FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1298136
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT70690.5445500317378149No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT63920.49239833114558107No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC54490.41975571126599986No Hit
CCTCATATATGATGCGTCCCTCAGGGCCGCTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGAGACAGACTT49760.3833188510294761No Hit
ATGTACGCTTTTGGCCAGGGGACCAAGCTGGAGCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG46570.35874515459089035No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44440.3423370124547813No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAACGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAAGA44390.34195184479900415No Hit
GTCTGAGACAGACTTCACTCTCACCATCAGCAGCCTGGAGCCTGAAGATT41200.31737814836041833No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40750.3139116394584235No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG39850.30697862165443374No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39240.3022795762539518No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG38170.2940369884203196No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA37310.28741210474095163No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT35530.27370013619528305No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAAGAACTATTTAGGCTGGTACCAACAAAAATCTGGCCAGGCTCCCAGGCT35310.2720053985098634No Hit
CCTCAGGGCCGCTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGAGACAGACTTCACTCTCACCATCAGC34800.26807668842093585No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA32570.2508982109732725No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG32150.24766280266474394No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC30630.23595370592911685No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA30130.23210202937134478No Hit
GTCTCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGCGGCCCTGAG29520.22740298397086284No Hit
GTCTTTGTCTCCAGGGGAACGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAAGAACTATTTAGGCTGGT28990.22332020681962444No Hit
GTGCAGCCACAGTTCGTTTGAGCTCCAGCTTGGTCCCCTGGCCAAAAGCGTACATAGACCAGTTGCTACGCTG27100.20876086943124603No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC26930.20745129940160353No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26450.20375368990614234No Hit
AGCGTACATAGACCAGTTGCTACGCTGCTGACAGTAATAAACTCCAAAAT26230.20205895222072265No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25940.19982497981721484No Hit
TATATGAGGAGCCTGGGAGCCTGGCCAGATTTTTGTTGGTACCAGCCTAAATAGTTCTTAACACTCTGACTGGCC25720.19813024213179514No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGCTCCAGCTTGGTCCCCTGGCCAAAAGCGTACATAGACC25250.19450966616748938No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25120.19350823026246863No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC24780.19088909020318365No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC24710.19034985548509556No Hit
CATATATGATGCGTCCCTCAGGGCCGCTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGAGACAGACTTC24430.1881929166127432No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG23780.1831857370876395No Hit
GCGTAGCAACTGGTCTATGTACGCTTTTGGCCAGGGGACCAAGCTGGAGCTCAAACGAACTGTGGCTGCACCAT22640.1744039145359192No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT22540.1736335792243648No Hit
GCCCTGAGGGACGCATCATATATGAGGAGCCTGGGAGCCTGGCCAGATTTTTGTTGGTACCAGCCTAAATAGTT22510.17340247863089844No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC22440.17286324391281035No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT22050.16985893619774817No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC22000.16947376854197094No Hit
TATATGATGCGTCCCTCAGGGCCGCTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGAGACAGACTTCACT22000.16947376854197094No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21490.16554505845304343No Hit
GACTTCACTCTCACCATCAGCAGCCTGGAGCCTGAAGATTTTGGAGTTTA21380.1646976896103336No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21210.16338811958069108No Hit
TCATATATGAGGAGCCTGGGAGCCTGGCCAGATTTTTGTTGGTACCAGCC20970.1615393148329605No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTTTCTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCC20420.15730247061941122No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20410.15722543708825576No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC19860.1529885928747065No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19600.15098572106466504No Hit
ATTTAGGCTGGTACCAACAAAAATCTGGCCAGGCTCCCAGGCTCCTCATA19410.14952208397271163No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT19270.14844361453653548No Hit
CTATTTAGGCTGGTACCAACAAAAATCTGGCCAGGCTCCCAGGCTCCTCATATATGATGCGTCCCTCAGGGCCG19060.1468259103822712No Hit
GTTCAGTGGCAGTGGGTCTGAGACAGACTTCACTCTCACCATCAGCAGCC18910.14567040741493956No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18850.14520820622800693No Hit
GAGTGAAGTCTGTCTCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA18530.1427431332310328No Hit
GGCCAAAAGCGTACATAGACCAGTTGCTACGCTGCTGACAGTAATAAACTCCAAAATCTTCAGGCTCCAGGCTG18440.14204983145063382No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT17990.13858332254863898No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC17980.13850628901748355No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17800.1371196854566856No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC17490.1347316459908669No Hit
ACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAAGAACTATTTAGGCTGGTACCAACAAAAATCTGGCCAGGCTCCC17200.13249767358735912No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17130.131958438869271No Hit
CCCAGATACCACCGGAGAAATTTTCTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAACGAGCCAC16720.12880006409189793No Hit
CACCATCAGCAGCCTGGAGCCTGAAGATTTTGGAGTTTATTACTGTCAGCAGCGTAGCAACTGGTCTATGTACG16720.12880006409189793No Hit
CTGCAGGAGAGGGTGGCTCGTTCCCCTGGAGACAAAGACAGGGTGGCTGG16210.12487135400297042No Hit
GGTCTATGTACGCTTTTGGCCAGGGGACCAAGCTGGAGCTCAAACGAACT16100.12402398516026057No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT16050.12363881750448336No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15880.12232924747484085No Hit
GCCTGGGAGCCTGGCCAGATTTTTGTTGGTACCAGCCTAAATAGTTCTTAACACTCTGACTGGCCCTGCAGGAG15870.12225221394368542No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15780.12155891216328643No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15580.12001824154017761No Hit
GTTAAGAACTATTTAGGCTGGTACCAACAAAAATCTGGCCAGGCTCCCAGGCTCCTCATATATGATGCGTCCCTC15510.1194790068220895No Hit
ATATATGAGGAGCCTGGGAGCCTGGCCAGATTTTTGTTGGTACCAGCCTAAATAGTTCTTAACACTCTGACTGGC15430.11886273857284599No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC15290.11778426913666981No Hit
GTTTATTACTGTCAGCAGCGTAGCAACTGGTCTATGTACGCTTTTGGCCAGGGGACCAAGCTGGAGCTCAAACGA15230.11732206794973715No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC14910.11485699495276305No Hit
GAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTC14740.11354742492312053No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14690.11316225726734333No Hit
GTAATAAACTCCAAAATCTTCAGGCTCCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCTCAGACCCACTGCC14670.11300819020503244No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14580.11231488842463348No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14540.11200675430001171No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG14420.1110823519261464No Hit
CATCATATATGAGGAGCCTGGGAGCCTGGCCAGATTTTTGTTGGTACCAGCCTAAATAGTTCTTAACACTCTG14240.10969574836534847No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA14190.10931058070957125No Hit
CCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTTTCTTGACACAGTCTC14180.10923354717841582No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT14100.10861727892917229No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13940.10738474243068521No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13710.10561297121411006No Hit
CTCCCAGGCTCCTCATATATGATGCGTCCCTCAGGGCCGCTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTG13660.10522780355833286No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13570.1045345017779339No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC13560.10445746824677844No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13450.1036100994040686No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13280.10230052937442609No Hit
TTATTACTGTCAGCAGCGTAGCAACTGGTCTATGTACGCTTTTGGCCAGGGGACCAAGCTGGAGCTCAAACGA13200.10168426112518256No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13160.10137612700056081No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTC13060.1006057916890064No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)