FastQCFastQC Report
Wed 8 May 2024
EGAF00004717739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences744019
Total Bases55.1 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACT48980.6583165214866825No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA46050.6189358067468708No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT41440.5569750234873034No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA40830.548776308131916No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC40540.5448785582088629No Hit
GACCTAGGACGATGACGTTGGTCCCACTTCCGAAGACATAAGCACTGAGG39960.5370830583627569No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35550.4778103784983985No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC35030.47082130967085517No Hit
ACTTATTACTGCCAGTCCTATGACAACAGCCTCAGTGCTTATGTCTTCGG34200.45966568058073787No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT33400.44891326699990186No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC31290.420553776180447No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC29520.39676406113284746No Hit
GATGTACACTGGTACCAGCACCTTCCAGGATCAGCCCCCAAACTCTTCAT27860.37445280295261274No Hit
GGTTATGATGTACACTGGTACCAGCACCTTCCAGGATCAGCCCCCAAACT27090.36410360488105814No Hit
GCCTTGGGCTGACCTAGGACGATGACGTTGGTCCCACTTCCGAAGACATA26500.3561736998651916No Hit
CATAAGCACTGAGGCTGTTGTCATAGGACTGGCAGTAATAAGTAGCCCCA24670.3315775537990293No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGATGACGTTGGTC24060.3233788384436419No Hit
ACCTAGGACGATGACGTTGGTCCCACTTCCGAAGACATAAGCACTGAGGC23870.32082514021819336No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG23540.31638976961609855No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC23390.3143736920696918No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22740.30563735603526254No Hit
GTAATAAGTAGCCCCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG22710.3052341405259812No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA22230.2987826923774796No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT21630.2907183821918526No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21610.2904495718523317No Hit
TTCCAGGATCAGCCCCCAAACTCTTCATCTATGCTAATAACAATCGGCCC21580.29004635634305037No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC21360.2870894426083205No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT21090.28346050302478837No Hit
GTCCTATGACAACAGCCTCAGTGCTTATGTCTTCGGAAGTGGGACCAACG20870.2805035892900585No Hit
GTGCTTATGTCTTCGGAAGTGGGACCAACGTCATCGTCCTAGGTCAGCCC20830.27996596861101664No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC20250.2721704687649106No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGGGGCTACTTATTACTGCCAGT19920.26773509816281577No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGC19860.26692866714425306No Hit
GTCATAGGACTGGCAGTAATAAGTAGCCCCATCCTCAGCCTGGAGCCCAG18950.25469779669605214No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGT18620.25026242609395727No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG18560.2494559950753946No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG17920.24085406421072578No Hit
CTCTTCATCTATGCTAATAACAATCGGCCCTCAGCGGTCCCTGACCGATT17480.23494023674126602No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT17140.23037046096941075No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGT16970.22808557308348307No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC16840.22633830587659726No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG16170.2173331595026471No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC15990.214913866446959No Hit
TCACTGGGCTCCAGGCTGAGGATGGGGCTACTTATTACTGCCAGTCCTAT15690.21088171135414552No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14750.19824762539666324No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC14680.19730678920834013No Hit
GTCAGGGACCGCTGAGGGCCGATTGTTATTAGCATAGATGAAGAGTTTGG14570.19582833234097516No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA14410.19367784962480797No Hit
GCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCACCTTCCA13960.18762961698558775No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCT13910.1869575911367855No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13590.1826566257044511No Hit
GCCCTCAGCGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG13500.18144697917660704No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC13290.1786244706116376No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG13240.17795244476283537No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG13000.17472672068858458No Hit
AAGTAGCCCCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAG12950.1740546948397823No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTG12910.17351707416074053No Hit
ATGCTAATAACAATCGGCCCTCAGCGGTCCCTGACCGATTCTCTGGCTCC12840.1725762379724174No Hit
GTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCT12820.17230742763289647No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG12660.17015694491672928No Hit
GTACCAGCACCTTCCAGGATCAGCCCCCAAACTCTTCATCTATGCTAATA12550.16867848804936433No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATG12500.1680064622005621No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT12410.16679681567271804No Hit
GGATGGGGCTACTTATTACTGCCAGTCCTATGACAACAGCCTCAGTGCTT12350.16599038465415533No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG12320.16558716914487398No Hit
GTGTACATCATAACCTGCCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGA12190.16383990193798814No Hit
GTGCTGGTACCAGTGTACATCATAACCTGCCCCGATGTTGGAGCTGCTCC12060.1620926347311023No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTG12010.16142060888230006No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC11990.16115179854277914No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC11980.1610173933730187No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG11800.1585981003173306No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCC11710.15738845378948657No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11650.15658202277092384No Hit
GATGAAGAGTTTGGGGGCTGATCCTGGAAGGTGCTGGTACCAGTGTACAT11650.15658202277092384No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCA11520.154834755564038No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA11520.154834755564038No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAG11510.15470035039427757No Hit
CCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGG11470.15416272971523576No Hit
GTTATGATGTACACTGGTACCAGCACCTTCCAGGATCAGCCCCCAAACTC11310.15201224699906857No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGG11110.1493241436038596No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG11060.14865211775505735No Hit
TCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTT10950.14717366088769238No Hit
CCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATC10910.1466360402086506No Hit
GCTCCAGGCTGAGGATGGGGCTACTTATTACTGCCAGTCCTATGACAACA10880.14623282469936924No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG10750.1444855574924834No Hit
ATCCTGGAAGGTGCTGGTACCAGTGTACATCATAACCTGCCCCGATGTTG10720.14408234198320205No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGT10560.14193185926703486No Hit
GCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAAT10510.14125983341823262No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTC10500.14112542824847213No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCGCTGAGGGCCGATT10430.14018459206014902No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCC10420.14005018689038853No Hit
ACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGC10330.1388405403625445No Hit
GGGCTACTTATTACTGCCAGTCCTATGACAACAGCCTCAGTGCTTATGTC10320.13870613519278405No Hit
GTTGTCATAGGACTGGCAGTAATAAGTAGCCCCATCCTCAGCCTGGAGCC10300.13843732485326316No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGC10260.13789970417422134No Hit
GGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCC10170.13669005764637732No Hit
GGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTAT10050.1350771956092519No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG10020.13467398009997056No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC9940.13359873874188696No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC9830.13212028187452202No Hit
ATGATGTACACTGGTACCAGCACCTTCCAGGATCAGCCCCCAAACTCTTC9800.13171706636524066No Hit
TACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTT9790.1315826611954802No Hit
GACATAAGCACTGAGGCTGTTGTCATAGGACTGGCAGTAATAAGTAGCCC9750.13104504051643842No Hit
GCTTATGTCTTCGGAAGTGGGACCAACGTCATCGTCCTAGGTCAGCCCAA9660.12983539398859437No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTC9580.12876015263051077No Hit
GGTACCAGCACCTTCCAGGATCAGCCCCCAAACTCTTCATCTATGCTAAT9580.12876015263051077No Hit
GGCTGAGGATGGGGCTACTTATTACTGCCAGTCCTATGACAACAGCCTCA9490.12755050610266674No Hit
CAGCGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC9420.12660966991434358No Hit
CAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGG9410.12647526474458315No Hit
GCCCCCAAACTCTTCATCTATGCTAATAACAATCGGCCCTCAGCGGTCCC9350.12566883372602042No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGG9330.12540002338649955No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA9260.12445918719817639No Hit
GCCCCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCC9260.12445918719817639No Hit
TCCAGGATCAGCCCCCAAACTCTTCATCTATGCTAATAACAATCGGCCCT9120.1225775148215301No Hit
CCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATGGGGCTACT9070.12190548897272784No Hit
CACTGGTACCAGCACCTTCCAGGATCAGCCCCCAAACTCTTCATCTATGC9070.12190548897272784No Hit
CATCATAACCTGCCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGATGGTG9050.12163667863320696No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG9050.12163667863320696No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGA8920.11988941142632109No Hit
GACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGG8880.1193517907472793No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC8790.11814214421943525No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG8770.11787333387991436No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGA8720.1172013080311121No Hit
CTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGA8670.11652928218230986No Hit
AGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGA8660.11639487701254941No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA8630.11599166150326806No Hit
ATGTTGGAGCTGCTCCCAGTGCAGGAGATGGTGACCCTCTGCCCTGGGGC8560.1150508253149449No Hit
TACCAGCACCTTCCAGGATCAGCCCCCAAACTCTTCATCTATGCTAATAA8460.11370677361734043No Hit
TCCCTGGCCATCACTGGGCTCCAGGCTGAGGATGGGGCTACTTATTACTG8350.11222831674997547No Hit
GGGTTGGCCTTGGGCTGACCTAGGACGATGACGTTGGTCCCACTTCCGAA8320.11182510124069411No Hit
GGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGA8320.11182510124069411No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCA8230.11061545471285007No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAG8140.10940580818500603No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTC8130.10927140301524559No Hit
ACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGATGACGTTGGTCCCACT8120.10913699784548513No Hit
GGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGAT8040.10806175648740153No Hit
CCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACA7930.10658329962003657No Hit
CAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGC7910.10631448928051569No Hit
GTTTGGGGGCTGATCCTGGAAGGTGCTGGTACCAGTGTACATCATAACCT7910.10631448928051569No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7890.10604567894099477No Hit
CTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCAT7860.10564246343171345No Hit
CACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCC7760.10429841173410893No Hit
GCTAATAACAATCGGCCCTCAGCGGTCCCTGACCGATTCTCTGGCTCCAA7740.10402960139458806No Hit
CAGTGGGGTTGGCCTTGGGCTGACCTAGGACGATGACGTTGGTCCCACTT7700.10349198071554624No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7680.10322317037602534No Hit
GTACACTGGTACCAGCACCTTCCAGGATCAGCCCCCAAACTCTTCATCTA7610.10228233418770219No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA7600.10214792901794174No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA7580.10187911867842085No Hit
GTCAATGAGGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGG7510.1009382824900977No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAAC7500.10080387732033726No Hit
GTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCAC7500.10080387732033726No Hit
CATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCC7490.1006694721505768No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT7460.10026625664129546No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)