FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1310524
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC39640.3024744300752981No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG34270.2614984540534931No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG32300.24646629897659258No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCC32020.24432974901642396No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCAGCACGCAGGCTGTTATCCCACGTTGCGCAGTAAT31900.24341408474778028No Hit
GTAATAATCGGCCTCGTCTCCAGTCTGGAGATCGGTGATGCCCAGAGTGGCTGACGTGCCAGACTTGGAGCCAG29590.2257875475763893No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28830.21998834054164593No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27510.2099160335865654No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTATGACAATAATAGGCGACCCTCAGGGATTCCTGACCGATTCTCTGG27240.20785578898211707No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA26400.20144613910161127No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC25130.19175535892513224No Hit
GATTATTACTGCGCAACGTGGGATAACAGCCTGCGTGCTGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCT22130.16886375220904004No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21930.1673376450946339No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGT21800.1663456754702699No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTC21680.16543001120162623No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG21670.16535370584590592No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC21540.1643617362215419No Hit
CCCCAGCACGCAGGCTGTTATCCCACGTTGCGCAGTAATAATCGGCCTCGTCTCCAGTCTGGAGATCGGTGATGC21410.16336976659717792No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC20610.15726533813955335No Hit
GTTCCTGGGAGATGCTGGTACCAGGATACATAATTATTACCAATGTTGGAGCTGCTTCCAGAGCAGGAGATGGTG20400.15566292566942688No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT19230.14673519905015092No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19160.14620106156010879No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC19030.1452090919357448No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18630.1421568777069325No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAGTGGA18430.14063077059252635No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC18290.13956249561244205No Hit
GGATAACAGCCTGCGTGCTGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC18060.137807472430875No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG17720.13521309033638454No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17160.1309399904160473No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATG16520.12605644764994767No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT16070.12262270664253382No Hit
GGGCATCACCGATCTCCAGACTGGAGACGAGGCCGATTATTACTGCGCAA16050.12247009593109322No Hit
CTGTTATCCCACGTTGCGCAGTAATAATCGGCCTCGTCTCCAGTCTGGAG15800.12056246203808554No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15560.11873113350079816No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15510.1183496067221966No Hit
GTGCTGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC15120.11537369784910463No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCAGCACGCAGGCTGTTATCCCACGTTGCGCAGTAATA14930.1139238960904188No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14720.11232148362029235No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG14670.11193995684169081No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC14560.11110059792876742No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGTAATAATTATGTATCCTGGTACCAGCAT14500.11064276579444557No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14430.11010862830440342No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC14260.10881143725715821No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC14200.10835360512283636No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT13730.10476725340398192No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC13680.10438572662538037No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13240.10102829097368687No Hit
GTGATGCCCAGAGTGGCTGACGTGCCAGACTTGGAGCCAGAGAATCGGTC13200.10072306955080564No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)