACCTTCGGCCA18510.18654347339714714No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCACTGGAGAAATAGT18400.18543489521920622No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGGAGGCCAGTTATTATACT18300.18442709687562356No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18270.18412475737254877No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18270.18412475737254877No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA17070.17203117724955708No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCA16820.16951168139060047No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16640.1676976443721517No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGGAGGCCA15780.159030578617341No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGTGGATGCACCATA15120.15237910954969555No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAG15040.15157287087482943No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14770.1488518153471563No Hit
CCACTGGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTC14760.14875103551279803No Hit
GTCAGAGTGTTAGCAGCAACTTAGCCTGGTACCAGCAGAAAGCTGGGCAGGCTCCCAGGCTCGTCATCTATGGT14740.1485494758440815No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC14640.14754167750049885No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGG14610.14723933799742406No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA14540.14653387915691624No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14500.14613075981948315No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14160.14270424545130217No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT14080.14189800677643605No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13640.13746369406467243No Hit
CTGCTACTCTGGCTCCCAGACACCACTGGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCA13590.13695979489288113No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13560.1366574553898063No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGGAGGCCAGTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCA13190.13292860151855054No Hit
CTCCCAGGCTCGTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTG13110.13212236284368442No Hit
GGATACCAGTGGCCCTGGTGGATGCACCATAGATGACGAGCCTGGGAGCCTGCCCAGCTTTCTGCTGGTACCAG12910.13010676615651914No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12840.1294013073160113No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12610.1270833711257712No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG12530.1262771324509051No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCT12050.12143970040170843No Hit
GGCCGAAGGTGATCGGAGGCCAGTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCT12030.1212381407329919No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCA11880.11972644321761794No Hit
GTGTTAGCAGCAACTTAGCCTGGTACCAGCAGAAAGCTGGGCAGGCTCCCAGGCTCGTCATCTATGGTGCATCCA11760.11851708520531874No Hit
GAGTGTTAGCAGCAACTTAGCCTGGTACCAGCAGAAAGCTGGGCAGGCTCCCAGGCTCGTCATCTATGGTGCATC11730.11821474570224395No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11720.1181139658678857No Hit
TTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC11600.11690460785558654No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11540.11629992884943695No Hit
TCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT11500.11589680951200389No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11480.11569524984328734No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11470.11559447000892908No Hit
CACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCA11400.11488901116842125No Hit
TTATTACTGTCAGCAGTATAATAACTGGCCTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA11310.11398199265919685No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA11290.11378043299048034No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTT11150.11236951530946464No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC10950.11035391862229936No Hit
GCAGAAAGCTGGGCAGGCTCCCAGGCTCGTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTT10640.10722974375719316No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTG10600.1068266244197601No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10590.10672584458540184No Hit
CCATAGATGACGAGCCTGGGAGCCTGCCCAGCTTTCTGCTGGTACCAGGCTAAGTTGCTGCTAACACTCTGACTG10580.10662506475104358No Hit
CAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA10580.10662506475104358No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAGCC10420.10501258740131135No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10410.10491180756695309No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGG10390.10471024789823656No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA10220.10299699071414606No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCACTGGAGAAATAGTGATGACGCAGTCTC10120.1019891923705634No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGAT10070.1014852931987721No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)