FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1201143
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTGTACTGGTCGTCCATGATGATGGCGACCGGCCCTCAGGGATCCCT81170.6757729928909381No Hit
GTACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGTACACTTTTAATT74340.6189104877604082No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC52480.4369171697291663No Hit
CCTATGTGGTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAAGATTACTTGTGTCGGAAACG51880.4319219276972017No Hit
ACCTAGGACGGTGGCCTTGGTCCCAGCTCCGAAGACATAATGATCACTACTACTATCCCAGACCTGACAGTAAT47880.3986203141507714No Hit
GACCTAGGACGGTGGCCTTGGTCCCAGCTCCGAAGACATAATGATCACTACTACTATCCCAGACCTGACAGTAAT47750.3975380117105124No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT45230.3765579951762613No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGGCCTTGGTCCC45030.37489291449893974No Hit
GTAATATACGGCCGTGTCGTCGGCTCTCAGGCTGGTCATTTGCAGAGACAGCGTCTTCTTGGAAAGGTCTCTGG42110.3505827366100456No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG40380.33617978875121446No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC38990.32460747804382994No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35350.2943030097165783No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC34830.28997379995554234No Hit
CCCCAGGACAGACGGCCAAGATTACTTGTGTCGGAAACGACATTGGAATTAAAAGTGTACACTGGTACCAGCAGA33090.2754875980628451No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC32940.27423878755485404No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTC29870.2486797991579687No Hit
GCCTCTGTGACCTCCTATGTGGTGACTCAGCCACCCTCGGTGTCAGTGGC29460.24526638376945958No Hit
CCTCTCTCACTGCACAGCCTCTGTGACCTCCTATGTGGTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAC29250.24351804905827198No Hit
CTACTACTATCCCAGACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTC28840.2401046336697629No Hit
GTACTGGTCGTCCATGATGATGGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAAC28200.23477637550233404No Hit
GCCTTGGGCTGACCTAGGACGGTGGCCTTGGTCCCAGCTCCGAAGACATAATGATCACTACTACTATCCCAGAC27110.22570168581093178No Hit
GTATATTACTGTGCGAAAGGAGGCGGATTGATAGGGGATGAAACTAGTGG27030.22503565354000313No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGCCTCTGTGACCTCCTATGTGGTGACT26210.21820882276298495No Hit
TAGTAGTAGTGATCATTATGTCTTCGGAGCTGGGACCAAGGCCACCGTCCTAGGTCAGCCCAAGGCCAACCCC25870.21537818561153835No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG25560.21279731056169No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGGCCTTGGTCCCAGCTCCGAAGACATAATGATC24760.20613698785240392No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGT24540.20430539910735024No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG24240.20180777809136796No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGCCTCTGTG24210.20155801598976975No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGCCTCTGTGACCTCCTATGTGGTGACTCAGCC24210.20155801598976975No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTC24050.20022595144791253No Hit
CTCACTGCACAGCCTCTGTGACCTCCTATGTGGTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG23900.19897714093992136No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT22790.18973594318078696No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCCATCATCATGG22390.18640578182614395No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGACCATCAGCAGGGTCGA22360.1861560197245457No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22150.1844076850133581No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGG21320.17749760020247382No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGGTCTGGGATAGTAGT20990.1747502170848933No Hit
GTAGTAGTGATCATTATGTCTTCGGAGCTGGGACCAAGGCCACCGTCCTAGGTCAGCCCAAGGCCAACCCC20300.16900568874813407No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGCCTCTGTGACCTCCTATGTGGTGACTCAGC19840.16517600319029457No Hit
CTACTATCCCAGACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGAC19340.16101330149699078No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGCCTCTGTGACCTCCTA19220.16001425309059789No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG19140.15934822081966926No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCCATTTTAAAAGGTGTCC18910.15743337804074953No Hit
GATTACTTGTGTCGGAAACGACATTGGAATTAAAAGTGTACACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGT18900.15735012400688345No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT18690.15560178929569585No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC18650.15526877316023155No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGG18600.15485250299090117No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTG18500.1540199626522404No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGG18210.1516055956701242No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG17990.14977400692507054No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT17740.14769265607841864No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTG17700.14735963994295434No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCCAG17470.14544479716403458No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGA17340.1443624947237756No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC16950.14111558740299865No Hit
CCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAAGATTACTTGTGTCGGAAACGACATTGGAATTAAAAGTGT16670.1387844744547485No Hit
GTGTACACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTACTGGTCGTC16550.1377854260483556No Hit
GACCAGTACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGTACACTTTTAATTCCAATGTCGTTTCCGACACA16430.1367863776419627No Hit
TCCATGATGATGGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACC16390.1364533615064984No Hit
CACTACTACTATCCCAGACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG15840.13187438964386422No Hit
CTATTACTGTCAGGTCTGGGATAGTAGTAGTGATCATTATGTCTTCGGAG15710.13079208720360522No Hit
GGCCAGGCCCCTGTACTGGTCGTCCATGATGATGGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCC15370.12796145005215864No Hit
CTGCACAGCCTCTGTGACCTCCTATGTGGTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCA15360.12787819601829256No Hit
GTGTCAGTGGCCCCAGGACAGACGGCCAAGATTACTTGTGTCGGAAACGA15340.12771168795056043No Hit
CTCCTGTGCAGGCTCTGGATTCACGTTCAGTGCCTATGTCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG15330.12762843391669437No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT15280.12721216374736397No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14940.12438152659591739No Hit
CTCCTATGTGGTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAAGATTACTTGTGTCGGAA14930.12429827256205132No Hit
CATCATGGACGACCAGTACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAG14910.12413176449431916No Hit
ATCCCAGACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGGCCGTGTTCCC14880.12388200239272093No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC14740.12271644591859587No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14680.12221692171539941No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC14440.1202188249026136No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACA14390.11980255473328322No Hit
GTCCTGGGGCCACTGACACCGAGGGTGGCTGAGTCACCACATAGGAGGTCACAGAGGCTGTGCAGTGAGAGAGG14330.11930303053008676No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG14320.11921977649622068No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG14020.11672215548023841No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCC14010.11663890144637233No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGCCTCTGTGACCTC13910.11580636110771157No Hit
CCTGAGGGCCGGTCGCCATCATCATGGACGACCAGTACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGTAC13730.11430778849812222No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC13680.11389151832879181No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGGG13460.11205992958373816No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT13450.11197667554987208No Hit
GACTATTACTGTCAGGTCTGGGATAGTAGTAGTGATCATTATGTCTTCGGAGCTGGGACCAAGGCCACCGTCCT13100.10906278436455942No Hit
GGATAGTAGTAGTGATCATTATGTCTTCGGAGCTGGGACCAAGGCCACCGTCCTAGGTCAGCCCAAGGCCAACCC13040.10856326016136296No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG12980.10806373595816651No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCC12900.10739770368723792No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT12870.10714794158563969No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC12720.10589913107764855No Hit
GTGTACACTTTTAATTCCAATGTCGTTTCCGACACAAGTAATCTTGGCCGTCTGTCCTGGGGCCACTGACACCG12710.10581587704378247No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCC12640.10523309880671994No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAG12360.10290198585846981No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12040.10023785677475539No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)