FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences630064
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27500.43646359734884077No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24880.39488052007415114No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT20350.32298306203814214No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA18150.2880659742502349No Hit
GATCTATGCTGCATCCAGTTTGCAAACTGGAGTCCCATCAAGGTTCAGTGGCGGTGGATCTGGGACAGATTTC18000.2856852637192412No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17590.2791779882678585No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16920.2685441478960867No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT16620.2637827268340994No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16370.2598148759491099No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15510.24616546890474617No Hit
GTAATACACAGCCGTGTCCTCGTCTCTCAAGCTGTTCAATTGCAAATGCAGTGAGTTCTCGGCGTTGTCTCTGG15240.24188018994895757No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTGGC14180.22505650219660228No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTGGCAGCTATTTAAATTGGTATCAGCAGAA13630.21632723024962547No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG13600.21585108814342668No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG13510.2144226618248305No Hit
GTGCAGCCACAGTTCGTTTGATTCCCACCTTGGTCCCTTGGCCGAACGTCCAGGGGATATTGAAACTGTGTTGAC13510.2144226618248305No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC13480.21394651971863177No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT13180.20918509865664442No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC12490.19823383021407348No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGT12300.1952182635414815No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12110.1922026968688895No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12000.19045684247949415No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11680.18537799334670763No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCC11640.18474313720510932No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11570.18363213895731229No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10940.17363315472713883No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT10640.16887173366515149No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC10390.16490388278016202No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAACTGGAGTCCCATCAAGGTT10270.16299931435536708No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG10100.16030117575357425No Hit
ATGCTGCATCCAGTTTGCAAACTGGAGTCCCATCAAGGTTCAGTGGCGGTGGATCTGGGACAGATTTCACTCTC10050.15950760557657634No Hit
ACTTACTACTGTCAACACAGTTTCAATATCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGGAATCAAACGA9960.15807917925798015No Hit
GTTTCAATATCCCCTGGACGTTCGGCCAAGGGACCAAGGTGGGAATCAAA9890.1569681810101831No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACCGCC9810.15569846872698648No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC9760.1549048985499886No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT9760.1549048985499886No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9720.15427004240839026No Hit
GTGTATTACTGTGCGAGGGGTAACCACTACGCTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTC9680.15363518626679196No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTCCCACCTTGGTCC9630.15284161608979405No Hit
GAGTGAAATCTGTCCCAGATCCACCGCCACTGAACCTTGATGGGACTCCAGTTTGCAAACTGGATGCAGCAT9340.14823890906320628No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACACAGTTTCAATATC9290.1474453388862084No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA9270.1471279108154092No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT9100.14442977221361641No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8910.1414142055410244No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA8850.14046192132862692No Hit
GTCCAGGGGATATTGAAACTGTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAG8730.13855735290383198No Hit
AGTCAGAGCATTGGCAGCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATG8720.1383986388684324No Hit
CCTTGGTCCCTTGGCCGAACGTCCAGGGGATATTGAAACTGTGTTGACAG8520.13522435816044084No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC8350.13252621955864802No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC8290.13157393534625053No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8260.1310977932400518No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACACAGTTTCAATATCCCCTGGACGTT8120.1288757967444577No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCC7770.12332080550547246No Hit
GTTCAGTGGCGGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTT7760.12316209147007288No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCC7680.12189237918687626No Hit
CCCCTGGACGTTCGGCCAAGGGACCAAGGTGGGAATCAAACGAACTGTGG7670.12173366515147667No Hit
GGACCAAGGTGGGAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGA7600.12062266690367963No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACACAGTTTCAATATCCCCTGGACGTTCGGCCAAGGG7530.11951166865588259No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT7410.11760710023108764No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT7390.11728967216028847No Hit
GTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCC7280.1155438177708931No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7260.11522638970009397No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC7240.11490896162929479No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACCGCCACTGAACCT7130.11316310723989945No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACCGCCACTGAACCTTG7100.1126869651337007No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7070.11221082302750196No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC7050.11189339495670282No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG7030.11157596688590365No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCC6980.11078239670890577No Hit
GTCCCAGATCCACCGCCACTGAACCTTGATGGGACTCCAGTTTGCAAACTGGATGCAGCATAGATCAGGAGCTT6860.10887782828411083No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC6810.10808425810711292No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATT6790.10776683003631378No Hit
CCACTGAACCTTGATGGGACTCCAGTTTGCAAACTGGATGCAGCATAGAT6780.1076081160009142No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT6620.10506869143452095No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC6610.10490997739912135No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT6490.10300540897432642No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6480.10284669493892684No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC6460.10252926686812769No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6420.10189441072652936No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC6410.10173569669112978No Hit
ATGCAGCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGCTGCCAATGCT6320.10030727037253359No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATG6310.10014855633713401No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)