FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences798617
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34450.43137073215320987No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT30010.3757746203749733No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAAAC28220.353360872608522No Hit
GTATATTACTGTCAGCAGTATGGTAGGTCACTCACGTGGACCTTCGGCCAAGGGACCAAGGTGGCAATCAAGCGA23960.3000186572537274No Hit
ATATTACTGTCAGCAGTATGGTAGGTCACTCACGTGGACCTTCGGCCAAGGGACCAAGGTGGCAATCAAGCGA22650.2836152999497882No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22190.27785534242321414No Hit
ATGGTAGGTCACTCACGTGGACCTTCGGCCAAGGGACCAAGGTGGCAATCAAGCGAACTGTGGCTGCACCAT21270.266335427370066No Hit
GTAATATACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC20710.2593233051638019No Hit
CTCCTGCAGGGCCAGTCAAATTGTTAGCAGCAGCTACTTAGCCTGGTACC19660.24617557602705678No Hit
GTATGGTAGGTCACTCACGTGGACCTTCGGCCAAGGGACCAAGGTGGCAATCAAGCGAACTGTGGCTGCACCAT19590.24529906075127375No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG19400.24291994785986273No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18630.23327827982624963No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18600.2329026304223426No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG18230.22826962110748958No Hit
CAGTAATATACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC17470.21875316954184545No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT17020.21311842848324042No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAAATTGTTAGC16860.21111496499573637No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAAATTGTTAGCAGC15740.19709072058320823No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT15640.19583855590351823No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT15580.1950872570957042No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15450.19345944301210719No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA15110.18920208310116116No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG14790.1851951561261531No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14240.178308250387858No Hit
GTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAAA13500.16904223175815192No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAA13490.1689170152901829No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA12520.15677101789718975No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC12500.15652058496125176No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA12120.15176235917842973No Hit
ACCTACCATACTGCTGACAGTAATATACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGT12050.1508858439026467No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC11980.1500093286268637No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11930.14938324628701868No Hit
GTGCAGCCACAGTTCGCTTGATTGCCACCTTGGTCCCTTGGCCGAAGGTCCACGTGAGTGACCTACCATACTG11910.1491328133510807No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTATATTACTGTCAGCAGTATGGTAGGTC11640.14575196871591764No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11610.14537631931201064No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATTGCCACCTTGGTCCCTTGGCCGAAGGTCCACGTGAGTG11560.14475023697216563No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC11220.14049287706121957No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT10780.13498335247058352No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGTACCAT10760.1347329195346455No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10630.1331051054510485No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT10130.12684428205259843No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10060.12596776677681543No Hit
GTCCACGTGAGTGACCTACCATACTGCTGACAGTAATATACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTG9690.12133475746196236No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9590.12008259278227236No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT9550.11958172691039635No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9550.11958172691039635No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9540.11945651044242736No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9500.11895564457055133No Hit
CATCTATGGTACATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTC9490.11883042810258233No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT9320.11670174814710932No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9320.11670174814710932No Hit
CTACCATACTGCTGACAGTAATATACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC9250.1158252328713263No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC9230.1155747999353883No Hit
CACGTGGACCTTCGGCCAAGGGACCAAGGTGGCAATCAAGCGAACTGTGG9230.1155747999353883No Hit
ATGGTACATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTC9230.1155747999353883No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAAACACCGGAGAACTTGTGTTGACGCAGTCTC9190.1150739340635123No Hit
CTGCTACTCTGGCTCCCAGAAAACACCGGAGAACTTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCC9070.11357133644788427No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC9070.11357133644788427No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8410.10530704956193018No Hit
GTCAAATTGTTAGCAGCAGCTACTTAGCCTGGTACCAGCAAAAACCTGGC8320.10418010135020918No Hit
ATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATC8300.10392966841427118No Hit
GGACCAAGGTGGCAATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGA8290.10380445194630218No Hit
ACTTAGCCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAGGCTCCTCATC8130.10180098845879815No Hit
GCCTGGGAGCCTGGCCAGGTTTTTGCTGGTACCAGGCTAAGTAGCTGCTGCTAACAATTTGACTGGCCCTGCAGG7990.10004795790723212No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)