FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences745211
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTACCCCTCACCTTCGGCCAAGGGACACGGCTGGAGATTAAA37540.5037499446465498No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23890.32058034570074784No Hit
CCTATGTGCTGACTCAGCCACCGTCGGTGTCAGTGGCCCCAGGACAGACGGCCTGGATTTCGTGTGGGGGAAACA22510.30206210053260085No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20470.27468730332751395No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19510.2618050458192378No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAGACCACCCAAGGATCACCACTACTATCCCACACCTGACAGT19350.25965800290119173No Hit
CTATAATACTGTCGACAGTAACAAGATGCAAAATCTTCAGGCTGCAGGCCGCTGATGGTGAGAGTGTAATCCGTC18820.2525459232351643No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18810.2524117330527864No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18610.24972792940522884No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA17460.23429605843177304No Hit
CTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGGCCGTG16920.22704978858336766No Hit
CCCCAGGACAGACGGCCTGGATTTCGTGTGGGGGAAACAACATTGCAATTAAAAGTGTGCACTGGTACCAGCAGA16020.21497267216935875No Hit
GGATAGTAGTGGTGATCCTTGGGTGGTCTTCGGCGGAGGGACCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTGC15670.21027601578613309No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGAGTTCTTGGTGTTGTCTCTGG15490.20786059250333128No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT13590.18236445785153466No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT13340.17900970329208774No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTC13090.17565494873264081No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13050.1751181880031293No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCGTCGGTGTCAGTGGCCCCAGGAC12720.17068991198465938No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAGACCACCCAAGGATCACCACTACTATCCCAC12720.17068991198465938No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC12560.16854286906661337No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC12360.16585906541905582No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT12220.16398040286576554No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12200.16371202250100977No Hit
GGGTGGCCGTGCTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCGATCATCAA12150.1630410715891204No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG12010.16116240903583012No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCC12000.16102821885345225No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTG11840.1588811759354062No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC11730.15740508392924957No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11370.152574237363646No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10950.14693824970377517No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC10560.14170483259103797No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC10390.13942359949061406No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG10340.13875264857872469No Hit
AGTCAGGACACAGCATGGACATGAGGGTGCCCGCTCAGCGCCTGGGGCTCCTGCTGCTCTGGTTCCCAGTTTGC10280.13794750748445742No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCGTCGGTGTCA10200.1368739860254344No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTAT10130.13593465474878927No Hit
GTGCTGACTCAGCCACCGTCGGTGTCAGTGGCCCCAGGACAGACGGCCTG10000.13419018237787686No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT9990.134055992195499No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCGTCGGTGTCAGTGGC9930.13325085110123175No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA9790.13137218854794144No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9710.13029866708891844No Hit
CTCCTGTACAGCCTCTGGATTCAGTTTCGGTGACTACTACATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG9550.12815162417087242No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9400.12613877143520427No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9300.12479686961142548No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9240.12399172851715821No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9170.12305239724051309No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGC9130.12251563651100159No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG9070.12171049541673432No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG8970.12036859359295556No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC8920.11969764268106617No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG8920.11969764268106617No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA8890.11929507213393255No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8790.11795317031015376No Hit
ACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAGACCACCCAAGGATCACCACTACTATCCCACACCTGACAGTA8740.11728221939826437No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG8720.11701383903350863No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA8720.11701383903350863No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAGCACGGCCACCCTGACCATCAGCAGGGTCGA8600.11540355684497411No Hit
TCTTTGATGATCGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAGCACGGCCACC8590.11526936666259623No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC8380.11245137283266082No Hit
GTGTATTACTGTGCGAGAGATGGTCGTTTTACGAATTATTATGAGATTAG8280.11110947100888204No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG8280.11110947100888204No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT8270.11097528082650418No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGGCCGTGCTCCC8240.11057271027937053No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTT7980.10708376553754576No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT7980.10708376553754576No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA7920.10627862444327849No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7900.10601024407852273No Hit
GATTACACTCTCACCATCAGCGGCCTGCAGCCTGAAGATTTTGCATCTTGTTACTGTCGACAGTATTATAGTACC7860.10547348334901122No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG7810.10480253243712184No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCGTCGGTGTCAGTGGCCCCAGGACAGACGG7750.10399739134285457No Hit
GGATTACACTCTCACCATCAGCGGCCTGCAGCCTGAAGATTTTGCATCTTGTTACTGTCGACAGTATTATAGTAC7680.10305806006620943No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC7650.10265548951907581No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC7510.10077682696578553No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)