FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1019061
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33160.32539759641473864No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT31460.3087155724730904No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23320.22883811665837472No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA22940.22510919365965337No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21360.2096047243491803No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG20060.19684788251144927No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT18770.1841891702263162No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17490.17162858749378104No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16820.16505390746971968No Hit
GATCAGGAGCTCAGGAACTTTCCCTGGTTTCTGCTGATACCAGGCTAAAT15930.15632037728850384No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG15890.1559278590781121No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG15740.15445591578914314No Hit
CTCCTGATCCGTGCTGCATCCACTTTGCAATCAGGGGTCCCATCTCGGTT15370.1508251223430197No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC15190.14905879039625697No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT14650.14375979455596868No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC14640.14366166500337077No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14350.14081590797803076No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGGCATTAGCTTTCATTTAGCCTGGTATCAGCAGAA13950.13689072587411352No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13740.13483000526955696No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13660.1340449688487735No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA13410.13159173003382527No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC13290.1304141754026501No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13270.13021791629745422No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCT12910.12668525240392872No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12440.12207316343182596No Hit
GTGCAGCCACAGTTCGTATGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAACGGGGCTCCGTGATAGTTTTGAC12370.12138625656364045No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACCAGATGTGACATCC12250.12020870193246529No Hit
GTAATAAGTTGCAACATCCTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCC12250.12020870193246529No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCC12230.12001244282726942No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT12040.11814798132790874No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11990.11765733356491907No Hit
ACTTATTACTGTCAAAACTATCACGGAGCCCCGTTCACTTTCGGCGGAGGGACCAAGGTGGAGATCATACGA11790.11569474251296047No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACCAGATGTGACAT11650.11432092877658943No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT11070.10862941472590945No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTATGATCTCCACCTTGGTCC10880.10676495322654875No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC10770.10568552814797152No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10720.10519488038498186No Hit
AGTCAGGGCATTAGCTTTCATTTAGCCTGGTATCAGCAGAAACCAGGGAAAGTTCCTGAGCTCCTGATCCGTG10590.10391919620120876No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAGGATGTTGCAACTTATTACTGTCAAAACTATCACGGAGCC10420.10225099380704393No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACC10400.10205473470184807No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC10250.10058279141287911No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)