FastQCFastQC Report
Wed 8 May 2024
EGAF00004717784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1498129
Total Bases110.5 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC72990.4872077104174607No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGATCTGTTA70930.4734572256461226No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC70790.4725227266810802No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC68710.45863874205759314No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT68700.45857199213151867No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG61740.4121140435836967No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC61510.4105787952839842No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC60070.4009668059292625No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT56040.37406658572125634No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCT52930.35330735871210023No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC51230.34195987127944255No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA49440.3300116345121148No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCC46120.3078506590553951No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC45720.30518066201241684No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGAGTGATTATTACTGCAGCTCATAT45470.30351191386055537No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGT44860.2994401683700135No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT41650.27801344210011286No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA41390.276277944022177No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATA40280.2688687022279123No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA39540.26392920769840245No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA38540.2572542150909568No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT38140.25458421804797854No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG37630.2511799718181812No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT37160.24804272529268173No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA35380.2361612384514284No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG35190.23489298985601373No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT34270.2287519966571637No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCT33530.22381250212765388No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT33500.22361225234943052No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGATCTGTTAC33070.22074200552822887No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA32990.22020800611963323No Hit
GGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAA32550.2172710093723571No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA32010.21366651336433645No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT31980.2134662635861131No Hit
GTAATAATCACTCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG31820.21239826476892176No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT31270.20872701883482664No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT30600.20425477378783805No Hit
GATTATTACTGCAGCTCATATACAAGTAACAGATCTGTGGTATTCGGCGG30320.20238577585775325No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC29790.19884802977580704No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29650.1979135308107646No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACATGGAGCCAGAGAAG29120.19437578472881842No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC28630.19110503835117004No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC28440.18983678975575535No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGG28280.18876879093856405No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT28130.18776754204744717No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA27170.1813595491442993No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT27080.1807587998096292No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG26370.17601955505834277No Hit
GTATATGAGCTGCAGTAATAATCACTCTCGTCCTCAGCCTGGAGCCCAGA25940.17314930823714114No Hit
GTAATAGACGGCCGTGTCGTCAGATCTCAGGCTGCTCAGCTCCATGTACG25880.1727488086806944No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC25590.17081306082453515No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA24760.1652728169603552No Hit
GTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGC24650.16453856777353618No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT24610.16427156806923837No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT24090.1608005719133666No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC24000.1601998225786965No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG23740.15846432450076062No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG23540.15712932597927148No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC23280.1553938279013356No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG23090.15412557930592094No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC22770.15198958167153828No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA22520.15032083351967687No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTC22310.1489190850721133No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACATGGAGCCAGAGAAGCGATT22270.14865208536781546No Hit
GTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCG22080.14738383677240077No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACATGGAGCC21490.14344559113400782No Hit
CCCCTGAGGGCCGATTATTGACATCATAAATCATGAGTTTGGGGGCTTTG21460.14324534135578443No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC21330.14237759231681651No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA21290.14211059261251868No Hit
GAATACCACAGATCTGTTACTTGTATATGAGCTGCAGTAATAATCACTCT21160.14124284357355074No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC20760.13857284653057247No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG20740.13843934667842353No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC20470.1366370986744132No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA20280.13536885007899854No Hit
GCTTGGTCCCTCCGCCGAATACCACAGATCTGTTACTTGTATATGAGCTG20280.13536885007899854No Hit
GACCATCTCTGGGCTCCAGGCTGAGGACGAGAGTGATTATTACTGCAGCT19820.13229835347957353No Hit
CTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCC19650.13116360473630775No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTC19500.1301623558451909No Hit
CCACAGATCTGTTACTTGTATATGAGCTGCAGTAATAATCACTCTCGTCC19480.13002885599304198No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT19140.12775935850651043No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA19030.1270251093196914No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACA18930.12635761005894686No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCC18840.12575686072427675No Hit
GATCTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC18790.12542311109390447No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG18750.12515611138960664No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGG18400.12281986397700065No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC18260.12188536501195825No Hit
CAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCAATAA18230.12168511523373488No Hit
GGTCAGGGAGGCCGTGTTGCCAGACATGGAGCCAGAGAAGCGATTAGAAA18120.12095086604691585No Hit
GTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGACATAGTTATAA17890.11941561774720334No Hit
CTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCG17850.11914861804290552No Hit
TCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGC17790.11874811848645878No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT17450.11647862099992724No Hit
GTGTTGTTGGTACCAGGAGACATAGTTATAACCACCAACGTCACTGCTGG17440.1164118710738528No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGG17300.1154773721088104No Hit
GCTCATATACAAGTAACAGATCTGTGGTATTCGGCGGAGGGACCAAGCTG17240.11507687255236365No Hit
GTCAATAATCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCATGTC17160.11454287314376799No Hit
CTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCC17140.11440937329161907No Hit
CTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGA17000.11347487432657667No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGC16820.11227337565723645No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16770.11193962602686419No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGT16750.11180612617471526No Hit
GGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTC16590.11073812735752395No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGT16560.11053787757930057No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAA16500.11013737802285384No Hit
GTCTATTACTGTGCGACAAAATTGTCGTACCAGTTGGTATGGGGGGGCTA16450.10980362839248155No Hit
CTCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCC16380.10933637890996036No Hit
GGACGAGAGTGATTATTACTGCAGCTCATATACAAGTAACAGATCTGTGG16360.10920287905781144No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA16340.10906937920566254No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA16320.10893587935351362No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA16290.10873562957529025No Hit
GAGCCAGAGAAGCGATTAGAAACCCCTGAGGGCCGATTATTGACATCATA16120.10760088083202447No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT16000.10679988171913099No Hit
GTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAA15970.10659963194090763No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC15930.10633263223660981No Hit
GGCAAAGCCCCCAAACTCATGATTTATGATGTCAATAATCGGCCCTCAGG15890.10606563253231197No Hit
CTCTGGCTCCATGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCC15780.10533138334549294No Hit
GCTCCAGGCTGAGGACGAGAGTGATTATTACTGCAGCTCATATACAAGTA15710.10486413386297175No Hit
GGCTGAGGACGAGAGTGATTATTACTGCAGCTCATATACAAGTAACAGAT15700.1047973839368973No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC15560.1038628849718549No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT15520.10359588526755706No Hit
GGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGAC15420.1029283860068125No Hit
GTATCAACGCAGAGTACGGGGGGGTCTCAGGAGGCAGCACTCTCGGGACG15350.10246113652429131No Hit
ATTATTGACATCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTT15310.10219413681999347No Hit
GTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCA15150.10112613800280217No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG15150.10112613800280217No Hit
GATCTGTTACTTGTATATGAGCTGCAGTAATAATCACTCTCGTCCTCAGC15130.10099263815065325No Hit
AGGCAGCACTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCT15070.10059213859420651No Hit
GGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGG15060.10052538866813206No Hit
GCCAGACATGGAGCCAGAGAAGCGATTAGAAACCCCTGAGGGCCGATTAT15050.10045863874205758No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)