FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1264372
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCACTTTGGTCCCACCGCCGAAAAGTACATAAGCGGAGTAGCCTGCAAATGAGCAGCAGAAAT58710.46434119072551433No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCATGCCAACAAGG54990.434919469902845No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC52360.41411862964380736No Hit
GCCTTGGGCTGACCTAGGACGGTCACTTTGGTCCCACCGCCGAAAAGTACATAAGCGGAGTAGCCTGCAAATG45970.3635797059726093No Hit
CCCTTACCATCTCGGGGCTCCAGACTGAGGATGCGGCTGATTATTTCTGCTGCTCATTTGCAGGCTACTCCGCTT40020.31652077078581303No Hit
CCTCTGAGGAGCTTCATGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39810.3148598671909849No Hit
GTGTCCGGGTCTCCTGGGCAGTCAGTCACCATTTCCTGCACTGGAACCAG37300.2950081147004205No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA36250.28670359672627993No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32160.2543555219508183No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC31850.2519037118822625No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT28660.22667379537034985No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACTTTGGTCCCAC28460.22509198242289452No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG27030.21378201984858886No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT26670.21093475654316926No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC26130.20666386158503985No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCATGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG26010.2057147738165666No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCAGTCAGGGCACAGGATCCTGGG25770.20381659827962023No Hit
GTCTGGAGCCCCGAGATGGTAAGGGAGGCCGTGTTGCCAGACTTGGAGCC25200.19930843137937254No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC24920.19709389325293503No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC24110.19068755081574093No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC24050.19021300693150434No Hit
ACCTAGGACGGTCACTTTGGTCCCACCGCCGAAAAGTACATAAGCGGAGTAGCCTGCAAATGAGCAGCAGAAATA23840.18855210333667624No Hit
GCTCCAGACTGAGGATGCGGCTGATTATTTCTGCTGCTCATTTGCAGGCT23700.1874448342734575No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGGCAGTCAGTCACCATTTCCTG23570.18641665585761152No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG23330.18451848032066512No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCATGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23130.1829366673732098No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23020.18206667025210935No Hit
CTCCTGGGCAGTCAGTCACCATTTCCTGCACTGGAACCAGCAGTGATGTTGGAAGTTATGAATATGTGTCTTGGT22660.17921940694668975No Hit
CACCAGTGTGGCCTTGTTGGCATGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21930.17344578968847776No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21400.1692539853777211No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA21330.16870035084611174No Hit
GTACATAAGCGGAGTAGCCTGCAAATGAGCAGCAGAAATAATCAGCCGCA20810.16458763718272787No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG20200.15976310769298907No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCATGAAGCTCCTCAGAGGA20110.1590512918666342No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGGCAGTCAGTCACCATTT19860.15707402568231502No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCAGTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC19430.15367312784528603No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGGCAGTCAGTCAC18950.14987677677139324No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTG18460.14600133505012763No Hit
TGGTAAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGGTCAGGGACGCCTGAGGGCCTCTTAGTGACGT18260.1444195221026723No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC18180.1437867969236902No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCATGA18110.1432331623920808No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC17490.13832954225496927No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGGCAGTCAGTCAC17390.1375386357812416No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACTTTGGTCCCACCGCCGAAAAGTACAT17390.1375386357812416No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16770.13263501564413005No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16510.13057865881243813No Hit
TCCTCAGCCTCCTCAGTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGT16320.12907593651235555No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC16310.12899684586498278No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16090.1272568516227819No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGGCAGTCAGTCACCATTTCCTGCA15970.1263077638543087No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACA15550.1229859566646525No Hit
GTCCTGGACCTCCCGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC15460.12227414083829759No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15380.12164141565931545No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC15350.12140414371719715No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC15260.12069232789084225No Hit
TTCCTGCACTGGAACCAGCAGTGATGTTGGAAGTTATGAATATGTGTCTTGGTACCAATTTCACCCAGGAGCAGC14980.11847778976440478No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACTTTGGTCCCACCGCCGAAAAGTACATAAGCGG14070.111280540853483No Hit
CTTATGTACTTTTCGGCGGTGGGACCAAAGTGACCGTCCTAGGTCAGCCC14030.11096417826399192No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC14020.11088508761661917No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCAGTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACT14000.11072690632187361No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13940.11025236243763703No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13740.10867054949018169No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCC13730.10859145884280891No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCATGCCAACAAGGCC13670.10811691495857233No Hit
CAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGGCAGTCAGTCACCATTTCCTGCACTGGAACC13390.10590237683213485No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT13320.10534874230052549No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT13310.1052696516531527No Hit
CTTTGGTCCCACCGCCGAAAAGTACATAAGCGGAGTAGCCTGCAAATGAGCAGCAGAAATAATCAGCCGCATCCT13010.1028969322319697No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC12920.10218511640561481No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12830.10147330057925989No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12820.10139420993188714No Hit
CCTGAGGGCCTCTTAGTGACGTCATAAAGAATGAGTTTGGGGGCTGCTCC12680.1002869408686684No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA12680.1002869408686684No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12660.10012875957392285No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)