FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences924850
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32270.3489214467210899No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG28390.3069686976266422No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26270.28404606152349027No Hit
CTCCAGAACCATCCTTCGTACAATAATATACGGCCGTGTCTTCGGCTCTC24640.2664215818781424No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGC21580.23333513542736659No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT20280.21927880196788668No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCG19680.2127912634481267No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC19550.21138563010217873No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCC18560.2006811915445748No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG17690.19127426069092285No Hit
GTGTTAACACCTACTTAGCCTGGTTCCAGCAGAAACCTGGGCAGCCTCCCAGGCTCCTCATTTATGGTGCATCC15480.167378493809807No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGACTGCAGTCTGAAGATT15230.164675352759907No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14740.15937719630210304No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA14280.15440341677028707No Hit
CTCCTGTGCAGCCTCTGGATTCGCCTTTAGCAGTTATACCATGAACTGGGTCCGCCAGACTCCAGGGGAGGGG14080.1522409039303671No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13930.1506190193004271No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCTTGTATTTCCAGGAGAAAGTGATGG13750.14867275774449912No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13720.14834838081851112No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC13550.14651024490457912No Hit
GAGTTCACTCTCACCATCAGCAGACTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCATCATAATGCGTGG13000.14056333459479914No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCTGAACCTGCGGGAGTCGGCCACCATC12780.13818457047088717No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG12670.13699518840893118No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC12500.1351570524949992No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAGACTGCAGTCTG12320.1332107909390712No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT12270.1326701627290912No Hit
ATAATATACGGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGATACAGCGTATTCCTGGGATTGTCTCTGG12260.1325620370870952No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT12230.13223766016110722No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAACACCTACTTAGCCTGGTTCCAGCAGAAACCTGGGCAGCCTCCCAGGCT11620.12564199599935125No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT11610.12553387035735525No Hit
CTCCAGGGGAGGGGCTGGAGTGGGTCTCAACCATTGCTGATAGTGGTGTGAGCACACACTACGCGGACTCCGTGA11610.12553387035735525No Hit
GGGTTCCCTGGCCCCAGTACTCGTTCAGCACTCCAGAACCATCCTTCGTACAATAATATACGGCCGTGTCTTCGG11600.12542574471535925No Hit
GTGTGTGCTCACACCACTATCAGCAATGGTTGAGACCCACTCCAGCCCCTCCCCTGGAGTCTGGCGGACCCAGTT11460.12391198572741526No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAACAC11150.12056009082553928No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11030.11926258312158729No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC11010.11904633183759529No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG10700.1156944369357193No Hit
GTTCTGGAGTGCTGAACGAGTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGGAGTGCATCCGCCCCA10520.11374817537979133No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC10480.11331567281180732No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT10470.11320754716981132No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAACGTCCGAGGCCACGCATTATGATGCTGAC10420.11266691895983133No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC10270.11104503432989134No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAACACCTACTTAGCCTGGTT10220.11050440611991134No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTACTCGTTCAGCACTCCAGAACCATCCTTCGTACAATA10130.10953127534194734No Hit
GAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAAC9950.10758501378601934No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC9820.10617938044007137No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC9810.10607125479807536No Hit
CTCCAGAGACAATCCCAGGAATACGCTGTATCTGCAAATGAACAGCCTGA9790.10585500351408338No Hit
GAGCACACACTACGCGGACTCCGTGAAGGACCGCTTCACCATCTCCAGAGACAATCCCAGGAATACGCTGTAT9770.10563875223009137No Hit
CATTTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTC9740.10531437530410337No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC9730.10520624966210738No Hit
CTCCAGCCCCTCCCCTGGAGTCTGGCGGACCCAGTTCATGGTATAACTGCTAAAGGCGAATCCAGAGGCTGCAC9590.10369249067416339No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAG9550.10325998810617938No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT9550.10325998810617938No Hit
CTACGCGGACTCCGTGAAGGACCGCTTCACCATCTCCAGAGACAATCCCAGGAATACGCTGTATCTGCAAATGA9530.10304373682218737No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT9500.10271935989619937No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTG9490.10261123425420338No Hit
TTATGATGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGTCTGCTGATGGTGAGAGTGAACTCTGTCCC9430.10196248040222738No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC9320.1007730983402714No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9320.1007730983402714No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)