FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004717780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1298136
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT67950.523442844201224No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT55810.42992413737851815No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC49670.38262554924907716No Hit
CCTCATATATGATGCGTCCCTCAGGGCCGCTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGAGACAGACTT45490.35042553322610265No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44100.33971787239549633No Hit
ATGTACGCTTTTGGCCAGGGGACCAAGCTGGAGCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG42230.32531260206942875No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA39170.3017403415358637No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT38950.30004560385044404No Hit
GTCTGAGACAGACTTCACTCTCACCATCAGCAGCCTGGAGCCTGAAGATT38660.29781163144693623No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAACGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAAGA36680.28255899227815884No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36520.2813264557796718No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG36490.28109535518620543No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAAGAACTATTTAGGCTGGTACCAACAAAAATCTGGCCAGGCTCCCAGGCT33160.25544318931144344No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT32000.2465072996974123No Hit
CCTCAGGGCCGCTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGAGACAGACTTCACTCTCACCATCAGC31400.24188528782808583No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA30210.23271829762058827No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG28730.22131733500958295No Hit
GTCTTTGTCTCCAGGGGAACGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAAGAACTATTTAGGCTGGT28070.21623312195332384No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC27590.21253551245786267No Hit
AGCGTACATAGACCAGTTGCTACGCTGCTGACAGTAATAAACTCCAAAAT27350.21068670771013207No Hit
GTCTCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGCGGCCCTGAG27070.20852976883777968No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26880.20706613174582633No Hit
GTGCAGCCACAGTTCGTTTGAGCTCCAGCTTGGTCCCCTGGCCAAAAGCGTACATAGACCAGTTGCTACGCTG26850.20683503115236No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26730.20591062877849473No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG26540.20444699168654132No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC25660.19766804094486248No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC24340.1874996148323442No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC24300.18719148070772246No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC24210.18649817892732348No Hit
TATATGAGGAGCCTGGGAGCCTGGCCAGATTTTTGTTGGTACCAGCCTAAATAGTTCTTAACACTCTGACTGGCC23790.18326277061879495No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA23630.1820302341203079No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC23160.17840965815600215No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGCTCCAGCTTGGTCCCCTGGCCAAAAGCGTACATAGACC23090.17787042343791407No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT23030.1774082222509814No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT22410.17263214331934404No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22030.16970486913543725No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT21670.16693166201384138No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21580.16623836023344243No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTTTCTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCC21360.1645436225480227No Hit
CATATATGATGCGTCCCTCAGGGCCGCTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGAGACAGACTTC21140.162848884862603No Hit
GCCCTGAGGGACGCATCATATATGAGGAGCCTGGGAGCCTGGCCAGATTTTTGTTGGTACCAGCCTAAATAGTT20740.15976754361638534No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG20720.15961347655407446No Hit
GCGTAGCAACTGGTCTATGTACGCTTTTGGCCAGGGGACCAAGCTGGAGCTCAAACGAACTGTGGCTGCACCAT20690.1593823759606081No Hit
GACTTCACTCTCACCATCAGCAGCCTGGAGCCTGAAGATTTTGGAGTTTA20520.15807280593096565No Hit
TATATGATGCGTCCCTCAGGGCCGCTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGAGACAGACTTCACT19890.15321969346817282No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19180.14775031275613648No Hit
TCATATATGAGGAGCCTGGGAGCCTGGCCAGATTTTTGTTGGTACCAGCC19030.14659480978880488No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT18740.1443608373852971No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18730.14428380385414163No Hit
GGCCAAAAGCGTACATAGACCAGTTGCTACGCTGCTGACAGTAATAAACTCCAAAATCTTCAGGCTCCAGGCTG18450.14212686498178928No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC18410.1418187308571675No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18180.14004695964059236No Hit
GAGTGAAGTCTGTCTCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA18130.13966179198481515No Hit
ATTTAGGCTGGTACCAACAAAAATCTGGCCAGGCTCCCAGGCTCCTCATA18110.13950772492250427No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC17960.13835222195517266No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC17850.1375048531124628No Hit
GTTCAGTGGCAGTGGGTCTGAGACAGACTTCACTCTCACCATCAGCAGCC17570.13534791424011045No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17300.13326800889891352No Hit
GGTCTATGTACGCTTTTGGCCAGGGGACCAAGCTGGAGCTCAAACGAACT17040.13126513708887205No Hit
CTATTTAGGCTGGTACCAACAAAAATCTGGCCAGGCTCCCAGGCTCCTCATATATGATGCGTCCCTCAGGGCCG16940.13049480177731765No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16800.12941633234114144No Hit
CACCATCAGCAGCCTGGAGCCTGAAGATTTTGGAGTTTATTACTGTCAGCAGCGTAGCAACTGGTCTATGTACG16080.12386991809794969No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC15930.12271441513061807No Hit
GCCTGGGAGCCTGGCCAGATTTTTGTTGGTACCAGCCTAAATAGTTCTTAACACTCTGACTGGCCCTGCAGGAG15720.1210967109763538No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15620.12032637566479938No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15590.12009527507133304No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG15490.11932493975977865No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15420.11878570504169056No Hit
ACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAAGAACTATTTAGGCTGGTACCAACAAAAATCTGGCCAGGCTCCC15250.11747613501204804No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15200.11709096735627084No Hit
ATATATGAGGAGCCTGGGAGCCTGGCCAGATTTTTGTTGGTACCAGCCTAAATAGTTCTTAACACTCTGACTGGC15190.11701393382511541No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15140.11662876616933819No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC14960.11524216260854026No Hit
CTGCAGGAGAGGGTGGCTCGTTCCCCTGGAGACAAAGACAGGGTGGCTGG14890.11470292789045215No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14710.11331632432965422No Hit
CCCAGATACCACCGGAGAAATTTTCTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAACGAGCCAC14590.11239192195578891No Hit
GAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTC14230.10961871483419303No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14150.10900244658494951No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA14130.10884837952263862No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14030.1080780442110842No Hit
GTTAAGAACTATTTAGGCTGGTACCAACAAAAATCTGGCCAGGCTCCCAGGCTCCTCATATATGATGCGTCCCTC14030.1080780442110842No Hit
GTAATAAACTCCAAAATCTTCAGGCTCCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCTCAGACCCACTGCC13920.10723067536837436No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC13870.10684550771259714No Hit
CTCCCAGGCTCCTCATATATGATGCGTCCCTCAGGGCCGCTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTG13820.10646034005681994No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13690.1054589041517992No Hit
GTTTATTACTGTCAGCAGCGTAGCAACTGGTCTATGTACGCTTTTGGCCAGGGGACCAAGCTGGAGCTCAAACGA13660.10522780355833286No Hit
GTTGCTACGCTGCTGACAGTAATAAACTCCAAAATCTTCAGGCTCCAGGCTGCTGATGGTGAGAGTGAAGT13520.1041493341221567No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCC13350.10283976409251419No Hit
CTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTTTCTT13350.10283976409251419No Hit
CCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTTTCTTGACACAGTCTC13080.10075985875131728No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)