FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004717767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83
Sequences flagged as poor quality0
Sequence length56-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAATATGCTAACTTTCTGAATCAAAATGGAGAGCCTCTCAAGAAA11.2048192771084338No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATATCCAACTTGGTCCCCTGGCCAAAACTGTACGGATAAGA11.2048192771084338No Hit
AACCATAATTCGCCCCTCTCGCACAGTAATACACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATCTGCAGATAC11.2048192771084338No Hit
GTTATGTACTGTTTGAATTAATAAATTAAAGCTAAATAGGCTCTTCTCGTCATTATTTTTTATTCCCTAATAATC11.2048192771084338No Hit
GTACGGGGTGAGACCAAAATGGTGGAAACAGCCTTAACCCCAGATGCCTGCTATCCTGACTAATTTAAGTCATTG11.2048192771084338No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11.2048192771084338No Hit
GGTGAAGGTGGCACCAGAGGCATCTCTCAGGCCGGTCAGTGTGCACGTGA11.2048192771084338No Hit
GATTGTTCCTCTGCACAACAGGGAGAATATCTCTGATCCCACCTCACCAT11.2048192771084338No Hit
CTCCTGTGCAGCCTCTGGATTCGACTTCAGTTCCACTGCCGTGCACTGGG11.2048192771084338No Hit
GACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCG11.2048192771084338No Hit
AAAAGACTTTGGCCTCACTCCACTAGCAATACACCATACACAAAACATAACATATGCCACAAATAATAAACAAGT11.2048192771084338No Hit
GAATTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT11.2048192771084338No Hit
CCCTCCGCGTCCGGGTCTCCTGGACAATCAGTCGCCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTAT11.2048192771084338No Hit
GACTCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCATCATAGACGACCAGCACAGGGGCCTGGCCTGGGTT11.2048192771084338No Hit
GCCACAGAGGTCACGCTCAGGTGGTCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC11.2048192771084338No Hit
GTATCAACGCAGAGTACGGGAGCAGCCCCAGCGGTGGAGACGTCACCGGCTGCGCCCTTCAGTATCGCGGACGGA11.2048192771084338No Hit
CTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGG11.2048192771084338No Hit
GATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGTGGATGC11.2048192771084338No Hit
CTGTAATAGATGTACCCAATCCACTCCAGGCCCTTCCCTGGGAGCTGGCGGATCCAGTTCCAGTAATAACCCCC11.2048192771084338No Hit
CCAATGCACTTTCCAAAGATACTATTCACAAGCTGACTACTAAATGTGCCCCCTCGCTGCTGCGCAAACGGCAC11.2048192771084338No Hit
GTATTACTGTGCGAGAGTCCATAGCAGTGGCTGGTACGATCTTCTTGACTACTGGGGCCAGGGAACCCTGGTCAC11.2048192771084338No Hit
CACCCCCCACCACGGGAGACTAGAGCTGCAGGATCCCAGGGGAGGGGTCTCTCCTCCCACCCCAAGGCATCA11.2048192771084338No Hit
TCTCGCGGGGCAGCTCCTGTGACCCCTGCAGCCAGCGAACCAGCACGTCCTTGGGGCTGCAGCCGCGTGCCAGG11.2048192771084338No Hit
CGACCGCTTGGCGGGTAAACCCACCCATGTCAATGTGTCTGTTGTCATGGCGGAGGTGGACGGCACCTGCTACTG11.2048192771084338No Hit
GTCACGGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG11.2048192771084338No Hit
TTCCAGAAGTACCTTTTTGGCAGAATCATCCAAAGAGTTTTTCACAGCTGATCCATCCCACTTGTCAATTTTTAC11.2048192771084338No Hit
AAATCAAAATATACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAATAAAAAATAAAAAAAAAATTAAA11.2048192771084338No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCCTGGTCAAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC11.2048192771084338No Hit
CAAGAAGATGAAAGGATTGCTCTTGTTGACAACAAATGTAAGTGTGCCCG11.2048192771084338No Hit
GTTATAAAACAGAATACACAGTTGTCTGCATAGATCATAAAAAACATAAA11.2048192771084338No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA11.2048192771084338No Hit
CATGGGCCACCACGCAGGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGA11.2048192771084338No Hit
CTAATGCTCTGACTGGCCCGGCAAGTGATGGTGACCCTGTCACCCACAGA11.2048192771084338No Hit
GCACAGGAGAGTCTCAGGGACCCCCCAGGCTGGACCAAGCCTCCCCCAGACTCCACCAGCTGCACCTCAC11.2048192771084338No Hit
ACTACGTGCGTGCGGTGGGCTGGGCTCAGAAGTTAGCGCGAGCGAACCGCGAAGTGATGATGCAGCGAGTGGTC11.2048192771084338No Hit
CTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGTCAGAGAAGCGATTAGAAACCC11.2048192771084338No Hit
GTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC11.2048192771084338No Hit
TCTCTGCCAAATAATACAAAAATTATCCAGGTGTGGTGGCATGACACTGT11.2048192771084338No Hit
ATCAGGAGGTGTAATAAAATGTTCCTCGCATTCCCCCCCGCTTTTTTTTTTTTTTTTTGGGTTTGCCGTGGGGGG11.2048192771084338No Hit
CCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT11.2048192771084338No Hit
