FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977467
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTATCCCACACCTGACAATAATAGTCGGCCTCATCACCGGCTTCGAC48820.49945420152291586No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCACCAGATGGTCACCACTACTATCCCACACCTGACAAT47210.48298305722853047No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG40160.41085786016305414No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37520.38384927573002464No Hit
GGATAGTAGTGGTGACCATCTGGTGGTTTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGC36380.37218647790667103No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT32960.33719808443661015No Hit
GGATTATCTGTGCGGGGAACAACATCGGAACTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC32620.33371970613841695No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA29520.3020050804784203No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC28890.2955598501023564No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCACCAGATGGTCACCACTACTATCCCAC28300.2895238407025506No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27850.2849201047196478No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC26140.2674259079846174No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC25380.259650709435715No Hit
CCCCAGGACAGACGGCCAGGATTATCTGTGCGGGGAACAACATCGGAACTAAAAGTGTGCACTGGTACCAGCAGA24610.251773205642748No Hit
CCCCTGTGTCGGTCGTCTATGATGATGGTGACCGGCCCTCAGGGATCCCT24190.2474763853920388No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG23500.24041732355158793No Hit
GGCTCTGTGACGTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTAT23400.23939427111094289No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT22850.23376748268739508No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC22640.23161907256204045No Hit
GAACTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTG22480.2299821886570084No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACGTCCTATGTGCTGACT22380.2289591362163633No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCACCAGATGGTCACCACTACTATCCCACACCTGACAATA22340.2285499152401053No Hit
CCTCTCTCACTGCACAGGCTCTGTGACGTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAC21460.2195470537624288No Hit
GTAATACACAGCCGTGTCTTCGGCCTTCAGACTGTTCATTTGTAGATACACTGAACTCTTGGCGTCGTCTCTGGA21370.21862630656584825No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG21140.21627328595236464No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTG21040.2152502335117196No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC20630.21105571850507485No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC20470.2094188346000428No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20430.20900961362378476No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACGTCCTATGTGCTGACTCAGCC20170.20634967727810757No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19890.20348513044430144No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19850.2030759094680434No Hit
ACACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTAGTT19790.2024620780036564No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGACCATCAACAGGGTCGA19720.20174594129520484No Hit
GTTGATGGTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCACC19640.20092749934268883No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC19410.19857447872920517No Hit
ATCCCACACCTGACAATAATAGTCGGCCTCATCACCGGCTTCGACCCTGTTGATGGTCAGGGTGGCCGTGTTCCC19400.19847217348514068No Hit
GTGTATTACTGTGCGAGAGGTCGTCCTTTCACATTTTTCCGTGAGACAAG19260.1970399000682376No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18830.19264077457346385No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTATCTGTGCGGGGAACA18110.18527479700081947No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACGTCCTATGTGCTGACTCAGC18110.18527479700081947No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG18080.18496788126862596No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17940.18353560785172288No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG17640.1804664505297877No Hit
GCACAGGCTCTGTGACGTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA17310.177090377475659No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC17230.176271935523143No Hit
GGCCAGGCCCCTGTGTCGGTCGTCTATGATGATGGTGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCC17080.1747373568621754No Hit
CTTTTAGTTCCGATGTTGTTCCCCGCACAGATAATCCTGGCCGTCTGTCCTGGGGCCACTGACACCGAGGGTGG17030.17422583064185287No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAG16950.17340738868933683No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCACCATCATCAT16810.17197511527243375No Hit
GACCATCAACAGGGTCGAAGCCGGTGATGAGGCCGACTATTATTGTCAGGTGTGGGATAGTAGTGGTGACCAT16770.17156589429617572No Hit
CGGCCACCCTGACCATCAACAGGGTCGAAGCCGGTGATGAGGCCGACTAT16640.17023592612333716No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16160.1653252744082409No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA16010.1637906957472733No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG15800.16164228562191868No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT15710.16072153842533815No Hit
TCTATGATGATGGTGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACC15680.16041462269314463No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACGTCCTA15540.15898234927624155No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC15510.15867543354404803No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA15240.1559131919543064No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTT15230.15581088671024187No Hit
CCTTGGTCCCTCCGCCGAAAACCACCAGATGGTCACCACTACTATCCCACACCTGACAATAATAGTCGGCCTCAT15200.15550397097804836No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC14550.1488541301138555No Hit
GAACAACATCGGAACTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCC14170.14496653083940428No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCACC14060.14384117315469472No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13980.1430227312021787No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13760.14077201583275958No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT13690.14005587912430803No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13510.13821438473114694No Hit
CTCACTGCACAGGCTCTGTGACGTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG13470.1378051637548889No Hit
GTGTGGGATAGTAGTGGTGACCATCTGGTGGTTTTCGGCGGAGGGACCAA13390.13698672180237287No Hit
GTGTCAGTGGCCCCAGGACAGACGGCCAGGATTATCTGTGCGGGGAACAA13180.13483831167701826No Hit
GTAGTGGTGACCATCTGGTGGTTTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCT13150.13453139594482472No Hit
CTATTATTGTCAGGTGTGGGATAGTAGTGGTGACCATCTGGTGGTTTTCGGCGGAGGGACCAAGGTGACCGTCCT12870.13166684911101859No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC12860.1315645438669541No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC12710.13002996520598648No Hit
GTGACGTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGG12610.12900691276534143No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCACCAGATGGT12350.1263469764196643No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC12330.12614236593153527No Hit
CAATAATAGTCGGCCTCATCACCGGCTTCGACCCTGTTGATGGTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCC12280.12563083971121275No Hit
CCTGAGGGCCGGTCACCATCATCATAGACGACCGACACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCAC12170.12450548202650319No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCC12120.12399395580618067No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGG11930.12205015616895507No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA11860.12133401946050353No Hit
CATCATAGACGACCGACACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAG11650.1191856093351489No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11450.1171395044538588No Hit
CCACTACTATCCCACACCTGACAATAATAGTCGGCCTCATCACCGGCTTC11400.11662797823353628No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGG11390.11652567298947178No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACGTC11330.11591184152508474No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACA11310.11570723103695572No Hit
CCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTATCTGTGCGGGGAACAACATCGGAACTAAAAGTGTG11240.1149910943285042No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC11160.11417265237598814No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11150.11407034713192363No Hit
CACCACTACTATCCCACACCTGACAATAATAGTCGGCCTCATCACCGGCTTCGACCCTGTTGATGGTCAGGGTGG11100.1135588209116011No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC11040.11294498944721407No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT11010.11263807371502056No Hit
AGCCAGGCCAGGCCCCTGTGTCGGTCGTCTATGATGATGGTGACCGGCCC10610.10854586395244034No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCACCAGATGGTCACCACTACTATCCC10510.10752281151179528No Hit
TAGTAGTGGTGACCATCTGGTGGTTTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC10220.10455595943392462No Hit
GGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC10090.10322599126108606No Hit
ATTATTGTCAGGTGTGGGATAGTAGTGGTGACCATCTGGTGGTTTTCGGC10060.10291907552889253No Hit
CAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACC10030.10261215979669902No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)