FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977467
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTATCCCACACCTGACAATAATAGTCGGCCTCATCACCGGCTTCGAC42840.43827566557234154No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCACCAGATGGTCACCACTACTATCCCACACCTGACAAT40210.41136938638337667No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG37870.38742995927228235No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC36420.37259569888292904No Hit
GGATAGTAGTGGTGACCATCTGGTGGTTTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGC33130.33893727358570674No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA30540.3124402153729998No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30420.31121255244422574No Hit
GGATTATCTGTGCGGGGAACAACATCGGAACTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC29730.30415349060377483No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC25510.26098067760855354No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACGTCCTATGTGCTGACT24900.2547400577206187No Hit
CCCCTGTGTCGGTCGTCTATGATGATGGTGACCGGCCCTCAGGGATCCCT24130.24686255392765177No Hit
CCCCAGGACAGACGGCCAGGATTATCTGTGCGGGGAACAACATCGGAACTAAAAGTGTGCACTGGTACCAGCAGA23760.24307725989726509No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG22930.23458592463991113No Hit
GGCTCTGTGACGTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTAT22600.23120985158578242No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC22560.23080063060952444No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22450.22967527292481485No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT22430.22947066243668582No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC22320.22834530475197629No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCACCAGATGGTCACCACTACTATCCCAC22250.22762916804352473No Hit
CCTCTCTCACTGCACAGGCTCTGTGACGTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAC21660.22159315864371892No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC20930.21412487582701No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTG20830.21310182338636496No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC20000.20461048812901098No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACGTCCTATGTGCTGACTCAGC19800.20256438324772089No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC19250.1969375948241731No Hit
GTGTATTACTGTGCGAGAGGTCGTCCTTTCACATTTTTCCGTGAGACAAG19230.19673298433604408No Hit
GAACTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTG19200.19642606860385056No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19000.19437996372256044No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG18490.18916239627527068No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18420.18844625956681912No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC18050.18466096553643244No Hit
ATCCCACACCTGACAATAATAGTCGGCCTCATCACCGGCTTCGACCCTGTTGATGGTCAGGGTGGCCGTGTTCCC17940.18353560785172288No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCACCAGATGGTCACCACTACTATCCCACACCTGACAATA17830.18241025016701332No Hit
GCACAGGCTCTGTGACGTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA17650.1805687557738522No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAG17610.1801595347975942No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACGTCCTATGTGCTGACTCAGCC17550.17954570333320716No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCACCATCATCAT17320.17719268271972352No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17230.176271935523143No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTATCTGTGCGGGGAACA17140.17535118832656243No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17080.1747373568621754No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT16920.17310047295714331No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16900.1728958624690143No Hit
GTAATACACAGCCGTGTCTTCGGCCTTCAGACTGTTCATTTGTAGATACACTGAACTCTTGGCGTCGTCTCTGGA16800.17187281002836924No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG16630.17013362087927264No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16350.16726907404546648No Hit
CTTTTAGTTCCGATGTTGTTCCCCGCACAGATAATCCTGGCCGTCTGTCCTGGGGCCACTGACACCGAGGGTGG16330.1670644635573375No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACGTCCTA16290.16665524258107944No Hit
GACCATCAACAGGGTCGAAGCCGGTGATGAGGCCGACTATTATTGTCAGGTGTGGGATAGTAGTGGTGACCAT16280.16655293733701496No Hit
CGGCCACCCTGACCATCAACAGGGTCGAAGCCGGTGATGAGGCCGACTAT16270.16645063209295047No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGACCATCAACAGGGTCGA16210.16583680062856343No Hit
ACACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTAGTT16180.16552988489636988No Hit
GTTGATGGTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCACC16130.16501835867604736No Hit
GGCCAGGCCCCTGTGTCGGTCGTCTATGATGATGGTGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCC16010.1637906957472733No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC15710.16072153842533815No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG15380.15734546537120947No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15240.1559131919543064No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC15120.15468552902553231No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA14790.15130945597140363No Hit
CCTTGGTCCCTCCGCCGAAAACCACCAGATGGTCACCACTACTATCCCACACCTGACAATAATAGTCGGCCTCAT14770.15110484548327463No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14660.14997948779856507No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA14620.14957026682230704No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14560.14895643535792No Hit
TCTATGATGATGGTGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACC14500.14834260389353296No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC14170.14496653083940428No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13930.14251120498185615No Hit
GTGTGGGATAGTAGTGGTGACCATCTGGTGGTTTTCGGCGGAGGGACCAA13880.14199967876153363No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCACC13720.14036279485650155No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTT13510.13821438473114694No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC13360.13667980607017935No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACGTC13180.13483831167701826No Hit
GAACAACATCGGAACTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCC13060.1336106487482442No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCACCAGATGGTCACCACTACTATCCC12900.1319737648432121No Hit
CTCACTGCACAGGCTCTGTGACGTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG12790.13084840715850254No Hit
GTAGTGGTGACCATCTGGTGGTTTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCT12540.1282907760568899No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCACCAGATGGT12460.12747233410437386No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC12450.12737002886030935No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12180.1246077872705677No Hit
GTGTCAGTGGCCCCAGGACAGACGGCCAGGATTATCTGTGCGGGGAACAA12120.12399395580618067No Hit
CTATTATTGTCAGGTGTGGGATAGTAGTGGTGACCATCTGGTGGTTTTCGGCGGAGGGACCAAGGTGACCGTCCT11640.1190833040910844No Hit
CCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTATCTGTGCGGGGAACAACATCGGAACTAAAAGTGTG11610.1187763883588909No Hit
GTGACGTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGG11550.11816255689450386No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA11470.1173441149419878No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGG11440.1170371992097943No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11180.11437726286411716No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT11080.11335421042347209No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT10950.11202424225063352No Hit
CAATAATAGTCGGCCTCATCACCGGCTTCGACCCTGTTGATGGTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCC10780.11028505310153693No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACA10720.1096712216371499No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC10660.10905739017276286No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC10550.1079320324880533No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC10530.1077274219999243No Hit
CCACTACTATCCCACACCTGACAATAATAGTCGGCCTCATCACCGGCTTC10350.1058859276067632No Hit
CACCACTACTATCCCACACCTGACAATAATAGTCGGCCTCATCACCGGCTTCGACCCTGTTGATGGTCAGGGTGG10260.10496518041018266No Hit
CATCATAGACGACCGACACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAG10190.1042490437017311No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGG10160.10394212796953758No Hit
CTGCACAGGCTCTGTGACGTCCTATGTGCTGACTCAGCCACCCTCGGTGT10060.10291907552889253No Hit
ATTATTGTCAGGTGTGGGATAGTAGTGGTGACCATCTGGTGGTTTTCGGC10050.10281677028482802No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC10020.10250985455263452No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGATCCACGTCAGCCAAAAAATTTCAAAGGGGAATCCAGAGGCTGCAC9870.10097527589166694No Hit
GGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGTCGGTCGTCTATGATGATGGTGACCGGCCCTCAGGGATCCCTG9850.10077066540353792No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)