GAGTAGAGGCGAAGGGCAGGAAGGGTGTCGGGGGGCTCTGAAAGTGGGCA11.2048192771084338No Hit
GTTCTCCCTGATGCTGAAGTCTGTGAGTGCCGCGGACACGGCCGTGTATTAATGTGCGAGAGGCCACCCCCTACG11.2048192771084338No Hit
GAATAGTAAAGAAAATGCGGAAGCTCTGGCTCTCCAAGGCAAAGTCAAAA11.2048192771084338No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAAC11.2048192771084338No Hit
GTGCGAGGCAGCCAACGGCCACGCTGCTCGTATCCGACGGGGAATTCTCA11.2048192771084338No Hit
GTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCAC11.2048192771084338No Hit
GCCTGAGCCCCAGTGATGGTCAAGGAAGCTGTGTTTCCTGAGCTGGAGCC11.2048192771084338No Hit
GTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTT11.2048192771084338No Hit
CAACACTCAAAAAACCACAATAAATATAATAATAGACAACCGCAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAC11.2048192771084338No Hit
CTCTGTGACACTCTCCTGGGAGTTACACGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGTCTCTCTGGG11.2048192771084338No Hit
ATATGAGGAGTCTGGGAGCCTGGCCAGGTCTCTGCTGGTACCAGGCTTAGTAGTCGCTGCCAACACTGTGACTG11.2048192771084338No Hit
GTTCATAGGTTCCCAACTCTAACCCCACCCACGGGAGCCTGGAGCTGCAGGATCCCAGGGGAGGGGTCTCTCTCC11.2048192771084338No Hit
TATCAACGCAGAGTACGGGGAGGGGACAGGCTGCAGCCGGTGCAGTTACA11.2048192771084338No Hit
TATTTGAAGAACTGATTAATGTTTGGGTCTGAGTTTATATATCACAGTGAGAATTCTATGATGGACCATGTAACG11.2048192771084338No Hit
CATCTCCGAGCCCACGAGACGGCCTCCTATCTCGTATGCCGTCTTCTGCT11.2048192771084338TruSeq Adapter, Index 27 (96% over 26bp)
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAGC11.2048192771084338No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT11.2048192771084338No Hit
CTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGAC11.2048192771084338No Hit
GGAGAGGCCCCCATCAGGGACCAGACTGTTCCAAAAAAAAAACAAAAAACAAAAAC11.2048192771084338No Hit
ATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGTAGCAGGTCTCTGTAGCACTGTCTTC11.2048192771084338No Hit
CGCCTCGCCCTTACGCAGCTGCAGGTTGGCGGTGTCGATGGCCGGGCGTGGTGCCTGCGGTGCCAGCTCGACGG11.2048192771084338No Hit
GGGCTTAAAGGAAGTTTTACTGTGACCAAAGTTCTGTGATTGAGGGGGTGTGGAGAAAACAAAAAGCCTGGCCA11.2048192771084338No Hit
GTCACAAAGAGCTTCAACAGGGGAGAGAGTTAGAGGGAGAAGTGCCCCGACCGCCACCGCAGTTGCAGCCGG11.2048192771084338No Hit
GTCAGGCTCAGGTAGCTGCTGGCCCCGTACTTGTTGTTCCTCTTTTTGGA11.2048192771084338No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT11.2048192771084338No Hit
TCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATT11.2048192771084338No Hit
ATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGCGGCACTACTCCCTCTAACACTCTACCCTCTTGAAGC11.2048192771084338No Hit
TCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGCTGACCCAGTCTC11.2048192771084338No Hit
GTCAAAGATGTCACGGCTGCGCTGGGAGAAGGTGGAGCTCATGCCTCTCA11.2048192771084338No Hit
CCTGAGTCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGT11.2048192771084338No Hit
CACATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTG11.2048192771084338No Hit
TCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGAT11.2048192771084338No Hit
AAGGTGCTGATCGCCTACGGCTCCGATGCCGAATCGGGCATGTGGTCGAAGGCCATGCGCCTGCGCGGCCAGGTG11.2048192771084338No Hit
CCTTCCACCCTGTCTGCATCTGTGGGTGACAGGGTCACCATCACTTGCCG11.2048192771084338No Hit
GACAGCTGTAGCACTGTCTTCATAACAGAAATTGCTATGGGTAGCAGTAACTATCTGATTATACAGCTCAACTT11.2048192771084338No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGA11.2048192771084338No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG11.2048192771084338No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAATTTTTGATTTTTTTT11.2048192771084338No Hit
GTATCAACGCAGAGTACGGGATACTTTCTGAGAGTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTT11.2048192771084338No Hit
CCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCC11.2048192771084338No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT11.2048192771084338RNA PCR Primer, Index 12 (95% over 24bp)
GTCGATAACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAAC11.2048192771084338No Hit
GAATTATTGTTCCTCTGAACAACAGGGAGAATATCTCTGATCCCACCTCACCATTGAGAACCAGATTTGTGTACC11.2048192771084338No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)