FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences745211
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTACCCCTCACCTTCGGCCAAGGGACACGGCTGGAGATTAAA39930.5358213982348623No Hit
CCTATGTGCTGACTCAGCCACCGTCGGTGTCAGTGGCCCCAGGACAGACGGCCTGGATTTCGTGTGGGGGAAACA22090.29642611287273No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAGACCACCCAAGGATCACCACTACTATCCCACACCTGACAGT21870.2934739288604167No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20910.2805916713521405No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20490.2749556836922697No Hit
CTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGGCCGTG18900.2536194446941873No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18310.24570222393389254No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18090.24275003992157929No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA17050.22879426095428007No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT16810.22557369657721102No Hit
CCCCAGGACAGACGGCCTGGATTTCGTGTGGGGGAAACAACATTGCAATTAAAAGTGTGCACTGGTACCAGCAGA16770.2250369358476995No Hit
CTATAATACTGTCGACAGTAACAAGATGCAAAATCTTCAGGCTGCAGGCCGCTGATGGTGAGAGTGTAATCCGTC16680.2238292242062986No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGAGTTCTTGGTGTTGTCTCTGG16160.216851334722649No Hit
GGATAGTAGTGGTGATCCTTGGGTGGTCTTCGGCGGAGGGACCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTGC16060.21550943289887023No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15740.21121534706277817No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15100.20262717539059408No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAGACCACCCAAGGATCACCACTACTATCCCAC14620.196186046636456No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTC14510.19470995463029933No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC13750.1845115007695807No Hit
GGGTGGCCGTGCTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCGATCATCAA12700.17042153161990362No Hit
AGTCAGGACACAGCATGGACATGAGGGTGCCCGCTCAGCGCCTGGGGCTCCTGCTGCTCTGGTTCCCAGTTTGC12680.17015315125514788No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12650.16975058070801424No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG12510.16787191815472396No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT12470.16733515742521246No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCGTCGGTGTCAGTGGCCCCAGGAC12460.16720096724283456No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC12310.16518811450716644No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCC12160.16317526177149827No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG11960.16049145812394072No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11930.16008888757680712No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC11780.15807603484113894No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11780.15807603484113894No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT11680.1567341330173602No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11560.15512385082882565No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC11540.15485547046406992No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG11460.15378194900504688No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC11430.15337937845791327No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11150.14962205335133272No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTG11120.14921948280419908No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTAT11080.14868272207468758No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG10750.14425444605621762No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCGTCGGTGTCA10610.14237578350292737No Hit
ACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAGACCACCCAAGGATCACCACTACTATCCCACACCTGACAGTA10510.1410338816791486No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10500.1408996914967707No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCGTCGGTGTCAGTGGC9900.1328482805540981No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGGCCGTGCTCCC9670.12976190635940693No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9650.1294935259946512No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT9630.12922514562989543No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAGCACGGCCACCCTGACCATCAGCAGGGTCGA9620.12909095544751753No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC9510.12761486344136092No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9490.12734648307660515No Hit
GTGCTGACTCAGCCACCGTCGGTGTCAGTGGCCCCAGGACAGACGGCCTG9340.125333630340937No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG9260.12426010888191398No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG9190.12332077760526886No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA9010.12090535432246706No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT8860.1188925015867989No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA8820.1183557408572874No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8790.11795317031015376No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT8780.11781898012777588No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC8760.11755059976302014No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA8700.11674545866875287No Hit
CTCCTGTACAGCCTCTGGATTCAGTTTCGGTGACTACTACATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG8690.11661126848637501No Hit
GATTACACTCTCACCATCAGCGGCCTGCAGCCTGAAGATTTTGCATCTTGTTACTGTCGACAGTATTATAGTACC8680.11647707830399713No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT8660.11620869793924136No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTT8610.11553774702735198No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG8590.11526936666259623No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA8560.1148667961154626No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC8430.11312232374455021No Hit
GTGTATTACTGTGCGAGAGATGGTCGTTTTACGAATTATTATGAGATTAG8340.1119146121031493No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT8280.11110947100888204No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCGTCGGTGTCAGTGGCCCCAGGACAGACGG8150.10936499863796965No Hit
GGATTACACTCTCACCATCAGCGGCCTGCAGCCTGAAGATTTTGCATCTTGTTACTGTCGACAGTATTATAGTAC8140.10923080845559177No Hit
TCTTTGATGATCGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAGCACGGCCACC8140.10923080845559177No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8000.1073521459023015No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG7770.10426577170761034No Hit
GGCCAGGCCCCTCTGCTGGTCGTCTTTGATGATCGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCC7720.10359482079572095No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7720.10359482079572095No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGGCCGTGCTCCCAGAGTTGGAGCCAG7670.10292386988383156No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA7630.10238710915432005No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGC7540.10117939751291916No Hit
GGACACAGCATGGACATGAGGGTGCCCGCTCAGCGCCTGGGGCTCCTGCT7530.10104520733054127No Hit
CTATTACTGTCAGGTGTGGGATAGTAGTGGTGATCCTTGGGTGGTCTTCGGCGGAGGGACCAGGCTGACCGTCCT7530.10104520733054127No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)