FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1392630
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCAATTTCCTACCA72830.5229673351859431No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG67730.4863459784723868No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC67250.48289926254640503No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCAATTTCCTACCAGTTACATATGAGCA64130.46049560902752346No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAGGCAACAGTGATGTTGGGAGTTATAACTTCATCTCCTGGT57670.414108557190352No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT55210.3964441380696955No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA50120.3598945879379304No Hit
CTCCTGCACTGGAGGCAACAGTGATGTTGGGAGTTATAACTTCATCTCCTGGTACCTACATCACCCCGGCCAAGC47130.3384244199823356No Hit
TTATTATTGTTGCTCATATGTAACTGGTAGGAAATTGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTC45630.32765343271364256No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC45400.32600188133244296No Hit
GTACTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT45400.32600188133244296No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG45220.32470936286019975No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC44170.31716967177211464No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC42430.3046753265404307No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTACTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT39890.2864364547654438No Hit
GTCTGGAGCCCAGAAATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC37190.2670486776817963No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG36930.26518170655522283No Hit
GTCGATCACCATCTCCTGCACTGGAGGCAACAGTGATGTTGGGAGTTATAACTTCATCTCCTGGTACCTACATC35010.25139484285129576No Hit
GTCTTCTCCACGGTACTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC34030.24435779783574962No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC33910.24349611885425415No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG33480.24040843583722885No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCAATTTCCTACCAGTTACATATGAGCAACAATAATAATG33180.23825423838349022No Hit
ATTCAGGGGTTTCTAACCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATTTCTGGGCTCC33180.23825423838349022No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC32890.23617184751154294No Hit
ACATCACCCCGGCCAAGCCCCCAAAGCCCTGATTTATGAGGTCAATCAACGGTATTCAGGGGTTTCTAACCGCTT32820.2356692014390039No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG32250.23157622627690053No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC31950.22942202882316193No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC31770.22812951035091877No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC31570.22669337871509304No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG31340.2250418273338934No Hit
CAATAATAATGACCCTCGTCCTCAGTCTGGAGCCCAGAAATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC31160.22374930886165026No Hit
GTATTCAGGGGTTTCTAACCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATTTCTGGGCTCC30770.22094885217179006No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT30340.21786116915476472No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG30240.21714310333685186No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA29820.21412722690161778No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA29700.21326554792012237No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC29210.20974702541234927No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT28550.20500779101412436No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC28210.2025663672332206No Hit
GCTCCAGACTGAGGACGAGGGTCATTATTATTGTTGCTCATATGTAACTG27630.19840158548932596No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC26980.19373415767289232No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTG26400.18956937592899764No Hit
CCTTGGTCCCTCCGCCGAACACCAATTTCCTACCAGTTACATATGAGCAA25930.18619446658480718No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA25240.18123981244120835No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGG24920.17894200182388717No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG24830.1782957425877656No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC24730.17757767676985273No Hit
GATTTATGAGGTCAATCAACGGTATTCAGGGGTTTCTAACCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTC24380.17506444640715768No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACC24210.1738437345167058No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA24200.17377192793491453No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC22910.1645088788838385No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA22900.1644370723020472No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC22730.16321636041159532No Hit
CCCCTGAATACCGTTGATTGACCTCATAAATCAGGGCTTTGGGGGCTTGGCCGGGGTGATGTAGGTACCAGG21810.15661015488679692No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC21510.15445595743305832No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG21280.15280440605185872No Hit
