FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences744019
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCACCTTCCAGGATCAGC42110.5659801698612535No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT35760.480632887063368No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC34420.4626225943154678No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGATGACGTTGGTCCC32810.44098336198403537No Hit
ACTTATTACTGCCAGTCCTATGACAACAGCCTCAGTGCTTATGTCTTCGGAAGTGGGACCAACGTCATCGTCCT32230.43318786213792926No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG28390.3815762769499166No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG28120.3779473373663844No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC27840.3741839926130919No Hit
GACCTAGGACGATGACGTTGGTCCCACTTCCGAAGACATAAGCACTGAGGCTGTTGTCATAGGACTGGCAGTAAT26610.35765215673255657No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTC25370.34098591568226083No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC24950.3353408985523219No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC24860.33413125202447785No Hit
GATGTACACTGGTACCAGCACCTTCCAGGATCAGCCCCCAAACTCTTCATCTATGCTAATAACAATCGGCCCTC24550.3299646917619039No Hit
GGTTATGATGTACACTGGTACCAGCACCTTCCAGGATCAGCCCCCAAACTCTTCATCTATGCTAATAACAATCGG22980.3088630801095133No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG22300.29972352856580275No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGT20510.27566500317868226No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC20190.2713640377463479No Hit
GCCTTGGGCTGACCTAGGACGATGACGTTGGTCCCACTTCCGAAGACATAAGCACTGAGGCTGTTGTCATAGG19920.26773509816281577No Hit
TTCCAGGATCAGCCCCCAAACTCTTCATCTATGCTAATAACAATCGGCCC19880.2671974774837739No Hit
GTGCTTATGTCTTCGGAAGTGGGACCAACGTCATCGTCCTAGGTCAGCCC19650.2641061585792836No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG19520.26235889137239776No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTC19500.26209008103287684No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCC19480.2618212706933559No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC18840.25321933982868716No Hit
GTCCTATGACAACAGCCTCAGTGCTTATGTCTTCGGAAGTGGGACCAACGTCATCGTCCTAGGTCAGCCCAAGGC18460.24811194337779008No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC18150.24394538311521613No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17670.23749393496671456No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGGGGCTACTTATTACTGCCAGT17620.23682190911791232No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGATGACGTTGGTCCCACTTCCGAAGACATAAGCACTG17370.2334617798739011No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT17210.2313112971577339No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT17040.2290264092718062No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGG16500.22176853010474196No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACC16490.22163412493498147No Hit
ACCTAGGACGATGACGTTGGTCCCACTTCCGAAGACATAAGCACTGAGGCTGTTGTCATAGGACTGGCAGTAATA16110.21652672848408444No Hit
CTCTTCATCTATGCTAATAACAATCGGCCCTCAGCGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTC15940.21424184059815674No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG15450.20765598727989473No Hit
CATAAGCACTGAGGCTGTTGTCATAGGACTGGCAGTAATAAGTAGCCCCATCCTCAGCCTGGAGCCCAGTGATGG15120.2032206166777999No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT15060.2024141856592372No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGG14890.20012929777330954No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTG14760.19838203056642373No Hit
TCACTGGGCTCCAGGCTGAGGATGGGGCTACTTATTACTGCCAGTCCTAT14690.19744119437810057No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGA14540.19542511683169383No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG14430.19394665996432886No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTG14140.19004891004127583No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC13880.18655437562750413No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT13770.18507591876013918No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13690.18400067740205558No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC13530.1818501946858884No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGG13370.1796997119697212No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC13130.17647398789547042No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC13090.1759363672164286No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATG12980.17445791034906366No Hit
GTAATAAGTAGCCCCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAG12660.17015694491672928No Hit
GTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGT12530.16840967770984344No Hit
GTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGC12230.16437752261702993No Hit
