FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1392630
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCAATTTCCTACCA64920.4661683289890351No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC64600.46387051837171395No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG60410.4337835606011647No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCAATTTCCTACCAGTTACATATGAGCA59990.43076768416593064No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAGGCAACAGTGATGTTGGGAGTTATAACTTCATCTCCTGGT57180.41059003468257904No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT53580.3847396652377157No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA49950.3586738760474785No Hit
CTCCTGCACTGGAGGCAACAGTGATGTTGGGAGTTATAACTTCATCTCCTGGTACCTACATCACCCCGGCCAAGC45710.32822788536797287No Hit
GTACTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT45580.3272943998046861No Hit
TTATTATTGTTGCTCATATGTAACTGGTAGGAAATTGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTC44760.3214062600978006No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG42470.3049625528675958No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC39820.2859338086929048No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC38370.2755218543331682No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC38070.27336765687942954No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38070.27336765687942954No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTACTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT36520.2622376367017801No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC34630.24866619274322685No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG34520.24787632034352267No Hit
GTCTGGAGCCCAGAAATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC34050.24450141099933217No Hit
GTCTTCTCCACGGTACTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC33560.24098288849155913No Hit
GTCGATCACCATCTCCTGCACTGGAGGCAACAGTGATGTTGGGAGTTATAACTTCATCTCCTGGTACCTACATC33030.2371771396566209No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC31650.2272678313694233No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC31560.22662157213330175No Hit
GTATTCAGGGGTTTCTAACCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATTTCTGGGCTCC31120.22346208253448513No Hit
ATTCAGGGGTTTCTAACCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATTTCTGGGCTCC31000.22260040355298966No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC30860.22159511140791166No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC30210.21692768359147802No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG30010.21549155195565226No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG29820.21412722690161778No Hit
ACATCACCCCGGCCAAGCCCCCAAAGCCCTGATTTATGAGGTCAATCAACGGTATTCAGGGGTTTCTAACCGCTT29810.21405542031982652No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG29790.21391180715624394No Hit
CAATAATAATGACCCTCGTCCTCAGTCTGGAGCCCAGAAATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC29250.21003425173951443No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC29180.20953160566697543No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG28610.20543863050487207No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCAATTTCCTACCAGTTACATATGAGCAACAATAATAATG28420.20407430545083763No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT27900.2003403631976907No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA27450.19710906701708278No Hit
GCTCCAGACTGAGGACGAGGGTCATTATTATTGTTGCTCATATGTAACTG27050.19423680374543129No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA27000.19387777083647487No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC26660.1914363470555711No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC26350.18921034302004122No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC26340.18913853643824993No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGG26160.18784601796600675No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA26110.18748698505705033No Hit
CCTTGGTCCCTCCGCCGAACACCAATTTCCTACCAGTTACATATGAGCAA24940.17908561498746975No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24400.17520805957074026No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTG24260.17420276742566224No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC24050.17269482920804521No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG23320.16745294873728125No Hit
GATTTATGAGGTCAATCAACGGTATTCAGGGGTTTCTAACCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTC23240.16687849608295097No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACC22730.16321636041159532No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC22680.1628573275026389No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA22250.15976964448561354No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAGGCA22020.15811809310441396No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT21900.15725641412291852No Hit
CCCCTGAATACCGTTGATTGACCTCATAAATCAGGGCTTTGGGGGCTTGGCCGGGGTGATGTAGGTACCAGG21630.15531763641455376No Hit
GGGTGATGTAGGTACCAGGAGATGAAGTTATAACTCCCAACATCACTGTT21340.15323524554260642No Hit
CTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC20830.1495731098712508No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA20610.1479933650718425No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC20580.14777794532646862No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC19890.14282329118286982No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACA19850.14253606485570466No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTACTCCCTTCATGCGTGACCTGGC19600.1407409003109225No Hit
GTTACATATGAGCAACAATAATAATGACCCTCGTCCTCAGTCTGGAGCCCAGAAATTGTCAGGGAGGCCGTGTTG19400.13930476867509675No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC19350.13894573576614033No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCAATTTCCTAC18650.13391927504075024No Hit
GGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT18590.13348843555000253No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18330.13162146442342904No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG18250.13104701176909875No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC18070.12975449329685557No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA17960.12896462089715144No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGG17870.12831836166102983No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC17730.12731306951595184No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACAC17000.12207118904518789No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT16980.12192757588160531No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCCCAGGACCCTGTGTCCTGAGTGAGG16870.12113770348190114No Hit
TATGTAACTGGTAGGAAATTGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC16870.12113770348190114No Hit
GTTAGAAACCCCTGAATACCGTTGATTGACCTCATAAATCAGGGCTTTGGGGGCTTGGCCGGGGTGATGTAGGT16620.11934253893711898No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGG16360.11747556781054552No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGC16310.11711653490158909No Hit
ACGGTATTCAGGGGTTTCTAACCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATTTCTGGGCT16040.11517775719322434No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA16000.11489053086605919No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA15950.11453149795710275No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTACTCCCTTCATGCGTGACC15900.11417246504814632No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC15660.11244910708515543No Hit
GCCCTGATTTATGAGGTCAATCAACGGTATTCAGGGGTTTCTAACCGCTT15600.11201826759440771No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCAC15580.11187465443082513No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC15490.11122839519470354No Hit
GTATCAACGCAGAGTACGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTG15480.11115658861291226No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTACTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCC15450.1109411688675384No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGC15340.11015129646783424No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC15150.10878697141379978No Hit
ACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG15050.10806890559588692No Hit
ACCTACATCACCCCGGCCAAGCCCCCAAAGCCCTGATTTATGAGGTCAAT15030.10792529243230434No Hit
TCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCC15020.10785348585051306No Hit
CCTGAATACCGTTGATTGACCTCATAAATCAGGGCTTTGGGGGCTTGGCCGGGGTGATGTAGGTACCAGGAGATG14970.10749445294155663No Hit
GAATACCGTTGATTGACCTCATAAATCAGGGCTTTGGGGGCTTGGCCGGG14910.10706361345080889No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC14890.10692000028722634No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTACTCCCTTCATGCGTGACCTGGC14590.10476580283348771No Hit
ATATGTAACTGGTAGGAAATTGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC14590.10476580283348771No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACC14570.10462218966990514No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACAC14530.10433496334274No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCC14500.10411954359736614No Hit
GACTGAGGACGAGGGTCATTATTATTGTTGCTCATATGTAACTGGTAGGAAATTGGTGTTCGGCGGAGGGACCA14500.10411954359736614No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGTACCGTGGAGAAGACAGTGGCCCCTAC14450.1037605106884097No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT14290.1026116053797491No Hit
TCCTCAGTCTGGAGCCCAGAAATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGGTTAGAAACC14200.10196534614362751No Hit
GTAATAAACGGCCGTGTCGTCAGAACGCAGGCTGGTCACCTCCATGTAGGCCGTGTTCATGGAGATGTCCCTGGT14080.10110366716213207No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)