FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1307469
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC38620.2953798522182935No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG35070.2682281568434892No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32050.2451300948626698No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31860.2436769055327507No Hit
GGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCAGCACTGCTTGGGTGTTCGGCAGAGGGACCA27430.20979464905095266No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA26840.2052821137633091No Hit
TCCATAGCCTGGGTGCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATC25820.19748078157111182No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC25690.19648649413485136No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC24910.19052076951728875No Hit
GTAATAGACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGCGTTCTTGGCGTTGTCTCTGG23980.18340779016557945No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC23410.17904822217582214No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC23160.17713613095224437No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG22770.17415326864346306No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT22340.1708644717389093No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT22190.16971721700476264No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC22180.16964073335581953No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCTGCCGAACACCCAAGCAGTGCTGCTGTCCCACGCCTGACAGTAATAGT21910.16757567483435554No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG20890.15977434264215826No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCTGCCGAACACCCAAGCAGTGCTGCTGTCCCACGCCTG20730.15855060425906847No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC20660.1580152187164667No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCT20430.15625609479077515No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC20310.15533829100345783No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA19930.15243191234361964No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19390.14830179530069162No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18580.14210661973629968No Hit
GACTATTACTGTCAGGCGTGGGACAGCAGCACTGCTTGGGTGTTCGGCAGAGGGACCAAGCTGACCGTCCTAGGT18500.1414947505447548No Hit
CTATTACTGTCAGGCGTGGGACAGCAGCACTGCTTGGGTGTTCGGCAGAGGGACCAAGCTGACCGTCCTAGGTC17840.13644682971450947No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG17710.13545254227824904No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC17340.13262264726735395No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTT17340.13262264726735395No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCACCATCACCTGCTCTGG17320.13246967996946774No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG17180.13139890888426417No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17010.1300986868522313No Hit
CCCCAGGACAGACAGCCACCATCACCTGCTCTGGAGATAAATTGGGGAATAAATATACTTCCTGGTATCAGCAGA16970.12979275225645887No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16250.12428592953255488No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16170.12367406034101No Hit
AGCCAGGCCAGTCCCCTGTGTTTGTCATCTATCGAGATAACAAACGCCCC15910.12168548546848913No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC15800.12084416533011491No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15600.11931449235125267No Hit
GTCTATTACTGTGCGAGAGAATGGGCTCATTGTAATGATACCACTTGCTGGCGCTACGGTATGGACGTCTGGGGC15180.11610217909564204No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC14620.11181909475482783No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14520.11105425826539674No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT14320.10952458528653451No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14230.10883623244604652No Hit
GAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGGCGTTTGTTATCTCGAT14080.10768897771189986No Hit
CGTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCACCATCACCTGCTCTGGAGATA14080.10768897771189986No Hit
CAGTAATAGTCAGCCTCATCCATAGCCTGGGTGCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTTGGAGCC13920.10646523932881011No Hit
CAGCAGCACTGCTTGGGTGTTCGGCAGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT13790.10547095189254965No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13790.10547095189254965No Hit
CAGCCACTCTGACCATCAGCGGCACCCAGGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCAGC13730.105012049998891No Hit
CCCAAGCAGTGCTGCTGTCCCACGCCTGACAGTAATAGTCAGCCTCATCCATAGCCTGGGTGCCGCTGATGGTC13700.10478259905206165No Hit
GTAATAGTCAGCCTCATCCATAGCCTGGGTGCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTTGGAGCCAG13690.10470611540311855No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT13400.10248808958376833No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA13150.10057599836019056No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)