FastQCFastQC Report
Wed 8 May 2024
EGAF00004717771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549920
Total Bases40.7 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCT36570.6650058190282223No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32000.5819028222286877No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT31820.5786296188536514No Hit
GTATAATAGTTATTCTCCGACGACGTTCGGCCAAGGGACCAAGGTGGAAA31000.5637183590340413No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22530.40969595577538553No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC22190.4035132382892057No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21950.39914896712249054No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG20090.36532586558044805No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19800.3600523712540006No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT18050.3282295606633692No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGATTATTAGTAG17740.3225923770730288No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17330.31513674716322376No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG17200.3127727669479197No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA17000.3091358743089904No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC16900.3073174279895257No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16230.2951338376491126No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA15930.28967849869071866No Hit
GATTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGACAGCCC15890.2889511201629328No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15320.27858597614198427No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA15310.27840413151003784No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15080.27422170497526915No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA14870.27040296770439337No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14810.26931189991271454No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA14810.26931189991271454No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14680.2669479196974105No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCT13960.25385510619726503No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC13730.24967267966249637No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13330.24239889438463774No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12920.2349432644748327No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC12810.2329429735234216No Hit
GCCCCTAAGCTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCC11960.21748617980797205No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC11720.2131219086412569No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC11710.21294006400931045No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG11370.20675734652313063No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11370.20675734652313063No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG11280.20512074483561246No Hit
CCTTATAGATCAGGAGCTTAGGGGCTGTCCCTGGTTTCTGCTGATACCAG11130.20239307535641549No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC11000.20002909514111145No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT10870.1976651149258074No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCC10710.19475560081466395No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10690.19439191155077104No Hit
GCTTAGGGGCTGTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTA10500.1909368635437882No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT10140.18439045679371546No Hit
GATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCG10090.18348123363398314No Hit
GTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCC9800.17820773930753564No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9720.17675298225196392No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9720.17675298225196392No Hit
TCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGC9690.17620744835612454No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT9430.17147948792551643No Hit
GGTATCAGCAGAAACCAGGGACAGCCCCTAAGCTCCTGATCTATAAGGCG9270.168569973814373No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA9190.16711521675880128No Hit
ACCTTGATGGGACCCCACTTTCTAAACTAGACGCCTTATAGATCAGGAGC9180.16693337212685483No Hit
GTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTAATAATCTGACT9010.16384201338376492No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8920.16220541169624672No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8910.16202356706430027No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAAC8890.16165987780040733No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC8770.15947774221704974No Hit
CAGTATAATAGTTATTCTCCGACGACGTTCGGCCAAGGGACCAAGGTGGA8720.15856851905731742No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8680.15784114052953155No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8590.15620453884201338No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG8570.15584084957812044No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8390.15256764620308408No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCC8380.15238580157113762No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8280.15056735525167297No Hit
CTAATAATCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA8260.15020366598778004No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8220.1494762874599942No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC8180.14874890893220832No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8010.1456575501891184No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7940.14438463776549315No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG7930.1442027931335467No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC7910.14383910386965376No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT7840.14256619144602853No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC7820.1422025021821356No Hit
TTATAGATCAGGAGCTTAGGGGCTGTCCCTGGTTTCTGCTGATACCAGGC7740.14074774512656388No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC7710.14020221123072446No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7690.13983852196683155No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATC7660.13929298807099214No Hit
ACTTATTACTGCCAACAGTATAATAGTTATTCTCCGACGACGTTCGGCCA7660.13929298807099214No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC7660.13929298807099214No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7630.13874745417515275No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7600.13820192027931336No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC7510.13656531859179516No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGA7460.13565609543206286No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA7450.13547425080011638No Hit
ATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGACAGCCCCTAA7360.13383764911259818No Hit
ACCAGGGACAGCCCCTAAGCTCCTGATCTATAAGGCGTCTAGTTTAGAAA7360.13383764911259818No Hit
AGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGAC7270.13220104742508001No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC7260.13201920279313353No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7020.1276549316264184No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT7010.12747308699447193No Hit
TTCTAAACTAGACGCCTTATAGATCAGGAGCTTAGGGGCTGTCCCTGGTT6950.12638201920279313No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT6940.12620017457084667No Hit
AACCAGGGACAGCCCCTAAGCTCCTGATCTATAAGGCGTCTAGTTTAGAA6820.12401803898748909No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGA6790.1234725050916497No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT6750.12274512656386383No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC6660.12110852487634564No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA6650.12092668024439918No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA6610.12019930171661333No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC6580.11965376782077392No Hit
ACCCAGTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCAT6550.11910823392493454No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC6530.1187445446610416No Hit
GTGTATTACTGTGCGAGAGGTCACCGATATTGTAGTAGCACCAGCTGCTA6460.11747163223741636No Hit
CCTTGGTCCCTTGGCCGAACGTCGTCGGAGAATAACTATTATACTGTTGG6310.11474396275821938No Hit
GCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTT6300.11456211812627291No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT6290.11438027349432645No Hit
GGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC6290.11438027349432645No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6270.11401658423043352No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCT6210.11292551643875472No Hit
GAGTCACCATCACTTGCCGGGCCAGTCAGATTATTAGTAGCTGGTTGGCC6170.11219813791096887No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGATTATTAGT6080.11056153622345068No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG6040.10983415769566483No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA5970.10856124527203959No Hit
CAGTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGA5900.10728833284841431No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA5890.10710648821646786No Hit
GCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCGTCGG5880.10692464358452139No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG5870.10674279895257492No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGT5850.10637910968868199No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC5820.10583357579284261No Hit
CTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGG5780.10510619726505674No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG5760.1047425080011638No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA5720.10401512947337795No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG5700.10365144020948501No Hit
CTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCT5670.10310590631364563No Hit
GAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGACATGAGGGTCCC5650.1027422170497527No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT5610.10201483852196683No Hit
GTACGGGAGTCAGACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCG5580.10146930462612744No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT5560.1011056153622345No Hit
ATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATT5560.1011056153622345No Hit
GATCAGGAGCTTAGGGGCTGTCCCTGGTTTCTGCTGATACCAGGCCAACC5550.10092377073028803No Hit
GTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACA5550.10092377073028803No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)