FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1310524
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42560.32475559394562786No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC36710.2801169608492481No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG30700.23425744206134338No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG29110.22212489050181453No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA26370.20121722303445033No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26110.19923328378572236No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTATGACAATAATAGGCGACCCTCAGGGATTCCTGACCGATTCTCTGG25260.19274732854949625No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCAGCACGCAGGCTGTTATCCCACGTTGCGCAGTAAT24980.19061077858932762No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24910.19007664109928546No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCC24380.1860324572461092No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTG23420.1787071430969597No Hit
GTAATAATCGGCCTCGTCTCCAGTCTGGAGATCGGTGATGCCCAGAGTGGCTGACGTGCCAGACTTGGAGCCAG22990.17542601280098646No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC22580.17229749321645388No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC22320.17031355396772588No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGT22290.17008463790056497No Hit
GATTATTACTGCGCAACGTGGGATAACAGCCTGCGTGCTGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCT22130.16886375220904004No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCTGGAAGCAGCTCC20740.15825730776391733No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG20600.15718903278383303No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20010.1526870167963349No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTC19820.15123721503764906No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC19810.15116090968192875No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17460.13322915108765654No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17400.13277131895333472No Hit
GTGCTGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC17130.1307110743488864No Hit
GGATAACAGCCTGCGTGCTGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC17000.12971910472452242No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC16760.127887776187235No Hit
GTTCCTGGGAGATGCTGGTACCAGGATACATAATTATTACCAATGTTGGAGCTGCTTCCAGAGCAGGAGATGGTG16420.1252933940927446No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAGTGGA16310.1244540351798212No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT15680.11964679776944184No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG15540.11857852278935754No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATG15330.1169761103192311No Hit
CCCCAGCACGCAGGCTGTTATCCCACGTTGCGCAGTAATAATCGGCCTCGTCTCCAGTCTGGAGATCGGTGATGC15270.11651827818490924No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15110.11529739249338433No Hit
GGGCATCACCGATCTCCAGACTGGAGACGAGGCCGATTATTACTGCGCAA14940.11400020144613911No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG14640.11171104077452988No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14570.11117690328448773No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC14490.11056646043872528No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC14330.10934557474720034No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGTAATAATTATGTATCCTGGTACCAGCAT13850.10568291767262561No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13110.10003632134932287No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)