FastQCFastQC Report
Wed 8 May 2024
EGAF00004717753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1019061
Total Bases75.1 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38010.37299042942473515No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT36020.3534626484577469No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24610.2414968289435078No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24490.2403192743123326No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG24200.23747351728699265No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23980.23531466712983815No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22690.22265595484470507No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21570.21166544495373682No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT20580.20195061924654167No Hit
GATCAGGAGCTCAGGAACTTTCCCTGGTTTCTGCTGATACCAGGCTAAAT19750.1938058663809134No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGGCATTAGCTT19410.19046946159258377No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC17820.17486686272951277No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17790.17457247407171894No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17760.17427808541392517No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17460.17133419883598724No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17390.17064729196780173No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT16660.16348383462815277No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA16660.16348383462815277No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGG15910.15612411818330799No Hit
CTCCTGATCCGTGCTGCATCCACTTTGCAATCAGGGGTCCCATCTCGGTT15900.15602598863071004No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15860.1556334704203183No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC15750.15455404534174108No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA15610.15318023160537003No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15600.15308210205277212No Hit
ACTTATTACTGTCAAAACTATCACGGAGCCCCGTTCACTTTCGGCGGAGG15490.15200267697419487No Hit
GTGCAGCCACAGTTCGTATGATCTCCACCTTGGTCCCTCCGCCGAAAGTG15200.14915691994885488No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCT14450.14179720350401007No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCT14110.13846079871568043No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG13970.13708698497930938No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13890.13630194855852593No Hit
GTAATAAGTTGCAACATCCTCAGGCTGCAGGCTGCTGATGGTGAGAGTGA13280.13031604585005216No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13210.12962913898186665No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT13140.12894223211368114No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTATGATCTCCACCTTGGTCC13060.12815719569289769No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAGGATGTTGCAACTTA12840.1259983455357432No Hit
AGTCAGGGCATTAGCTTTCATTTAGCCTGGTATCAGCAGAAACCAGGGAA12740.1250170500097639No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC12570.12334884761559907No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCC12030.1180498517753108No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA11560.11343776280320805No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG11490.11275085593502254No Hit
CACCATCAGCAGCCTGCAGCCTGAGGATGTTGCAACTTATTACTGTCAAA11240.11029761712007427No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGG11100.10892380338370324No Hit
TCCTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCC11060.10853128517331152No Hit
GCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACCAGATGTGACATCC10960.1075499896473322No Hit
GCCTGAGGATGTTGCAACTTATTACTGTCAAAACTATCACGGAGCCCCGT10900.10696121233174463No Hit
GTGCTGCATCCACTTTGCAATCAGGGGTCCCATCTCGGTTCAGTGGCAGT10710.10509675083238393No Hit
GGGCATTAGCTTTCATTTAGCCTGGTATCAGCAGAAACCAGGGAAAGTTC10710.10509675083238393No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10640.1044098439641984No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10550.10352667799081704No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10490.10293790067522944No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10260.10068092096547704No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)