GTCATAGGACTGGCAGTAATAAGTAGCCCCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCC12220.1642431174472695No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT12040.16182382439158138No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC11810.15873250548709106No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTG11760.15806047963828881No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11730.15765726412900746No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11660.15671642794068433No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG11460.15402832454547533No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC11320.15214665216882903No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACA11120.14945854877362003No Hit
ATGCTAATAACAATCGGCCCTCAGCGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG11110.1493241436038596No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC11070.14878652292481778No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG10870.14609841952960878No Hit
CTCACTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCA10810.14529198851104608No Hit
GGATGGGGCTACTTATTACTGCCAGTCCTATGACAACAGCCTCAGTGCTTATGTCTTCGGAAGTGGGACCAACGT10810.14529198851104608No Hit
GGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGC10630.142872695455358No Hit
GTACCAGCACCTTCCAGGATCAGCCCCCAAACTCTTCATCTATGCTAATAACAATCGGCCCTCAGCGGTCCCTG10590.1423350747763162No Hit
CCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGT10520.14139423858799305No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10370.1393781610415863No Hit
GCCCTCAGCGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCC10210.1372276783254191No Hit
CCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGG10010.13453957493021013No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCC9910.13319552323260564No Hit
GCTCCAGGCTGAGGATGGGGCTACTTATTACTGCCAGTCCTATGACAACA9790.1315826611954802No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC9760.13117944568619888No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT9700.13037301466763618No Hit
GTTATGATGTACACTGGTACCAGCACCTTCCAGGATCAGCCCCCAAACTCTTCATCTATGCTAATAACAATCGGC9490.12755050610266674No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG9420.12660966991434358No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCC9380.1260720492353018No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC9300.12499680787721819No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG9240.1241903768586555No Hit
GGGCTACTTATTACTGCCAGTCCTATGACAACAGCCTCAGTGCTTATGTC9210.12378716134937415No Hit
CAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGG9180.1233839458400928No Hit
GGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTC9170.12324954067033235No Hit
ACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG9040.12150227346344651No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT8980.1206958424448838No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGA8960.12042703210536289No Hit
GGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCACCTTC8940.120158221765842No Hit
GTCAGGGACCGCTGAGGGCCGATTGTTATTAGCATAGATGAAGAGTTTGGGGGCTGATCCTGGAAGGTGCTGGT8900.1196206010868002No Hit
GCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCACCTTCCAGGATCAGCCCCCAAACTCTTCATCT8820.1185453597287166No Hit
ATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTG8710.11706690286135166No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC8680.1166636873520703No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG8660.11639487701254941No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC8620.11585725633350762No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGA8560.1150508253149449No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAG8480.1139755839568613No Hit
TACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTT8380.11263153225925682No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCC8310.11169069607093367No Hit
ATGATGTACACTGGTACCAGCACCTTCCAGGATCAGCCCCCAAACTCTTCATCTATGCTAATAACAATCGGCCCT8250.11088426505237098No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC8220.11048104954308963No Hit
CCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATGGGGCTACTTATTACTGCCAGTCCTATGACAAC8120.10913699784548513No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAA8090.10873378233620377No Hit
CAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCAC8090.10873378233620377No Hit
GTATCAACGCAGAGTACGGGAGGCTCTGCTTCAGCTGTGGGCACAAGAGG8060.10833056682692244No Hit
TCCAGGATCAGCCCCCAAACTCTTCATCTATGCTAATAACAATCGGCCCTCAGCGGTCCCTGACCGATTCTCTGG8040.10806175648740153No Hit
AAGTAGCCCCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAG8030.10792735131764108No Hit
TCCCTGGCCATCACTGGGCTCCAGGCTGAGGATGGGGCTACTTATTACTG7970.10712092029907837No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAACAG7910.10631448928051569No Hit
GGCTGAGGATGGGGCTACTTATTACTGCCAGTCCTATGACAACAGCCTCAGTGCTTATGTCTTCGGAAGTGGGAC7830.10523924792243208No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC7690.10335757554578578No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCC7680.10322317037602534No Hit
CTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGG7670.10308876520626489No Hit
AGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGC7670.10308876520626489No Hit
GGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC7510.1009382824900977No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGG7480.10053506698081635No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)