FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences885489
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG25620.2893316574231865No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGC24050.27160134117984525No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG21380.24144851037110568No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCG20110.22710615264560033No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCC18850.21287672687068954No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18500.20892410859988095No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT18020.20350337497134352No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC14780.16691342297871573No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13610.15370038475915568No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCTTGTATTTCCAGGAGAAAGTGATGG13270.14986069843894165No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGCCAGAGTGTTAGTC13230.14940897063656353No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCTGAACCTGCGGGAGTCGGCCACCATC13150.14850551503180728No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT12850.14511755651397137No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12450.14060027849019016No Hit
CTCCTGTGCAGCCTCTGGATTCACCTGTAGCAACCATGTCCTGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG12260.13845457142889409No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTCTCAAC11890.13427608925689646No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT11840.1337114295039238No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAGGGCAAAGATCATGTCAAGGTTGTTTGGGGTGGCTC11690.13201745024500586No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC11540.13032347098608793No Hit
GTATGTACTATCATCACTACCACTAATACCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCT11520.13009760708489884No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11450.12930708343073713No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC11280.12738724027063014No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG11020.12445100955517233No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC10830.12230530249387626No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC10800.12196650664209267No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT10740.1212889149385255No Hit
GGGTTCCCTGGCCCCAGAGGGCAAAGATCATGTCAAGGTTGTTTGGGGTGGCTCCTTTCGCACAGAAATATTCGG10700.12083718713614736No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCTGTGGCCCTGTT10660.12038545933376925No Hit
CTCCTGCAGGGCCAGCCAGAGTGTTAGTCACTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT10580.119482003729013No Hit
ACCATGTCCTGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTCTCAGGTATTAGTGGTAGTGATG10470.11823975227247317No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT10440.11790095642068958No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAG10370.11711043276652787No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCAGGACATGGTTGCTACAGGTGAATCCAGAGGCTGCAC10340.11677163691474429No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCACGGCG10280.11609404521117711No Hit
GTCCTGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTCTCAGGTATTAGTGGTAGTGATGATAGT10260.11586818130998804No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG10150.1146259298534482No Hit
TAGTACATACTACGCAGATTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACACTGTAT10120.11428713400166461No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA9850.1112379713356123No Hit
CCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAGCCAGAGCATGTTCACCTGCCGCGTGG9670.10920519622491076No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9520.1075112169659928No Hit
CCTGTGAGGTGGCTGCGTACTTGCCCCCTCTCAGGACTGATGGGAAGCCC9500.10728535306480373No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT9310.10513964600350767No Hit
GGTCTGTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCC9140.10321980284340064No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAACTGTTGGAGT9060.10231634723864441No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC8980.10141289163388817No Hit
GCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGGTTG8970.10129995968329365No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGCCAGAGTGTTAGTCACTACTTAGCCTGGT8950.10107409578210458No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)