FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1201143
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTGTACTGGTCGTCCATGATGATGGCGACCGGCCCTCAGGGATCCCT75780.6308990686371231No Hit
GTACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGTACACTTTTAATT66050.5498928936854313No Hit
CCTATGTGGTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAAGATTACTTGTGTCGGAAACG47560.395956185067057No Hit
ACCTAGGACGGTGGCCTTGGTCCCAGCTCCGAAGACATAATGATCACTACTACTATCCCAGACCTGACAGTAAT44460.37014743456857346No Hit
GACCTAGGACGGTGGCCTTGGTCCCAGCTCCGAAGACATAATGATCACTACTACTATCCCAGACCTGACAGTAAT44420.36981441843310914No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC43070.3585751238611889No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT41780.3478353534924651No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGGCCTTGGTCCC40970.34109177674931296No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38510.3206112844182583No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC38410.31977874407959755No Hit
GTAATATACGGCCGTGTCGTCGGCTCTCAGGCTGGTCATTTGCAGAGACAGCGTCTTCTTGGAAAGGTCTCTGG36900.30720738496582006No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG35960.2993815057824089No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC35790.29796618720668566No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC31160.2594195695266925No Hit
CCCCAGGACAGACGGCCAAGATTACTTGTGTCGGAAACGACATTGGAATTAAAAGTGTACACTGGTACCAGCAGA31120.2590865533912282No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28610.23818979089084316No Hit
CCTCTCTCACTGCACAGCCTCTGTGACCTCCTATGTGGTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAC28470.23702423441671808No Hit
GCCTCTGTGACCTCCTATGTGGTGACTCAGCCACCCTCGGTGTCAGTGGC28440.23677447231511983No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGCCTCTGTGACCTCCTATGTGGTGACT27850.2318624843170214No Hit
CTACTACTATCCCAGACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTC27010.22486914547227102No Hit
GTATATTACTGTGCGAAAGGAGGCGGATTGATAGGGGATGAAACTAGTGG26960.2244528753029406No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTC26760.2227877946256191No Hit
GCCTTGGGCTGACCTAGGACGGTGGCCTTGGTCCCAGCTCCGAAGACATAATGATCACTACTACTATCCCAGAC26320.21912461713551176No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGT26300.2189581090677796No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGCCTCTGTG24990.20805183063132365No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCCATCATCATGG24740.20597047978467178No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG23700.19731206026259987No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGGCCTTGGTCCCAGCTCCGAAGACATAATGATC23410.19489769328048367No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTC23410.19489769328048367No Hit
TAGTAGTAGTGATCATTATGTCTTCGGAGCTGGGACCAAGGCCACCGTCCTAGGTCAGCCCAAGGCCAACCCC23250.19356562873862646No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGCCTCTGTGACCTCCTATGTGGTGACTCAGC22380.18632252779227787No Hit
GTACTGGTCGTCCATGATGATGGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAAC22150.1844076850133581No Hit
CTCACTGCACAGCCTCTGTGACCTCCTATGTGGTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG21760.18116077769258115No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGCCTCTGTGACCTCCTATGTGGTGACTCAGCC21030.17508323322035763No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG20380.16967172101906267No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGCCTCTGTGACCTCCTA20330.16925545084973231No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGACCATCAGCAGGGTCGA20260.16867267261266974No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT20110.16742386210467863No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19860.1653425112580267No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT19850.16525925722416065No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGG19660.16367743058070522No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGGTCTGGGATAGTAGT19650.16359417654683914No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG19420.1616793337679194No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTG19400.16151282570018724No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGA19370.16126306359858902No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGG19250.1602640151921961No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG18780.15635107560049052No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC18700.15568504332956193No Hit
CTACTATCCCAGACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGAC18540.1543529787877047No Hit
GATTACTTGTGTCGGAAACGACATTGGAATTAAAAGTGTACACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGT18470.1537702005506422No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGG18370.1529376602119814No Hit
GTAGTAGTGATCATTATGTCTTCGGAGCTGGGACCAAGGCCACCGTCCTAGGTCAGCCCAAGGCCAACCCC18340.1526878981103832No Hit
CTGCACAGCCTCTGTGACCTCCTATGTGGTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCA18080.1505232932298652No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGCCTCTGTGACCTC17020.14169836564006116No Hit
GACCAGTACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGTACACTTTTAATTCCAATGTCGTTTCCGACACA16700.13903423655634675No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTG16350.1361203453710341No Hit
GTGTACACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTACTGGTCGTC15930.1326236759486589No Hit
CTCCTGTGCAGGCTCTGGATTCACGTTCAGTGCCTATGTCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG15920.13254042191479282No Hit
GTGTCAGTGGCCCCAGGACAGACGGCCAAGATTACTTGTGTCGGAAACGA15540.12937676862788194No Hit
CACTACTACTATCCCAGACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG15490.12896049845855156No Hit
ATCCCAGACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGGCCGTGTTCCC15420.12837772022148902No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCCATTTTAAAAGGTGTCC15400.1282112121537569No Hit
TCCATGATGATGGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACC15320.12754517988282826No Hit
CCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAAGATTACTTGTGTCGGAAACGACATTGGAATTAAAAGTGT15270.12712890971349788No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC15270.12712890971349788No Hit
CTCCTATGTGGTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAAGATTACTTGTGTCGGAA15260.1270456556796318No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCCAG14770.1229662080201941No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT14670.12213366768153333No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14560.12121787330900649No Hit
CTATTACTGTCAGGTCTGGGATAGTAGTAGTGATCATTATGTCTTCGGAG14230.118470490191426No Hit
GGCCAGGCCCCTGTACTGGTCGTCCATGATGATGGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCC13870.11547334497224726No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACA13710.11414128043039004No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13640.11355850219332753No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC13550.11280921588853283No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC13480.1122264376514703No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT13120.10922929243229157No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC13070.10881302226296119No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG12920.10756421175497005No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCC12610.10498333670512171No Hit
GTCCTGGGGCCACTGACACCGAGGGTGGCTGAGTCACCACATAGGAGGTCACAGAGGCTGTGCAGTGAGAGAGG12560.10456706653579134No Hit
GTACTGGTCGTCCATGATGATGGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGA12460.10373452619713056No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG12410.10331825602780019No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC12400.10323500199393412No Hit
CATCATGGACGACCAGTACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAG12300.10240246165527335No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT12150.10115365114728221No Hit
GACTATTACTGTCAGGTCTGGGATAGTAGTAGTGATCATTATGTCTTCGGAGCTGGGACCAAGGCCACCGTCCT12140.10107039711341613No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCC12050.10032111080862147No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12040.10023785677475539No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)