FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1261431
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51510.4083457596967254No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT40850.323838561126213No Hit
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACAGTGTGTTCTTGGAATTGTCTCTGG40480.3209053844403697No Hit
GGGTATTACCAGCTGGTTAGCCTGGTATCAGCAGAGACCAGGGAAAGCCCCTAAGTTACTGATCTATGGTGCATC39750.31511830611424646No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35310.2799201858841268No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCC32430.2570889727618871No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32260.25574129698731046No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA31360.24860654288661055No Hit
GATCTATGGTGCATCCAGTTTGCAAAGCGGGGTCCCATCAAGGTTCAGCGGCAGTGGGTCTGGGACAGATTTC29710.23552616036866067No Hit
GGCTAACAGTTTCCCTATCACTTTCGGCCCTGGGACCAAAGTGGATGTCAAACGAACTGTGGCTGCACCAT29350.23267225872838068No Hit
CTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATAGTGAGAGTGAAATCTGTC27900.2211773771216975No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27750.21998825143824752No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27010.21412189806656093No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT26890.21317059751980094No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG25600.20294411664213102No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC22970.1820947796589746No Hit
CACTATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTAACAGTTTCCCTATCACTTT22400.17757610206186467No Hit
GTGCAGCCACAGTTCGTTTGACATCCACTTTGGTCCCAGGGCCGAAAGTGATAGGGAAACTGTTAGCCTGTTGAC22390.17749682701630132No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21730.17226467400912138No Hit
CTCTAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTACATAGCATAGCTTCTGAAGGTGAATCCAGAGGCTGCAC21640.17155119859905138No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG21400.16964859750553143No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21070.16703252100194144No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20940.16600194540961813No Hit
CCATAGATCAGTAACTTAGGGGCTTTCCCTGGTCTCTGCTGATACCAGGC20800.16489209477173147No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC20060.15902574140004486No Hit
GTCCCAGACCCACTGCCGCTGAACCTTGATGGGACCCCGCTTTGCAAACTGGATGCACCATAGATCAGTAACTT19790.1568853151698349No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG19760.1566474900331449No Hit
GTCTGGGACAGATTTCACTCTCACTATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGG19660.1558547395775116No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC19420.15395213848399159No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19280.15284228784610493No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC19100.15141533702596496No Hit
GTGTATTACTGTGCGAGAGACCGAGTGGTGGTAGCTTCCAACTGGATCGACCCCTGGGGCCAGGGAACCCTGGTC18510.14673810933772832No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT18030.14293290715068838No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17880.1417437814672384No Hit
GGGCTAGAGTGGGTGGCATTTATATCATATGATGGAAGGAATAAAAACTACGCAGACTCCGTGAAGGGCCGATTC17820.1412681311938584No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACATCCACTTTGGTCCCAGGGCCGAAAGTGATAGGGAA17460.13841422955357843No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17200.1363530783689318No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT16800.1331820765463985No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA16780.13302352645527182No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16650.13199295086294852No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC16350.12961469949604854No Hit
GACCAGGGAAAGCCCCTAAGTTACTGATCTATGGTGCATCCAGTTTGCAA15970.1266022477646419No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCC15270.12105299457520864No Hit
GTAATACCCTGACTCGCCCGACAAGTGATGGTGACTGTGTCTCCTACAGA15270.12105299457520864No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15050.11930894357281532No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14770.11708924229704201No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC14730.11677214211478867No Hit
GCTGATAGTGAGAGTGAAATCTGTCCCAGACCCACTGCCGCTGAACCTTG14720.11669286706922535No Hit
GTAGGAGACACAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTACCAGCTGGTTAGCCTGGTATCAGCAG14720.11669286706922535No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14520.11510736615795869No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14240.11288766488218539No Hit
CAGTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATA14190.11249128965436872No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC13930.11043013846972209No Hit
GGTAATACCCTGACTCGCCCGACAAGTGATGGTGACTGTGTCTCCTACAG13680.10844826233063877No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13600.10781406196613212No Hit
GCTATGTACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTAGAGTGGGTGGCATTTATATCATATGATGGAAGGAAT13560.10749696178387878No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC13530.1072591366471888No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT13420.10638711114599213No Hit
GATTTCACTCTCACTATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTAACAGTTTC13340.10575291078148547No Hit
TCCCTGGTCTCTGCTGATACCAGGCTAACCAGCTGGTAATACCCTGACTCGCCCGACAAGTGATGGTGACTGTGT13300.10543581059923215No Hit
ATTGTCAACAGGCTAACAGTTTCCCTATCACTTTCGGCCCTGGGACCAAAGTGGATGTCAAACGAACTGTGGCTG13260.10511871041697882No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13260.10511871041697882No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC13160.1043259599613455No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13010.1031368342778955No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12940.10258190895895217No Hit
AGTCAGGGTATTACCAGCTGGTTAGCCTGGTATCAGCAGAGACCAGGGAA12670.1004414827287422No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)