FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1205835
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAAACAGCCGTGTCCTCGGCTCTCAGGCTGTCCATCTGAAGATAGAGGGTGTTCTTGGAACTGTCTCTGG46700.38728350064478145No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT37250.3089145695721222No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36220.3003727707356313No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG35330.29299199310021684No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG30490.2528538315772888No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGC28840.23917036742174508No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCG26860.2227502104350927No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26000.21561822305705175No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG25130.20840330559321962No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG24380.20218354915888162No Hit
GTTTACTACTGTGCGAGTGACTATAATCTGGGCTACTGGGGCCAGGGAAC23490.19480277152346714No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG22980.19057333714811728No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22590.1873390638022615No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT22010.1825291188263734No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21340.1769728030783648No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC20480.16984081570032386No Hit
CCCTAAGACTCTCCTGTGCAGCCTCTGGATTCACCGTTGGTCAAAATTAC20480.16984081570032386No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA20110.1667724025260504No Hit
AGTCAGACCATTGCCAATTATTTAAATTGGTATCAGCAAAAACCAGGGAA19710.16345519909440345No Hit
GATCTATGTTGCATCTAATTTACAAAGTGGGGTCCCATCAAGGTTTAGTGGCAGTGGATCTGGGACAGATTTC19370.16063557617750354No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA18430.15284014811313323No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18410.15267428794155088No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG17910.14852778365199218No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17880.14827899339461867No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAATGTGCGGGGGTTAATGAAAGTCTGTTGAC17820.14778141287987162No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC17120.14197630687448948No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16950.1405664954160395No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCTTGTATTTCCAGGAGAAAGTGATGG16940.14048356533024833No Hit
ACATAGATCAGGAGGTTGGGGGCTTTCCCTGGTTTTTGCTGATACCAATT16830.13957133438654543No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCC16480.13666878138385433No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCTGAACCTGCGGGAGTCGGCCACCATC16360.13567362035436026No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT16250.13476138941065735No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC16150.1339320885527456No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC15510.12862456306211048No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCGTCCCTGTCTGCTTCTGT15020.12456098885834296No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAG14800.12273652697093716No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC14520.12041448456878427No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT14450.11983397396824608No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTATTACTGTCAACAGACTTTCATTAAC14320.11875588285296082No Hit
GAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGT14230.11800951208084025No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTGCCAATTATTTAAATTGGTATCAGCAAAA14140.11726314130871968No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14130.11718021122292852No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13990.11601919002185207No Hit
ACTTATTACTGTCAACAGACTTTCATTAACCCCCGCACATTCGGCCAAGGGACCAAGGTGGAAATCAAACGA13670.11336542727653452No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC13650.11319956710495217No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13560.1124531963328316No Hit
CTCCTGTGCAGCCTCTGGATTCACCGTTGGTCAAAATTACATGGAGTGGGTTCGCCAGGCTCCAGGGAAGGGG13530.11220440607545808No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT13460.11162389547491987No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13110.10872134247222878No Hit
ATGTTGCATCTAATTTACAAAGTGGGGTCCCATCAAGGTTTAGTGGCAGTGGATCTGGGACAGATTTCACTCTC13100.10863841238643761No Hit
GATCTGCACTGAACACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGG13040.10814083187169057No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12840.10648223015586711No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC12790.10606757972691123No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTATTACTGTCAACAGACTTTCATTAACCCCCGCACATT12740.10565292929795536No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCACGGCG12510.10374553732475837No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT12510.10374553732475837No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12180.10100884449364962No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCCAGATTATAGTCACTCGCACAGTAGTAAACAGC12160.10084298432206729No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)