FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1312749
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGCTGGCTCACTCACTTTCGGCGGAGGGACCAAGGTGGAGCTCAAACGAACTGTGG55060.41942519095424946No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47940.3651878615028463No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40530.30874142734064164No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT38990.2970103195660404No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC38070.2900021253110838No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC33480.25503733006081136No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29990.22845189750668257No Hit
GTGTATTACTGTCAGCAGTATGCTGGCTCACTCACTTTCGGCGGAGGGAC28300.21557814936442535No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA27330.20818907498691674No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT26420.20125705675647057No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26160.1992764801192002No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25560.1947059186485764No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGGAGCAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCA24900.18967830103089015No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG24520.18678361209949504No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTT24240.1846506834132039No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT23150.17634749674157055No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC22480.1712437030993739No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGG22070.1681204860944476No Hit
GTGTTAGGAGCAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAAGATCCTCATCTATGGCGCAT21760.16575902933462527No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG21470.16354992462382373No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGGAGCA20760.1581414268835855No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG20350.15501820987865922No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20030.15258057709432649No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC18810.14328710210405798No Hit
GTGCAGCCACAGTTCGTTTGAGCTCCACCTTGGTCCCTCCGCCGAAAGTGAGTGAGCCAGCATACTGCTGACAGT18270.13917359678049648No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18100.13787860436381974No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA17660.13452685928536226No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC17390.1324701066235815No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA17320.13193687445200872No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC17160.13071805805984235No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC17160.13071805805984235No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC17060.1299562978147384No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16830.12820424925099924No Hit
ATGCTGGCTCACTCACTTTCGGCGGAGGGACCAAGGTGGAGCTCAAACGA16720.12736631298138487No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16370.12470015212352095No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16310.12424309597645856No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGGCCGCTCAGTTCAATGTCGGCTGTGCTCGTGGATTCGTCCGCGCT16170.12317663163331298No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGCTGGCTC16040.12218634331467783No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC15770.12012959065289708No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTTGGATGCGCCAT15770.12012959065289708No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15230.11601608532933562No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGCTCCACCTTGGTCCCTCCGCCGAAAGTGAGTGAGCCAGC15220.11593990930482523No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAATTTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCC14670.11175022795675335No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT14490.11037905951556619No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGTGAGCCAGCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCC14470.1102267074665454No Hit
GACCAAGGTGGAGCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA14240.10847465890280626No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT14000.10664643431455671No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13890.10580849804494234No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC13710.10443732960375518No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13160.1002476482556833No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)