GTTACATATGAGCAACAATAATAATGACCCTCGTCCTCAGTCTGGAGCCCAGAAATTGTCAGGGAGGCCGTGTTG21100.15151188757961553No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC20810.14942949670766822No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT20780.14921407696229436No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACA20620.14806517165363378No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTACTCCCTTCATGCGTGACCTGGC20360.1461982005270603No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCCCAGGACCCTGTGTCCTGAGTGAGG20320.14591097419989515No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAGGCA20180.14490568205481716No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC19870.14267967801928724No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC19760.1418898056195831No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACAC19260.13829947653001873No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCAATTTCCTAC18940.13600166591269755No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC18920.13585805274911497No Hit
GGGTGATGTAGGTACCAGGAGATGAAGTTATAACTCCCAACATCACTGTT18830.13521179351299342No Hit
TATGTAACTGGTAGGAAATTGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC18450.1324831434049245No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGG18420.13226772365955064No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT18340.13169327100522035No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGG18310.13147785125984648No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC18190.13061617227835104No Hit
GTTAGAAACCCCTGAATACCGTTGATTGACCTCATAAATCAGGGCTTTGGGGGCTTGGCCGGGGTGATGTAGGT18080.12982629987864688No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA18040.12953907355148173No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17900.12853378140640373No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC17830.1280311353338647No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGC17790.12774390900669955No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTACTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCC17670.1268822300252041No Hit
ACGGTATTCAGGGGTTTCTAACCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATTTCTGGGCT17430.1251588720622132No Hit
ACCTACATCACCCCGGCCAAGCCCCCAAAGCCCTGATTTATGAGGTCAAT16890.12128131664548372No Hit
GTATCAACGCAGAGTACGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTG16710.11998879817324057No Hit
CCTGAATACCGTTGATTGACCTCATAAATCAGGGCTTTGGGGGCTTGGCCGGGGTGATGTAGGTACCAGGAGATG16630.11941434551891027No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC16520.1186244731192061No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA16340.11733195464696294No Hit
GAATACCGTTGATTGACCTCATAAATCAGGGCTTTGGGGGCTTGGCCGGG16310.11711653490158909No Hit
GCCCTGATTTATGAGGTCAATCAACGGTATTCAGGGGTTTCTAACCGCTT16300.11704472831979779No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCAC16140.11589582301113721No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA16130.11582401642934591No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTACTCCCTTCATGCGTGACC16040.11517775719322434No Hit
TCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCC15940.11445969137531145No Hit
GTACCTACATCACCCCGGCCAAGCCCCCAAAGCCCTGATTTATGAGGTCAATCAACGGTATTCAGGGGTTTCTA15690.11266452683052929No Hit
GGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT15690.11266452683052929No Hit
ACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG15660.11244910708515543No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT15480.11115658861291226No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT15160.10885877799559109No Hit
CTACATCACCCCGGCCAAGCCCCCAAAGCCCTGATTTATGAGGTCAATCAACGGTATTCAGGGGTTTCTAACCG15120.10857155166842593No Hit
TCCTCAGTCTGGAGCCCAGAAATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGGTTAGAAACC15000.10770987268693048No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC14900.10699180686901762No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAG14750.10591470814214832No Hit
AATCAGGGCTTTGGGGGCTTGGCCGGGGTGATGTAGGTACCAGGAGATGA14710.10562748181498316No Hit
GATGTAGGTACCAGGAGATGAAGTTATAACTCCCAACATCACTGTTGCCTCCAGTGCAGGAGATGGTGATCG14500.10411954359736614No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGC14480.10397593043378356No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCC14350.10304244487049682No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG14330.10289883170691426No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT14330.10289883170691426No Hit
GAGTTATAACTTCATCTCCTGGTACCTACATCACCCCGGCCAAGCCCCCAAAGCCCTGATTTATGAGGTCAATCA14280.10253979879795783No Hit
CTTCTCCACGGTACTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14150.10160631323467108No Hit
GACTGAGGACGAGGGTCATTATTATTGTTGCTCATATGTAACTGGTAGGAAATTGGTGTTCGGCGGAGGGACCA14100.10124728032571464No Hit
GTAATAAACGGCCGTGTCGTCAGAACGCAGGCTGGTCACCTCCATGTAGGCCGTGTTCATGGAGATGTCCCTGGT14060.10096005399854951No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)