FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004717763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences630064
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT28500.4523350008887986No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26120.4145610604636989No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20460.32472891642753754No Hit
GATCTATGCTGCATCCAGTTTGCAAACTGGAGTCCCATCAAGGTTCAGTGGCGGTGGATCTGGGACAGATTTC19410.3080639427105818No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG18130.28774854617943574No Hit
GTAATACACAGCCGTGTCCTCGTCTCTCAAGCTGTTCAATTGCAAATGCAGTGAGTTCTCGGCGTTGTCTCTGG18110.2874311181086366No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17820.2828284110820488No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17620.27965413037405723No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG17130.2718771426394779No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT16990.2696551461438838No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15760.2501333197897357No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTGGCAGCTATTTAAATTGGTATCAGCAGAA15110.23981690748876303No Hit
GTGCAGCCACAGTTCGTTTGATTCCCACCTTGGTCCCTTGGCCGAACGTCCAGGGGATATTGAAACTGTGTTGAC14300.2269610706213972No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTGGC13820.21934279692221742No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT13780.2187079407806191No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC13470.2137878056832322No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13060.20728053023184945No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA12560.19934482846187054No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG12230.19410726529368444No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT12010.19061555651489373No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCC11950.18966327230249624No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA11840.1879174179131009No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11720.18601284948830593No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT11720.18601284948830593No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11540.18315599685111353No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACCGCC11310.17950557403692324No Hit
ACTTACTACTGTCAACACAGTTTCAATATCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGGAATCAAACGA11150.17696614947052997No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11020.17490286701033547No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGT10880.17268087051474135No Hit
GTTTCAATATCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGGAATCAAA10650.16903044770055106No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTCCCACCTTGGTCC10550.16744330734655527No Hit
ATGCTGCATCCAGTTTGCAAACTGGAGTCCCATCAAGGTTCAGTGGCGGTGGATCTGGGACAGATTTCACTCTC10530.16712587927575612No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC10530.16712587927575612No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10270.16299931435536708No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT10080.1599837476827751No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAACTGGAGTCCCATCAAGGTT9920.15744432311638185No Hit
AGTCAGAGCATTGGCAGCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATG9870.15665075293938394No Hit
GAGTGAAATCTGTCCCAGATCCACCGCCACTGAACCTTGATGGGACTCCAGTTTGCAAACTGGATGCAGCAT9630.15284161608979405No Hit
GTGTATTACTGTGCGAGGGGTAACCACTACGCTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTC9480.15046090555880037No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACACAGTTTCAATATC9460.15014347748800122No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9330.1480801950278067No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9290.1474453388862084No Hit
CCCCTGGACGTTCGGCCAAGGGACCAAGGTGGGAATCAAACGAACTGTGG9160.14538205642601387No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9140.14506462835521472No Hit
GGACCAAGGTGGGAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGA9100.14442977221361641No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCC9060.14379491607201808No Hit
CCTTGGTCCCTTGGCCGAACGTCCAGGGGATATTGAAACTGTGTTGACAG9060.14379491607201808No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC9000.1428426318596206No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA8990.14268391782422105No Hit
GTCCAGGGGATATTGAAACTGTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAG8790.13950963711622946No Hit
GTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCC8770.1391922090454303No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACACAGTTTCAATATCCCCTGGACGTT8750.13887478097463116No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC8660.13744635465603494No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8460.13427207394804336No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT8300.1317326493816501No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT8260.1310977932400518No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCC8250.13093907920465223No Hit
GTTCAGTGGCGGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTT8180.12982808095685516No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8120.1288757967444577No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCC8010.12712994235506234No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC7850.12459051778866909No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATT7790.12363823357627161No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACCGCCACTGAACCTTG7730.12268594936387416No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC7700.12220980725767541No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC7690.12205109322227584No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7580.12030523883288047No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT7580.12030523883288047No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACACAGTTTCAATATCCCCTGGACGTTCGGCCAAGGG7510.11919424058508342No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT7470.1185593844434851No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT7320.11617867391249143No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG7280.1155438177708931No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7270.11538510373549353No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT7260.11522638970009397No Hit
CAGTAATACACAGCCGTGTCCTCGTCTCTCAAGCTGTTCAATTGCAAATG7260.11522638970009397No Hit
CCACTGAACCTTGATGGGACTCCAGTTTGCAAACTGGATGCAGCATAGAT7230.11475024759389522No Hit
GTCCCAGATCCACCGCCACTGAACCTTGATGGGACTCCAGTTTGCAAACTGGATGCAGCATAGATCAGGAGCTT7110.11284567916910028No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG7110.11284567916910028No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7060.1120521089921024No Hit
ATGCAGCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGCTGCCAATGCT6940.11014754056730745No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATG6770.10744940196551461No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT6680.10602097564691841No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATC6620.10506869143452095No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC6620.10506869143452095No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTC6600.10475126336372179No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA6600.10475126336372179No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC6580.10443383529292263No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT6500.10316412300972601No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC6470.10268798090352727No Hit
ACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCAT6390.10141826862033064No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG6360.1009421265141319No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTC6350.10078341247873232No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG6310.10014855633713401No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTGCG406.484465E-495.4514970
AGGACTT650.00445123358.73938470
GTTTTGT700.00596840354.54371370
GTGGCCG5350.049.9559270
TCTCGCG3200.047.72574670
TATTGTT2950.047.45611270
CAGTTCG12050.044.35920370
TAATCCT406.027855E-541.778974
TCTTCGG2451.36442395E-841.55711470
CAGCCAT4050.040.8516770
GTGGTAT1100.039.5063861
AGAGACG2901.36788E-939.4971770
CTTATAC2450.038.203981
TATACAC2450.038.197913
TTATACA2550.036.6999552
TCGTATG2300.035.66610740
GTATCAA10500.035.6570471
AATCTCG2000.035.57716836
ATGTGAT2151.0946218E-535.51683470
ATCTCGT2050.034.77097737
Produced by FastQC (version 0.11.5)