FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences582640
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT24400.4187834683509543No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT21220.3642043114101332No Hit
GTATAATAGTTATTCTAACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT20450.3509886035974187No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19100.3278182067829191No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA19050.32696004393793765No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTGTGTC18100.3106549498832899No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16540.2838802691198682No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG14590.2504119181655911No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC14420.24749416449265416No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG14070.2414870245777839No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCA13540.2323904984209804No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC12830.2202045860222436No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC12720.21831662776328437No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12680.21763009748729917No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA12060.20698887820952902No Hit
CTGCTGATACCAGGCCAACCAGTTATTAATATTCTGACCGGCCCGGCAAGTGATGGTGACTCTGTCTCCTAC11820.202869696553618No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11380.19531786351778113No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGG11220.19257174241384045No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10810.18553480708499243No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10640.18261705341205547No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC10540.18090072772209256No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAG10300.17678154606618152No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG10110.17352052725525194No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG10070.17283399697926677No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT9770.167685019909378No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9760.1675133873403817No Hit
TCCCTGGTCTCTGCTGATACCAGGCCAACCAGTTATTAATATTCTGACCGGCCCGGCAAGTGATGGTGACT9530.163565838253467No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCC9490.16287930797748182No Hit
GTTATTAATATTCTGACCGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGG9180.15755869833859673No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9140.15687216806261156No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT9140.15687216806261156No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8710.14949196759577096No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC8670.1488054373197858No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8400.1441713579568859No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCGGTCAGA8290.1422833996979267No Hit
TTAATATTCTGACCGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA8210.14091033914595635No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8070.13850748318000825No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC8060.13833585061101195No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG7960.136619524921049No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG7960.136619524921049No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7950.13644789235205274No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCGGTCAGAATATTAATAACTGGTTGGCCTGGTATCAGCAG7800.13387340381710833No Hit
GATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGC7750.13301524097212686No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7690.13198544555814912No Hit
GAGTGAATTGTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCTAAAGTAGACGCCTTAT7690.13198544555814912No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG7650.13129891528216395No Hit
CCTTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTCTCTGCTGATACCAGGCCAACCAGTTATTAATATTCTGAC7640.13112728271316765No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7580.13009748729918988No Hit
GCTGATACCAGGCCAACCAGTTATTAATATTCTGACCGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATG7530.12923932445420844No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT7470.1282095290402307No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7380.12666483591926406No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAA7370.12649320335026776No Hit
CAGTATAATAGTTATTCTAACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT7360.12632157078127146No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT7360.12632157078127146No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7270.12477687766030482No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7240.12426197995331595No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT7200.12357544967733078No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA6980.11979953315941233No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC6880.1180832074694494No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC6830.11722504462446794No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC6800.11671014691747907No Hit
GACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCGTCTACTTTAGAA6580.11293423039956062No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGG6570.11276259783056433No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6510.11173280241658658No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT6390.10967321158863105No Hit
GGTATCAGCAGAGACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCGTCTACTTTAGAAAGTGGGGTCCCAT6380.10950157901963475No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATG6300.10812851846766441No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCGGTCAGAATATTAATA6300.10812851846766441No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG6240.10709872305368667No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTC6240.10709872305368667No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCT6180.1060689276397089No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT6160.10572566250171632No Hit
ACCTTGATGGGACCCCACTTTCTAAAGTAGACGCCTTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTCTCTG6100.10469586708773858No Hit
GCTTAGGGGCTTTCCCTGGTCTCTGCTGATACCAGGCCAACCAGTTATTA6100.10469586708773858No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT6090.10452423451874228No Hit
GGTCTCTGCTGATACCAGGCCAACCAGTTATTAATATTCTGACCGGCCCGGCAAGTGATGGTGACT6090.10452423451874228No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC6080.10435260194974598No Hit
CAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA6070.1041809693807497No Hit
GTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGC6070.1041809693807497No Hit
ACTTATTACTGCCAACAGTATAATAGTTATTCTAACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA6050.10383770424275711No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC5980.10263627625978307No Hit
TTCTAAAGTAGACGCCTTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTCTCTGCTGATACCAGGCCAACCAGTT5950.10212137855279417No Hit
GAATATTAATAACTGGTTGGCCTGGTATCAGCAGAGACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCGT5930.1017781134148016No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)