FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772621
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32470.42025779780772204No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT30020.38854755436365307No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTACATTTGCAGATACA23910.30946609010109744No Hit
GATCTATGGTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTT23750.3073952170598521No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCC21560.27905014230780684No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21050.27244923448883734No Hit
GGCTTACAGTTTCCCTCCGCTCACCTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT20500.2653306084095566No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19870.25717654580965316No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG18690.24190385713046889No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG18590.24060956147969056No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGCATATTAGCAGCTGGTTAGCCTGGTATCAGCTGAA17980.2327143580099428No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17350.22456029541003936No Hit
CTGTAAGCCTGTTGACAAGAGTAAATTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTAAAATCTGTC16500.21355878237842357No Hit
CCATAGATCAGGAGCTTTGGGGCTTTCCCTGGTTTCAGCTGATACCAGGC15830.20488700151820882No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT15690.20307498760711915No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15010.19427377718182653No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG14740.19077917892472504No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA14610.18909659457871322No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC14300.18508427806130043No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCC14020.1814602502391211No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA13430.173823905899529No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC13140.17007044851227185No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12780.1654109841694699No Hit
GATTTTACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAATTTACTCTTGTCAACAGGCTTACAGTTTC12560.1625635337377576No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAGGTG12360.15997494243620092No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT12320.1594572241758896No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12290.1590689354806561No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12140.1571274920044886No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11500.14884399983950736No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC11410.14767913375380687No Hit
GCATATTAGCAGCTGGTTAGCCTGGTATCAGCTGAAACCAGGGAAAGCCCCAAAGCTCCTGATCTATGGTGCATC11210.1450905424522502No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAGGTGAGCGGAGGGAA10780.13952507115390342No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10590.13706590941742458No Hit
CTCCTGTGTAGCCTCTGGATTCAACTTTAGAAGTTATGGCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG10540.13641876159203542No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCC10440.1351244659412571No Hit
GTATATTACTGTGCGAAGGATTTAAGGGCAGTAAGTACTTACTACTTTGACTACTGGGGCCAGGGAACCCTGGTC10230.13240644507462263No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC10170.1316298676841556No Hit
GAGGGAAACTGTAAGCCTGTTGACAAGAGTAAATTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTAA10080.13046500159845512No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9920.1283941285572098No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT9920.1283941285572098No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9890.12800583986197633No Hit
GTTTCCCTCCGCTCACCTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA9860.1276175511667428No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG9750.12619382595088666No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9610.12438181203979701No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9540.12347580508425217No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT9390.1215343616080847No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9380.12140493204300686No Hit
GTCTCCATCTTCCGTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGCATATTAGC9370.12127550247792902No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAATTTACTCTTGTCAACAGGCTTACAGTTTCCCTCCGCTCAC9320.12062835465253985No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9250.11972234769699504No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC8860.11467459465895957No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT8840.11441573552880391No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8830.11428630596372606No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8700.11260372161771424No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8690.11247429205263641No Hit
GGTTAGCCTGGTATCAGCTGAAACCAGGGAAAGCCCCAAAGCTCCTGATC8380.1084619755352236No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC8330.10781482770983444No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8300.10742653901460095No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA8170.10574395466858913No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC8170.10574395466858913No Hit
CCTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG8160.1056145251035113No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8150.10548509553843345No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8140.10535566597335563No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8070.1044496590178108No Hit
GAGTAAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTGCAAATTGGATGCACCAT7970.10315536336703247No Hit
GCCTGGTATCAGCTGAAACCAGGGAAAGCCCCAAAGCTCCTGATCTATGGTGCATCCAATTTGCAAAGTGGGGTC7940.10276707467179898No Hit
ACCTTGATGGGACCCCACTTTGCAAATTGGATGCACCATAGATCAGGAGCTTTGGGGCTTTCCCTGGTTTC7890.10211992684640982No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTTACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTG7880.10199049728133197No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC7880.10199049728133197No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC7860.10173163815117632No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTTACTCTCACCATCAGCAGCCTGCAGCCTG7800.10095506076070933No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)